CN-122012342-A - Arthrobacter awamori for ammonia assimilation and application thereof in aquaculture water treatment

CN122012342ACN 122012342 ACN122012342 ACN 122012342ACN-122012342-A

Abstract

The invention discloses ammonia assimilating Arthrobacter and application thereof in aquaculture water treatment, relates to the technical field of wastewater treatment, and aims to provide ammonia assimilating Arthrobacter which is Arthrobacter (Arthrobacter arilaitensis) Aa01 and is preserved in China general microbiological culture collection center (CGMCC), wherein the preservation number is CGMCC No.36829, and the preservation date is 2025, 12 and 01. The application of the ammonia assimilating Arthrobacter arvensis in aquaculture water treatment. The ammonia assimilating Arthrobacter awamori Aa01 provided by the invention is a microorganism strain with obvious characteristics and application value, and the strain has the most obvious characteristics that the strain has a unique nitrogen conversion mechanism, can efficiently breathe NH 4 + -N、NO 2 ‑ -N and NO 3 ‑ -N serving as electron acceptors under aerobic conditions, breaks through the dependence of the traditional denitrification process on anaerobic environment, and shows excellent performance advantages compared with the conventional biological denitrification strain.

Inventors

QIN KENA
LIU XIAOYANG
Li Shuangyao
ZHU JIAQI
ZHANG DUOYING
ZHAO WENJUN

Assignees

黑龙江大学

Dates

Publication Date: 20260512
Application Date: 20260323

Claims (7)

1. The ammonia assimilating Arthrobacter is Arthrobacter (Arthrobacter arilaitensis) Aa01, and is preserved in China general microbiological culture collection center (CGMCC), with a preservation number of CGMCC No.36829 and a preservation date of 2025, 12 and 01.
2. The ammonia assimilating Arthrobacter apparatus according to claim 1, wherein Arthrobacter apparatus (Arthrobacter arilaitensis) Aa01 is cultured in a constant temperature shaking bed at a temperature of 120-160 r/min for 22-26 h at 28-32 ℃.
3. Use of the ammonia assimilating Arthrobacter arvensis of claim 1 for aquaculture water treatment.
4. Use according to claim 3, characterized in that the ammonia assimilating suspension of a. Albopictus bacteria is added to the aquaculture wastewater for treatment.
5. The use according to claim 3, wherein the OD600 value of the ammonia assimilating arthrobacter albopictus suspension is between 0.9 and 1.1.
6. Use according to claim 3, characterized in that the treatment is carried out with stirring or shaking at 30 ℃.
7. The use according to claim 3, wherein the ammonia assimilating Arthrobacter suspension is added in a proportion of 2% -3% by volume of the aquaculture wastewater.

Description

Arthrobacter awamori for ammonia assimilation and application thereof in aquaculture water treatment Technical Field The invention relates to the technical field of wastewater treatment, in particular to ammonia assimilating Arthrobacter arpisiformis and application thereof in aquaculture water treatment. Background In recent years, the aquaculture industry in China keeps a continuously growing situation, and the aquaculture industry has become one of core industries for guaranteeing grain safety and high-quality protein supply. According to statistical data, the total yield of aquatic products in China is 6648.87 ten thousand tons in 2025 and 1 to 11 months, and the yield is increased by 4.05% in a same ratio, wherein the mariculture yield is 2479.29 ten thousand tons, and the freshwater culture yield is 3179.95 ten thousand tons, and the 5.23% and 4.21% of the same ratio increase is respectively realized. Along with expansion of the cultivation scale and improvement of the intensification degree, the discharge amount of the cultivation wastewater is in a synchronous growth state, and the cultivation tail water treatment is required to be enhanced while the supply of the aquatic products is ensured. The aquaculture wastewater has the prominent problems of complex pollutant components, high nutrient salt concentration, large salinity fluctuation, antibiotic residues and the like, wherein the nitrogen pollution is particularly serious. The excessive accumulation of nitrogen in water not only damages ecological balance, endangers aquatic animal health, and may even pose a serious threat to human health, so denitrification from aquaculture wastewater has become a critical task to ensure high quality development of aquaculture industry. In the cultivation process, about 65% of nitrogen element in the feed is not effectively utilized by cultivated organisms, and enters a water body in a waste form to be converted into ammonia nitrogen. In addition, nitrite generated by ammonia nitrogen conversion can generate cancerogenic substances with ammonia compounds, and enter human bodies through consumption of aquatic products, so that health risks such as methemoglobin diseases are initiated, and meanwhile, nitrogen compounds can accelerate eutrophication of water bodies, induce algae outbreaks and further worsen the ecological environment of cultivation. The existing aquaculture wastewater treatment technology mainly comprises physical treatment, chemical treatment, biological treatment and a combined process, but has the obvious limitations that the physical treatment (such as filtration and precipitation) can only remove suspended particles, can not effectively degrade soluble ammonia nitrogen, has high operation cost, the chemical treatment (such as adding an oxidant and a flocculating agent) is easy to produce secondary pollution, the micro-ecological balance of a water body is destroyed, the drug residues of the aquatic products can be possibly exceeded, the traditional biological treatment technology (such as an artificial wetland and an anaerobic reactor) is obviously limited by environmental conditions such as hydraulic retention time, temperature and the like, the denitrification efficiency is unstable, and the adaptability to the aquaculture wastewater with high salt and antibiotic residues is poor. Most of the existing commercial microbial agents are nitrifying-denitrifying bacteria, the salinity and antibiotic tolerance of the existing commercial microbial agents to the culture environment are insufficient, and the existing commercial microbial agents are difficult to maintain effective activity in seawater culture or ponds subjected to drug control, so that the denitrification effect is greatly reduced. Therefore, the development of the efficient denitrification functional microbial inoculum suitable for the complex aquaculture environment becomes a key requirement for technical breakthrough of industry, ammonia assimilation bacteria with salt resistance and antibiotic resistance are screened, ammonia nitrogen is directly converted into biomass per se through the metabolism of the ammonia assimilation bacteria, the utilization of pollutant resources is realized, the secondary pollution risk of the traditional technology can be avoided, the extreme environment of high-density aquaculture can be adapted, the water pollution problem of aquaculture is solved, and the core break of the green transformation of industry is promoted. Disclosure of Invention The invention provides ammonia assimilating Arthrobacter arvensis and application thereof in aquaculture water treatment. The ammonia assimilating Arthrobacter is Arthrobacter arvensis (Arthrobacter arilaitensis) Aa01, and is preserved in China general microbiological culture collection center (CGMCC), the preservation address is the China national academy of sciences of China, no.3, north Star, the Korean area of Beijing, the preservation number is CGMCC No.36