CN-122012344-A - Burkholderia AnJ-129 with antagonism and growth promoting functions and application thereof

Abstract

The invention belongs to the technical field of biological control technology and microbiology, and relates to Burkholderia AnJ-129 with antagonistic and growth promoting functions and application thereof. The invention provides Burkholderia AnJ-129, and the preservation number of the Burkholderia AnJ-129 is CGMCC No.37457. The invention confirms that the burkholderia AnJ-129 belongs to a new species of burkholderia through physiological biochemical identification and molecular taxonomy identification, and expands species resources and genetic diversity of burkholderia flora. The burkholderia AnJ-129 provided by the invention can obviously antagonize pathogenic bacteria of the bacterial wilt of eucalyptus, and has a promoting effect on the growth of the eucalyptus. The invention develops a biological control and plant growth promoting method based on the strain, is used for controlling eucalyptus bacterial wilt caused by pathogenic bacteria, and has wide application prospect.

Inventors

• FAN XINLEI
• Gao Lanjing
• LI RUIFANG
• JIANG YUHAN
• ZHANG WEISHAN

Assignees

• 北京林业大学

Dates

Publication Date

20260512

Application Date

20260326

Claims (10)

1. 1. The Burkholderia (Burkholderia sp.) AnJ-129 is characterized in that the Burkholderia AnJ-129 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2026, 1 month and 19 days, a preservation number of CGMCC No.37457 and a preservation address of North Star Xili No.1, 3 in the Korean region of Beijing city.
2. 2. Use of burkholderia AnJ-129 as claimed in claim 1 for the preparation of a microbial agent for antagonising pathogenic bacteria of the bacterial wilt of eucalyptus.
3. 3. A microbial agent that antagonizes pathogenic bacteria of bacterial wilt of eucalyptus, wherein the microbial agent comprises burkholderia AnJ-129 of claim 1.
4. 4. Use of a microbial agent according to claims 1, to control bacterial wilt of plants, of burkholderia AnJ-129 or of claim 3.
5. 5. Use of a microbial agent according to claims 1, to the species burkholderia AnJ-129 or to claim 3 for the preparation of a product for controlling bacterial wilt of plants.
6. 6. Use of burkholderia AnJ-129 as claimed in claim 1 or a microbial agent as claimed in claim 3 for promoting plant growth.
7. 7. Use of a microbial agent according to claims 1, to the species burkholderia AnJ-129 or to claim 3, for the preparation of a plant growth promoter.
8. 8. The use according to any one of claims 4 to 7, wherein the plant comprises eucalyptus.
9. 9. A plant growth promoter is characterized in that, the plant growth promoter comprises the burkholderia AnJ-129 of claim 1 or the microbial agent of claim 3.
10. 10. A method of promoting plant growth comprising the step of administering the burkholderia substrate of claim 1 to the microbial agent of claim AnJ-129 or claim 3.

Description

Burkholderia AnJ-129 with antagonism and growth promoting functions and application thereof Technical Field The invention relates to the technical field of biological control technology and microbiology, in particular to Burkholderia AnJ-129 with antagonistic and growth promoting functions and application thereof. Background Eucalyptus is one of the important economic tree species. Along with the continuous expansion of the planting scale of artificial pure forests, eucalyptus diseases are also becoming serious. Bacterial wilt of eucalyptus is a major disease in eucalyptus production, and is serious and extremely destructive. The prevention and treatment of the eucalyptus bacterial wilt are mainly carried out by breeding disease-resistant clone and combining local chemical prevention and treatment. However, in the breeding process of the disease-resistant clone, along with the increase of the times of reproduction and subculture, the disease resistance of part of the disease-resistant clone is unstable or gradually reduced, thus preventing the sustainable development of the eucalyptus industry. The long-term use of chemical agents not only easily causes the drug resistance of pathogenic bacteria, but also may pollute the environment and jeopardize the balance of the ecological system. Therefore, the search for green, environmentally friendly and efficient control methods is a hotspot of current research. In addition, some microorganisms with the functions of nitrogen fixation, phosphorus dissolution, potassium dissolution and the like play an important role in microbial agents, and can effectively promote the circulation of nutrient elements in soil or secrete growth promoting hormone and antibiotic substances, so that the activity of plant growth is enhanced, the resistance of plant bodies to pathogen infection is improved, the occurrence of diseases is inhibited, and the aim of increasing yield is fulfilled. Therefore, the development of a green and safe biological control method by utilizing beneficial microorganisms with disease prevention and promotion effects has great significance for the sustainable development of the eucalyptus industry. Disclosure of Invention The invention aims to provide Burkholderia AnJ-129 with antagonistic and growth promoting functions and application thereof, so as to overcome the problems of plant disease resistance reduction and environmental pollution caused by chemical control. In order to achieve the above object, the present invention provides the following solutions: The invention provides a Burkholderia (Burkholderia) AnJ-129, wherein the Burkholderia AnJ-129 is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms), the preservation date is 2026, 1 month and 19 days, the preservation number is CGMCC No.37457, and the preservation address is North Star Xili No. 1, 3 in the Korean region of Beijing city. The invention provides application of Burkholderia AnJ-129 in preparing a microbial agent for antagonizing pathogenic bacteria of bacterial wilt of eucalyptus. The invention provides a microbial agent for antagonizing pathogenic bacteria of bacterial wilt of eucalyptus, which comprises the burkholderia AnJ-129. The invention provides application of Burkholderia AnJ-129 or the microbial agent in preventing and treating plant bacterial wilt. The invention provides application of Burkholderia AnJ-129 or a microbial agent in preparation of a product for preventing and treating plant bacterial wilt. The invention provides application of the Burkholderia AnJ-129 or the microbial agent in promoting plant growth. The invention provides application of Burkholderia AnJ-129 or a microbial agent in preparation of a plant growth promoter. Optionally, the plant comprises eucalyptus. The invention provides a plant growth promoter, which comprises the Burkholderia AnJ-129 or the microbial agent. Optionally, the plant comprises eucalyptus. Alternatively, the plant growth promoters may promote a significant increase in eucalyptus seedling height and root length. The invention provides a method for promoting plant growth, which is characterized by comprising the step of applying the Burkholderia AnJ-129 or the microbial agent. Optionally, the plant comprises eucalyptus. Alternatively, the plant growth is specifically to promote a significant increase in eucalyptus seedling height and root length. The invention discloses the following technical effects: The strain provided by the invention is an antagonistic strain with a growth promoting function, namely, burkholderia (Burkholderia) AnJ-129, is a new species of Burkholderia (Burkholderia), and expands species resources and genetic diversity of Burkholderia flora. The strain is obtained by separating and screening eucalyptus rhizosphere soil, and the bacterial colony on the LB culture medium is raised, round, light yellow to yellow and smooth and transparent in