CN-122012347-A - Bacillus cereus HUB0155 and application thereof

Abstract

The invention discloses bacillus cereus HUB0155 and application thereof. The strain is deposited with the Guangdong province microorganism strain collection (GDMCC) at 3/16 of 2026 under the deposit number GDMCC No:67964. The strain has good antagonism to 5 camellia oleifera pathogenic fungi, and the bacteriostasis rate reaches 44.67-70%. As endophytes of the camellia oleifera, HUB0155 is safer for camellia oleifera plants. Further inoculating the strain on the surface of an in-vivo camellia oleifera leaf infected by pathogenic bacteria, increasing the number of new leaves of the camellia oleifera plant treated by the bacterial liquid, obviously reducing fallen leaves and inhibiting the disease spots. Therefore, the invention provides a core strain resource for the development of green prevention and control technology of oil tea diseases, and has obvious development and application prospects in large-area biological prevention and control aspects of low-yield oil tea forest fallen leaves and fallen fruits.

Inventors

• LIU JIE
• ZHAO YUERONG
• WU YUTING
• Mao Lutian
• WANG CHUNLING
• XU LIANGXIONG

Assignees

• 华南农业大学
• 惠州学院

Dates

Publication Date

20260512

Application Date

20260327

Claims (10)

1. 1. A Bacillus cereus HUB0155 strain is characterized in that the strain is deposited in the microorganism strain collection (GDMCC) of Guangdong province on 3-month 16 of 2026, and the deposition number is GDMCC No:67964.
2. 2. A biological agent comprising a fermentation broth of bacillus cereus HUB0155 strain of claim 1 or bacillus cereus HUB0155 strain of claim 1.
3. 3. Use of bacillus cereus HUB0155 strain according to claim 1 or biological agent according to claim 2 for controlling pathogenic fungi of camellia oleifera.
4. 4. Use of bacillus cereus HUB0155 strain according to claim 1 or biological agent according to claim 2 for controlling diseases of camellia oleifera caused by pathogenic fungi of camellia oleifera.
5. 5. The use according to claim 3 or 4, wherein the camellia oleifera pathogenic fungus is a pathogenic fungus causing camellia oleifera fruit and leaf loss.
6. 6. The use according to claim 5, wherein said tea-oil camellia pathogenic fungi are selected from one or more of botrytis Neofusicoccum parvum, viticola Botryosphaeria dothidea, anthrax Colletotrichum camelliae or Colletotrichum gloeosporioides, amaranthus Neopestalotiopsis saprophytica.
7. 7. The use according to claim 4, wherein the oil tea disease is an oil tea fruit and leaf drop disease.
8. 8. Use of bacillus cereus HUB0155 strain according to claim 1 or biological agent according to claim 2 for promoting the growth of camellia oleifera.
9. 9. A method for preventing and controlling camellia oleifera from falling fruits and leaves, which is characterized in that camellia oleifera is inoculated with the bacillus cereus HUB0155 strain of claim 1 or the biological agent of claim 2.
10. 10. The method according to claim 9, wherein the inoculation method is foliar spraying, root irrigation or seed spraying and soaking.

Description

Bacillus cereus HUB0155 and application thereof Technical Field The invention relates to the technical field of agricultural microorganisms, in particular to bacillus cereus HUB0155 and application thereof. Background The Camellia oleifera (Camellia oleiferaAbel.) is a special woody oil crop in China, and the Camellia oleifera has high yield and high economic benefit. The oil tea not only has important economic value, but also plays an important role in ecological protection. However, the development of the oil tea industry is always limited by diseases, wherein the influence of fungal diseases is particularly prominent. There are 42 kinds of diseases of oil tea reported, wherein the fungal diseases account for 30 kinds, and the diseases mainly comprise oil tea anthracnose, oil tea soft rot, oil tea root rot and the like. These diseases commonly occur in various oil tea producing areas nationwide, the yield and quality of oil tea are seriously affected, the oil tea is dropped, the yield of the oil tea is reduced by 20-30%, and huge economic loss is caused. Aiming at the fungal diseases of oil tea, the traditional chemical pesticide control is a common method. But in recent years, the drawbacks of chemical pesticide control are increasingly revealed. In contrast, biological control has a series of advantages, and is regarded as an ideal choice for replacing or reducing the dosage of chemical pesticides due to the advantages of environmental friendliness, no residue, difficulty in generating drug resistance and the like. The core of the method is that antagonistic microorganisms or metabolites thereof are utilized to inhibit the infection of pathogenic bacteria from the source through mechanisms such as competition, parasitism, antibiotic and induction of plant resistance, and the like, so that the method has remarkable sustainable characteristic. In recent years, in the field of green prevention and control of oil tea diseases, the development of biocontrol bactericides has become a hot spot for biological control, can radically solve the problem of diseases, and simultaneously avoids the negative influence of chemical pesticides, thereby being a more sustainable control method. In the field of green prevention and control of oil tea diseases, research and development and application of biocontrol bactericides have become hot problems. The current researches on related biocontrol agents mainly surround the development of beneficial microorganisms such as trichoderma (such as trichoderma harzianum and trichoderma atroviride), bacillus (such as bacillus amyloliquefaciens and bacillus bailii) and pseudomonas, and target main diseases such as anthracnose, soft rot and root rot of oil tea. The technical evolution shows the trend of developing a single strain to a composite microbial inoculum, a simple preparation to a nano-carrier slow-release functional preparation and the like. Chinese patent application CN117821333A discloses a bacillus cereus capable of effectively preventing and treating strawberry leaf spot disease and receptacle calyx wilt, strawberry anthracnose, strawberry wilt and other fungal diseases caused by new Mucor pseudodisci (Neopestalotiopsis clavispora), anthracnose (Colletotrichum siamense) and fusarium oxysporum. However, the prevention and treatment effect of bacillus cereus on pathogenic fungi and fungal diseases of fallen leaves and fallen fruits, which cause direct loss of the yield of oil tea, is not disclosed. Disclosure of Invention The invention aims to overcome the defects and the shortcomings in the prior art and provide a Bacillus cereus HUB0155 strain. A second object of the present invention is to provide a biological agent. The third purpose of the invention is to provide application of the bacillus cereus HUB0155 strain or the biological agent in prevention and treatment of tea-oil camellia pathogenic fungi or tea-oil camellia diseases caused by the tea-oil camellia pathogenic fungi. The fourth purpose of the invention is to provide an application of the bacillus cereus HUB0155 strain or the biological agent in promoting the growth of tea-oil camellia. The fifth aim of the invention is to provide a method for preventing and controlling fallen leaves of oil tea fruits. The above object of the present invention is achieved by the following technical solutions: The invention provides a Bacillus cereus HUB0155 strain which is preserved in the microorganism strain preservation center (GDMCC) of Guangdong province at the 3 rd month and 16 th year of 2026, wherein the preservation number is GDMCC No:67964. The invention aims to clearly focus the aim of biological prevention and control of camellia oleifera on the prevention and control of camellia oleifera drop fruits and fallen leaf diseases, and separate and screen healthy camellia oleifera leaves collected from a dan Ma Zhen camellia oleifera planting base in the city of plum, guangdong to obtain Bacillus cereus HUB0155 with stab