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CN-122012340-A - Low-temperature-resistant Burkholderia G38, bacterial powder and preparation method and application thereof

CN122012340ACN 122012340 ACN122012340 ACN 122012340ACN-122012340-A

Abstract

The invention discloses low-temperature-resistant Burkholderia G38, bacterial powder and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of Burkholderia G38 is CCTCC NO: M2026181. The invention also provides a bacterial powder containing Burkholderia G38, which is prepared by culturing Burkholderia G38 to obtain a G38 bacterial liquid, centrifuging the G38 bacterial liquid to obtain bacterial mud, washing the bacterial mud, adding skimmed milk powder, uniformly mixing, freeze-drying, and grinding into powder. Tests prove that the G38 bacterial powder can still keep high-efficiency phosphorus dissolving activity under the low-temperature and low-rotation-speed culture condition, and based on the characteristics, corresponding fermentation and preparation processes can be developed, so that the dependence of a traditional method on high-energy-consumption stirring is eliminated, and the phosphorus dissolving preparation which is more suitable for actual soil environment and still is effective under the low-temperature condition is produced.

Inventors

  • WANG HEYUN
  • LU MINGXIN
  • WANG MIN

Assignees

  • 湖北工业大学

Dates

Publication Date
20260512
Application Date
20260319

Claims (10)

  1. 1. The low-temperature-resistant Burkholderia (Burkholderia pyrrocinia) G38 is characterized in that the Burkholderia G38 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M2026181 and the preservation address of university of Wuhan, china, and 01 month 20.
  2. 2. Use of burkholderia G38 according to claim 1 for the preparation of a bacterial powder.
  3. 3. A fungus powder is characterized in that, the bacterial powder comprises Burkholderia G38 according to claim 1.
  4. 4. A method of preparing the bacterial powder of claim 3, comprising the steps of: culturing Burkholderia G38 to obtain a G38 bacterial liquid, and centrifuging the G38 bacterial liquid to obtain bacterial mud; washing the bacterial mud, adding skimmed milk powder, mixing uniformly, freeze-drying, and grinding into powder to obtain the bacterial powder.
  5. 5. The method according to claim 4, wherein the culturing conditions are 120rpm and 30℃for 42 hours, and the centrifuging conditions are 3500rpm and 10 minutes; The volume ratio of the skimmed milk powder to the bacterial mud is 1:1.
  6. 6. The method according to claim 5, wherein the concentration of the skim milk powder is 10% to 20% by weight/volume.
  7. 7. The method according to claim 6, wherein the concentration of the skim milk powder is 10% by weight/volume.
  8. 8. Use of burkholderia G38 according to claim 1 or the bacterial powder according to claim 3 for dissolving phosphorus at low temperature and/or low rotational speed, wherein the low temperature comprises 10-20 ℃ and the low rotational speed comprises 0 rpm-50 rpm.
  9. 9. Use of burkholderia G38 according to claim 1 or the bacterial powder according to claim 3 for the preparation of a phosphorus-dissolving formulation for dissolving phosphorus at low temperatures and/or low rotational speeds; The low temperature comprises 10 ℃ to 20 ℃ and the low rotation speed comprises 0rpm to 50 rpm.
  10. 10. A method for dissolving phosphorus at low temperature and/or low rotational speed, comprising the step of adding burkholderia G38 according to claim 1 or the bacterial powder according to claim 3.

Description

Low-temperature-resistant Burkholderia G38, bacterial powder and preparation method and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to low-temperature-resistant Burkholderia G38, bacterial powder, a preparation method and application thereof. Background Phosphorus is an essential core nutrient element for crop growth and widely participates in key physiological processes such as photosynthesis, carbohydrate metabolism, energy transfer (ATP/ADP system), cell signal transduction and the like. However, phosphorus is very easy to combine with cations such as calcium, iron, aluminum and the like in soil to form insoluble phosphates which are difficult to be directly absorbed by plants, and the biological effectiveness is obviously reduced due to the strong holding effect. Although global soil total phosphorus content is typically between 400-1000 mg/kg, the concentration of available phosphorus (mainly in the form of HPO 42- and H 2PO4-) available to plants is extremely low, severely limiting the exploitation of crop production potential. The phosphorus deficiency of crops can cause a series of growth and development disorders, such as slow root system development, limited leaf extension, early aging and shedding, and blocked accumulation of assimilates in stems and leaves, finally resulting in reduced fruiting rate and remarkably reduced yield, and forming a direct threat to sustainable productivity of agriculture. In order to cope with the challenge, chemical phosphorus fertilizer is generally relied on and excessively added in the past decades of agricultural production, but the grain output is guaranteed in a short period of time, and various ecological environment problems such as soil nutrient imbalance, heavy metal and byproduct pollution, soil hardening acidification, water eutrophication, biodiversity reduction and the like are also caused, so that a path of sustainable development of agriculture faces a serious test. Therefore, development and popularization of an environmentally friendly technology for efficient utilization of phosphorus resources is urgent. Wherein, the functional microorganism (phosphorus-dissolving microorganism) capable of dissolving the indissolvable phosphate is utilized to prepare the indissolvable phosphate into a high-efficiency and stable microbial agent or biological fertilizer, and the microbial agent or biological fertilizer is regarded as one of the most potential solutions for fundamentally improving the biological effectiveness of the phosphorus in the soil, reducing the dependence of chemical phosphorus fertilizer and promoting the green transformation of agriculture. Phosphate-solubilizing bacteria (PSB) is a representative plant growth-promoting bacterium, and has the core function of participating in and promoting the soil-plant phosphorus circulation process. In the circulation process, phosphorus element is easily adsorbed by soil particles or combined with metal ions to form indissolvable phosphates such as iron-phosphorus (Fe-P), aluminum-phosphorus (Al-P), calcium-phosphorus (Ca-P) and the like, so that the bioavailability of the indissolvable phosphates is obviously reduced, and the indissolvable phosphates become one of main limiting nutrients for restricting plant growth. The insufficient supply of the soil phosphorus can obviously inhibit the normal growth and development of crops, and is characterized in that the formation and expansion of root systems are hindered, the photosynthesis efficiency of leaves is reduced, the capability of plants against biological stress such as disease and insect damage resistance and abiotic stress such as drought, saline alkali and the like is weakened, and finally the yield of crops is reduced. The core value of the phosphorus-dissolving bacteria is that the phosphorus in the soil in a indissolvable fixed state and the phosphorus in animal and plant residues can be converted into an available effective form of plants, and metabolic products such as auxin, organic acid, siderophores and the like can be secreted, so that the growth and development of crops are directly promoted, and an important support is provided for the safe production and stable supply of grains. The microorganism has double capacities of dissolving insoluble inorganic phosphorus and degrading organic phosphorus, and can be prepared into biological bacterial fertilizer to be applied to soil, so that the yield increase of crops can be realized by promoting the growth of plants. Currently, commercial phosphorus-dissolving bacteria agents have three general disadvantages. Firstly, the environmental suitability is poor, the activity is obviously reduced under the low-temperature (< 15 ℃) condition, and the application of the composition in early spring, late autumn and cold areas is limited. Secondly, the quality evaluation system is imperfect, the index of 'viable bacteria count' is seri