CN-122012346-A - Pseudomonas fluorescens with benzo [ a ] anthracene degradation capability and application thereof

Abstract

The invention discloses Pseudomonas fluorescens with benzo [ a ] anthracene degradation capability and application thereof, and relates to the technical field of microbial degradation. The strain is Pseudomonas fluorescens (Pseudomonas fluorescens) SXU-ZW-01, and is preserved in China general microbiological culture Collection center (CGMCC) No.36511, and the preservation date is 2025, 11 and 05. The strain is separated from a coking plant environment sample, has typical high-temperature adaptation characteristics, can efficiently degrade benzo [ a ] anthracene under the condition of 45℃, pH, 7.0-8.0, and has a degradation rate of over 95 percent for benzo [ a ] anthracene for 96 hours. The invention provides core strain resources and technical support for green treatment of coking flue gas, and has good industrial application prospect.

Inventors

• ZHANG XIN
• WANG YIWEN

Assignees

• 山西大学

Dates

Publication Date

20260512

Application Date

20260327

Claims (10)

1. 1. The Pseudomonas fluorescens with benzo [ a ] anthracene degrading capability is characterized in that the Pseudomonas fluorescens (Pseudomonas fluorescens) SXU-ZW-01 is preserved in China general microbiological culture Collection center (CGMCC) No.36511, and the preservation date is 2025, 11 and 05.
2. 2. Use of a Pseudomonas fluorescens strain having benzo [ a ] anthracene degrading capacity according to claim 1 in environmental remediation.
3. 3. The use according to claim 2, characterized in that it is the use of pseudomonas fluorescens SXU-ZW-01 to degrade benzo [ a ] anthracene in an environment.
4. 4. The use according to claim 3, wherein the environment is at least one of coking fumes, coke oven fugitive gases, industrial waste gases, contaminated soil or contaminated water.
5. 5. A method for treating benzo [ a ] anthracene in coking flue gas by utilizing pseudomonas fluorescens according to claim 1, which is characterized by comprising the following steps of introducing coking flue gas containing benzo [ a ] anthracene into a biofiltration tower loaded with pseudomonas fluorescens SXU-ZW-01, and carrying out biodegradation reaction at 35-55 ℃ to realize removal of the benzo [ a ] anthracene.
6. 6. The method according to claim 5, wherein the temperature of the biodegradation reaction is 40-50 ℃ and the pH of the coked flue gas is 5.0-9.0.
7. 7. The method according to claim 5, characterized in that the empty tower residence time of the biofilter tower is 30-90s, preferably 45-60s.
8. 8. The method according to claim 5, wherein the temperature of the biodegradation reaction is 45 ℃, the pH of the coked flue gas is 7.0-8.0, and the empty tower residence time of the biofilter tower is 45-60s.
9. 9. A microbial agent for degrading benzo [ a ] anthracene, characterized in that the microbial agent contains pseudomonas fluorescens SXU-ZW-01 as an active ingredient.
10. 10. A biofiltration device, characterized in that the device comprises the microbial agent of claim 9, and a porous filler for supporting the microbial agent.

Description

Pseudomonas fluorescens with benzo [ a ] anthracene degradation capability and application thereof Technical Field The invention belongs to the technical field of microbial degradation, and particularly relates to pseudomonas fluorescens with benzo [ a ] anthracene degradation capability and application thereof. Background Benzo [ a ] anthracene is a typical tetracyclic polycyclic aromatic compound, listed as a class 2B carcinogen by the international cancer research institution, has strong teratogenicity, carcinogenicity and mutability, and constitutes a serious threat to the ecological environment and human health. The compound is mainly derived from the high-temperature cracking and incomplete combustion processes of organic matters such as coal, petroleum, wood and the like, and widely exists in industrial tail gas in the industries such as coking, steel, electric power, chemical industry and the like and waste gas discharged by motor vehicles. The polycyclic aromatic hydrocarbon pollutant has stable chemical properties, strong hydrophobicity and bioaccumulation, can remain in soil, water and atmospheric particulates for a long time after entering the environment, and finally endangers human health through enrichment and amplification of food chains, and pollution control becomes a key problem to be solved urgently in the field of environmental science. In the coking industry, the problem of emissions of benzo [ a ] anthracene is particularly pronounced. In the production process of the coke oven, various links such as coal charging, coke pushing, coke quenching, furnace top dissipation and the like can discharge a large amount of complex smoke containing benzo [ a ] anthracene. The smoke has extremely complex composition, contains polycyclic aromatic hydrocarbon pollutants, and often coexists with various components such as tar mist, dust particles, water vapor, sulfur dioxide, nitrogen oxides and the like. The coking flue gas temperature is generally 180-300 ℃, and the humidity is generally ‌ -18% ‌. At present, most of Taiyuan coking plants use a wet desulfurization technology to cool down to form low-temperature saturated wet flue gas, the temperature is 45-55 ℃, the dust and tar content is high, and a serious challenge is formed for the stable operation of a pollution control technology. The current emission standard of pollutants in coking chemical industry (GB 16171-2012) provides strict special emission limit requirements for characteristic pollutants such as benzo [ a ] anthracene, and coking enterprises are urgent to develop green high-efficiency treatment technology suitable for high-temperature, high-humidity and multi-pollutant working conditions. At present, physical-chemical methods are mainly adopted for treating polycyclic aromatic hydrocarbon pollutants such as benzo (a) anthracene and the like, and comprise the technologies of activated carbon adsorption, catalytic oxidation, wet washing, photocatalytic degradation and the like. However, these conventional techniques have a number of limitations when applied to coker flue gas remediation. Although the adsorption technology has good initial removal effect, the adsorbent is easy to saturate, difficult to regenerate and high in operation cost, and the saturated adsorbent needs to be further treated as dangerous waste. The catalytic oxidation technology has higher requirements on reaction conditions, tar and dust in coking flue gas easily cause poisoning and deactivation of a catalyst, and the problem of blocking a catalytic bed layer is serious. The wet washing technology can generate a large amount of oily and dusty washing wastewater, is easy to cause secondary pollution, and has the problems of equipment corrosion and filler blockage frequently, and has high operation and maintenance cost. The photocatalysis technology is influenced by factors such as poor light transmittance of the flue gas, high moisture content and the like, and is difficult to play a stabilizing effect in actual flue gas treatment. When the physical-chemical methods are used for treating complex working conditions such as coking flue gas and the like with high temperature, high humidity and coexisting multiple pollutants, the problems of poor operation stability, high energy consumption, high secondary pollution risk and the like are generally existed, and the increasingly strict environmental protection emission standard requirements are difficult to meet. The microbial degradation technology has become a research hot spot for deep treatment of polycyclic aromatic hydrocarbon due to the advantages of thorough mineralization, lower cost, mild operating conditions, less secondary pollution and the like. The technology utilizes the metabolism of microorganism to thoroughly mineralize organic pollutants such as benzo (a) anthracene into carbon dioxide and water, thus realizing the harmless treatment of the pollutants. In recent years, researchers at home and abroad s