CN-122012350-A - Lactobacillus plantarum P6 and static high-density culture method thereof

Abstract

The invention relates to the technical field of microorganisms, and provides a lactobacillus plantarum P6 and a static high-density culture method thereof, which realize the remarkable improvement of the density and activity of lactobacillus plantarum P6 by optimizing the coupling of a fermentation culture medium containing specific components and the sequential application of ultrasonic physical field stimulation in the logarithmic growth phase of the thallus under a static culture system without feed supplement and precise pH regulation, wherein the viable count of the thallus can reach The tolerance of the lactobacillus plantarum to digestive tract adversity such as bile salts is enhanced at the same time by more than CFU/mL, the low-cost and large-scale production requirement of the probiotic industry is met, and the bottleneck of the static culture viable count promotion of the traditional lactobacillus plantarum is broken through.

Inventors

• LUO JIE
• TAN LIANG
• FAN XIANKANG
• ZHOU HUI
• LI MAIQUAN
• YAN FANGFANG
• XIAO QIAN
• TANG XIA
• You Shanbing

Assignees

• 湖南农业大学

Dates

Publication Date

20260512

Application Date

20260401

Claims (10)

1. 1. The lactobacillus plantarum P6 is characterized in that lactobacillus plantarum (Lactiplantibacillus plantarum) P6 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for 24 days in 2025, the preservation address is North Star Xiyu No. 1, 3 in the Chaoyang area of Beijing, and the preservation number is CGMCC No. 36056.
2. 2. A static high-density culture method of lactobacillus plantarum P6 according to claim 1, characterized in that the static high-density culture method comprises the following steps: inoculating lactobacillus plantarum P6 into a fermentation medium for culturing at 37 ℃, wherein the fermentation medium comprises 5-15 g/L bagasse, 5-15 g/L beef extract powder, 1-5 g/L anhydrous sodium acetate and 40-80 mg/L histidine; applying ultrasonic physical field stimulation to the logarithmic growth phase of the thalli, and continuously culturing until the end to obtain the high-density thalli.
3. 3. The static high-density culture method according to claim 2, wherein the power of the ultrasonic physical field stimulation is 50-250W, the treatment time is 10-30 minutes, and the treatment temperature is 37 ℃.
4. 4. A static high density culture method according to claim 3, wherein the power of the ultrasonic physical field stimulation is 150W and the treatment time is 20 minutes.
5. 5. The method for culturing static high density according to claim 2, wherein the fermentation medium comprises 7.5 g/L bagasse, 12.5 g/L beef extract, 5 g/L anhydrous sodium acetate and 60 mg/L histidine.
6. 6. A lactobacillus plantarum P6 culture obtained by the culture method according to any one of claims 2 to 5, wherein the viable count of the culture is not less than CFU/mL, and survival rate after 4 hours treatment in 0.3% bile salt is not less than 80%.
7. 7. Use of lactobacillus plantarum P6 according to claim 1 for the preparation of a probiotic bacterial agent.
8. 8. Use of the lactobacillus plantarum P6 culture according to claim 6 for the preparation of a probiotic bacterial agent.
9. 9. Use of an ultrasound physical field for increasing the viable count of lactobacillus plantarum P6, bile salt tolerance and metabolite antioxidant and/or bacteriostatic activity according to claim 1.
10. 10. The use according to claim 9, wherein the power of the ultrasound physical field stimulation is 50-250W, the treatment time is 10-30 minutes, and the treatment temperature is 37 ℃.

Description

Lactobacillus plantarum P6 and static high-density culture method thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum P6 and a static high-density culture method thereof. Background Lactobacillus plantarum is one of the important probiotics, belongs to the genus Lactobacillus of the phylum Thick-walled bacteria, is gram-positive and converts sugars into lactic acid by homofermentation. In a host body, lactobacillus plantarum has multiple physiological functions of regulating intestinal flora, enhancing immune response, reducing serum cholesterol, maintaining intestinal barrier integrity and the like, and is widely applied to the fields of fermented foods, dietary supplements, medicines and health care. Along with the increasing demands of the market for high-activity probiotics preparations, the realization of high-density and low-cost industrialized culture of lactobacillus plantarum becomes an industrial key technical bottleneck. The core of realizing high-density culture is to overcome the nutrition limitation and metabolite inhibition in the culture process. High density culture is a key to improving the industrial efficiency of probiotics, and aims to obtain higher thallus density with shorter period and lower cost, thereby reducing the production cost and improving the competitiveness of the product. In the prior art, the optimization strategies are mainly divided into two categories, namely complex nutrient component optimization and feed supplement control, and the other category is to seek a simpler, more convenient and efficient culture mode. For example, the invention patent CN120349933a discloses a high-density culture method of lactobacillus plantarum, which optimizes the proportion of carbon source (trehalose), nitrogen source (beef extract) and manganese sulfate in a culture medium through an artificial neural network and a genetic algorithm, and adopts complex processes of dynamic fermentation, constant pH control and index feed supplement, so that higher thallus density is realized. However, when static culture is performed in its non-optimized MRS liquid medium, the number of living cells is onlyCFU/mL, the lifting space is huge. Although the method can obtain extremely high thallus density through a complex feed supplementing process, the method relies on precise fermentation tank equipment, continuous acid-base neutralization and complex feed supplementing calculation and control, and has the advantages of complex process, higher energy consumption, severe operation requirements, and unfavorable simplification and cost control of large-scale production. Another category of research has focused on developing static high density culture methods that are more economical in composition and simpler in process. The existing optimization means are mainly focused on the compounding of nutrient components in static culture, and although the existing optimization means have a certain effect, the existing optimization means cannot effectively relieve serious metabolic inhibition caused by lactic acid accumulation, the living bacteria number is limited to be increased, and the general breakthrough is difficultCFU/mL, and fail to effectively improve stress tolerance of the bacterial cells. Therefore, a novel lactobacillus plantarum culture method which has low raw material cost, simple process (such as static culture), can effectively relieve lactic acid inhibition, synchronously promote the cell density and the function tolerance of the lactobacillus plantarum is developed, and has important industrial requirements and application values. Disclosure of Invention In order to overcome the problems of the prior art, the invention provides a lactobacillus plantarum P6 strain, develops a static high-density culture method without complex dynamic feed supplement and precise pH control, realizes the remarkable improvement of the density and activity of lactobacillus plantarum P6 strain by optimizing the coupling of a fermentation culture medium containing specific components and the sequential application of ultrasonic physical field stimulation of the logarithmic growth phase of the strain, enhances the tolerance of the lactobacillus plantarum P6 strain to digestive tract stress such as bile salts and the like, and meets the requirements of low-cost and large-scale production of probiotic industry. The purpose of the invention is realized in the following way: The first aspect of the invention provides a lactobacillus plantarum P6, wherein the lactobacillus plantarum (Lactiplantibacillus plantarum) P6 is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for 24 days in 2025, the preservation address is North Star Xiyu No. 1, 3 in the Chaoyang area of Beijing, and the preservation number is CGMCC No. 36056. In a second aspect, the invention prov