CN-122012338-A - Pseudomonas antagonizing eucalyptus bacterial wilt and application thereof

Abstract

The invention discloses pseudomonas for antagonizing eucalyptus bacterial wilt and application thereof, and belongs to the technical field of microorganisms. The Pseudomonas sp. SHP-38 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2026, 1 month and 19 days and a preservation number of CGMCC No.37456. The invention confirms that the pseudomonas SHP-38 belongs to a new species of pseudomonas through physiological biochemical identification and molecular taxonomy identification, and expands species resources and genetic diversity of pseudomonas flora. The pseudomonas SHP-38 has the double effects of inhibiting the growth of the bacterial wilt of eucalyptus and promoting the growth of plants, has the potential of being developed into a microbial agent for the bacterial wilt of eucalyptus, is beneficial to reducing the use of chemical pesticides, provides a novel biological control technology for solving the bacterial wilt of eucalyptus, and has wide application prospect.

Inventors

• FAN XINLEI
• LI RUIFANG
• JIANG YUHAN
• ZHANG WEISHAN

Assignees

• 北京林业大学

Dates

Publication Date

20260512

Application Date

20260227

Claims (10)

1. 1. The Pseudomonas sp.) SHP-38 is characterized by being preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2026, 1 month and 19 days, a preservation number of CGMCC No.37456, and a preservation address of North Star, xila No. 1, 3 national academy of sciences of China, which is the Korean region of Beijing.
2. 2. The use of pseudomonas SHP-38 according to claim 1 for the preparation of a microbial agent for antagonizing ralstonia eucalypti.
3. 3. A microbial agent that antagonizes ralstonia eucalypti, comprising pseudomonas SHP-38 of claim 1, a fermentation broth of pseudomonas SHP-38, or a metabolite of pseudomonas SHP-38.
4. 4. Use of the pseudomonas SHP-38 of claim 1 or the microbial agent of claim 3 for inhibiting the growth of ralstonia eucalypti.
5. 5. Use of the pseudomonas SHP-38 of claim 1 or the microbial agent of claim 3 for controlling bacterial wilt of plants.
6. 6. Use of the pseudomonas SHP-38 of claim 1 or the microbial agent of claim 3 for the preparation of a product for controlling bacterial wilt of plants.
7. 7. Use of pseudomonas SHP-38 according to claim 1 or a microbial agent according to claim 3 for promoting plant growth.
8. 8. Use of pseudomonas SHP-38 according to claim 1 or a microbial agent according to claim 3 for the preparation of a plant growth promoter.
9. 9. The use according to any one of claims 5 to 8, wherein the plant comprises eucalyptus.
10. 10. A product for controlling bacterial wilt of plants, characterized in that the product comprises the pseudomonas SHP-38 according to claim 1 or the microbial agent according to claim 3.

Description

Pseudomonas antagonizing eucalyptus bacterial wilt and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to pseudomonas for antagonizing eucalyptus bacterial wilt and application thereof. Background Eucalyptus (Eucalyptus spp.) is a generic term for plants of the genus Eucalyptus (Eucalyptus) of the family Myrtaceae. As one of three world woods, eucalyptus has strong environmental adaptability and ecological stability, so that the eucalyptus is widely introduced into a plurality of regions worldwide for cultivation. Bacterial wilt of eucalyptus is a systemic vascular bundle bacterial disease caused by infection with the strain Lawsonia solanaceae (Ralstonia solanacearum). The pathogenic bacteria can invade the root system of the host through soil, and then systematically spread in the vascular bundle tissue of the plant, so that the host has acute wilting symptoms and finally die integrally. The disease severely restricts the eucalyptus industry development and has caused significant economic losses. The traditional chemical control method not only pollutes the environment, but also can cause pathogenic bacteria to generate drug resistance after long-term use. Therefore, the development of a more green, safer and more efficient biocontrol method by utilizing the microorganism strain with biocontrol capability becomes an important research point. Disclosure of Invention The invention aims to provide pseudomonas for antagonizing eucalyptus bacterial wilt and application thereof, so as to solve the problems in the prior art. The invention discloses a new kind of microorganism Pseudomonas (Pseudomonas sp.) SHP-38 with antagonistic action on the bacterial wilt of eucalyptus, which expands species resources and genetic diversity of Pseudomonas flora. The pseudomonas SHP-38 has the double effects of inhibiting the growth of the bacterial wilt of eucalyptus and promoting the growth of plants, has the potential of being developed into a microbial agent for the bacterial wilt of eucalyptus, is favorable for reducing the use of chemical pesticides, is favorable for environmental protection and sustainable development, provides a novel biological control technology for solving the bacterial wilt of eucalyptus, and has wide application prospect. In order to achieve the above object, the present invention provides the following solutions: The invention provides a Pseudomonas sp SHP-38 which is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2026, 1 month and 19 days, a preservation number of CGMCC No.37456 and a preservation address of North Star Xiyang area of Beijing, national academy of sciences of China, microbiological culture Collection of China, and a China center No. 3. The invention also provides application of the pseudomonas SHP-38 in preparing a microbial agent for antagonizing the bacterial wilt of eucalyptus. The invention also provides a microbial agent for antagonizing the bacterial wilt of eucalyptus, which comprises the pseudomonas SHP-38, the fermentation broth of the pseudomonas SHP-38 or the metabolite of the pseudomonas SHP-38. The invention also provides application of the pseudomonas SHP-38 or the microbial agent in inhibiting growth of bacterial wilt of eucalyptus. The invention also provides application of the pseudomonas SHP-38 or the microbial agent in preventing and treating plant bacterial wilt. The invention also provides application of the pseudomonas SHP-38 or the microbial agent in preparing products for preventing and treating plant bacterial wilt. The invention also provides application of the pseudomonas SHP-38 or the microbial agent in promoting plant growth. The invention also provides application of the pseudomonas SHP-38 or the microbial agent in preparing a plant growth promoter. Further, the plant includes eucalyptus. The invention also provides a product for preventing and treating plant bacterial wilt, which comprises the pseudomonas SHP-38 or the microbial agent. The invention discloses the following technical effects: The invention obtains the Pseudomonas (Pseudomonas sp.) SHP-38 with the effect of antagonizing the eucalyptus bacterial wilt, and confirms that the Pseudomonas SHP-38 belongs to a new species of Pseudomonas through physiological and biochemical identification and molecular classification identification. The bacterial strain antagonism experiment shows that the diameter of the antibacterial ring of the pseudomonas SHP-38 to the bacterial wilt of eucalyptus is not less than 30mm. Further analysis confirms that the pseudomonas SHP-38 has an inhibition effect on the formation of biological membranes of pathogenic bacterial strains and the flagella-mediated swimming capability, so that the colonization efficiency of pathogenic bacteria can be weakened, and the pathogenicity of the pathogenic bacte