CN-122012348-A - Bacillus strain and application thereof

Abstract

The invention provides a Bacillus strain, which is named as Bacillus sp and is preserved in the China general microbiological culture Collection center (CGMCC) No.37528, the preservation date is 2026 and the date is 26 days 01, and the 16S rRNA nucleotide sequence of the Bacillus sp is shown as SEQ ID NO. 1. Also provided are uses for promoting plant growth and improving plant quality. The Bacillus sp has the capabilities of phosphate dissolving, IAA secretion and iron carrier production, can promote plant growth and plant quality, and is used for promoting the growth of young wheat straw seedlings.

Inventors

• YUN LAN
• NARENTUYA
• JIA ZHENYU
• LI ZHEN
• WU JIERUI
• REN NANA
• WANG XUKAI
• ZHANG YUHAN

Assignees

• 内蒙古农业大学

Dates

Publication Date

20260512

Application Date

20260328

Claims (9)

1. 1. A Bacillus strain is named as Bacillus sp, and is preserved in China general microbiological culture Collection center (CGMCC) No.37528, and the preservation date is 2026, 01 and 26 days, and the 16S rRNA nucleotide sequence of the Bacillus sp is shown as SEQ ID NO. 1.
2. 2. Use of the bacillus strain of claim 1 for promoting plant growth and improving plant quality.
3. 3. The use according to claim 2, characterized in that the plant is neo-wheat straw.
4. 4. The use according to claim 3, wherein the bacillus strain is used to promote the growth of young wheat straw seedlings.
5. 5. The use according to claim 4, wherein the bacillus strain is used to promote plant height, root length, fresh weight above ground and fresh weight below ground of young wheat straw.
6. 6. The use according to claim 2, characterized in that the bacillus strain is prepared as a bacterial suspension.
7. 7. The use according to claim 6, wherein the bacterial suspension is prepared by the following method: Inoculating Bacillus sp into an LB liquid culture medium, shake culturing for 48h at 25 ℃ and 180rpm, and adjusting the concentration to OD 600 = 0.8 to obtain bacterial suspension.
8. 8. The use according to claim 7, wherein the method of application of the bacterial suspension is root irrigation.
9. 9. Use of a Bacillus strain according to claim 1, wherein the Bacillus sp has the ability to phosphate, secrete IAA and produce iron carriers.

Description

Bacillus strain and application thereof Technical Field The invention belongs to the technical field of bacillus, and particularly relates to a bacillus strain and application thereof. Background New wheat grass [ Psathyrostachys juncea (Fisch.) Nevski ] also called Russian wild rye (Russian wildrye) is a perennial cross pollinated plant of Gramineae, native to Siberia and middle asia, an excellent perennial pasture, and widely cultivated in North America major plain, russian, etc. The new wheat straw has strong stress resistance, developed root system, drought resistance, cold resistance, grazing resistance, barren resistance and salt and alkali resistance, long annual nutrition growth period, high nutrition value and strong regeneration, and is an ideal grass seed for establishing artificial grasslands and grazing grasslands. Is suitable for developing grassland animal husbandry, improving natural grassland and establishing artificial grassland, and is also an excellent rural grassland for ecological construction in northern cold and dry areas. Many students have studied new wheat plants from many aspects such as biology, physiology, breeding and cultivation, and particularly have attempted to analyze the stress resistance of the wheat plants from a genetic aspect. However, there is still a lack of research on root-soil interfaces of the plants directly related to stress resistance. Plant roots are the first barrier to stress and stress adaptation, and more studies indicate that the interaction between plant roots and specific microorganisms is specific, and thus rhizosphere microorganisms are also called "second genomes" of plants. The continuous growth and accumulation of root systems of perennial plants tend to form stable interaction relationship with microorganisms, so that the soil microenvironment and the plant growth and development are influenced for a long time. Grass seeds are preferred for ecological construction of the degenerated grasslands in the arid regions, and often need to survive and grow under poor environmental conditions, and the outstanding stress resistance is closely related to the action exerted by rhizosphere microorganisms, especially the growth promotion action of microorganisms in the seedling planting period. The slow growth of new wheat straw in seedling stage and the negative effect of environmental stress, how to establish the beneficial and stable root system-microbe interaction relationship as soon as possible are key jamming points for the early success of the grass planting. Therefore, the method screens specific pro-biotic microorganisms and utilizes the pro-biotic microorganisms to help the successful establishment of perennial grass seedlings, thereby having important value for ecological construction engineering and artificial grassland construction in cold and dry areas of China. Plant rhizosphere growth promoting bacteria (PGPR) act as a beneficial bacteria that colonizes around the plant root system, and can act directly or indirectly to promote plant growth. How to find key rhizosphere growth promoting bacteria and utilize the growth promoting effect of the rhizosphere growth promoting bacteria to promote the growth of new wheat straw in seedling stage is a key technical problem to be solved urgently at present. The present study collected and identified 33 rhizosphere bacteria from the rhizosphere of new wheat grass grown for many years at a number of different sites, from which strains with stronger physiological activity and significant growth promoting effect on new wheat grass seedlings were screened. So as to provide technical support for the efficient cultivation and management of new wheat straw. Disclosure of Invention The invention aims to solve the technical problems of the prior art, and provides a Bacillus strain and application thereof, wherein the Bacillus sp has the capabilities of phosphate dissolving, IAA secretion and siderophore production, can promote plant growth and plant quality, and is used for promoting the growth of new wheat straw seedlings. In order to solve the technical problems, the invention adopts the technical scheme that the Bacillus strain is named as Bacillus sp, is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center), has a preservation address of North Star Xiyu No. 1, baiyu No.3 in the Korean region of Beijing, china academy of sciences microbiological culture Collection center, has a preservation number of CGMCC No.37528, has a preservation date of 2026, 01 and 26 days, and has a 16S rRNA nucleotide sequence shown as SEQ ID NO. 1. The invention also provides application of the bacillus strain, which is used for promoting plant growth and improving plant quality. Preferably, the plant is neoryegrass. Preferably, the bacillus strain is used to promote the growth of young wheat seedlings. Preferably, the bacillus strain is used to promote plant he