CN-122012351-A - Pseudomonas aeruginosa and application thereof in river sewage denitrification

Abstract

The invention belongs to the field of microbiology, and relates to pseudomonas aeruginosa, which is named Pseudomonas aeruginosa in taxonomy and has a preservation number of CCTCC M2026385. The invention also provides a river sewage treatment method, which comprises the step of adding the pseudomonas aeruginosa into a river to carry out denitrification. The pseudomonas aeruginosa provided by the invention has denitrification performance under the condition of low carbon nitrogen ratio, and has higher denitrification capability in the environment of 4-20 carbon nitrogen ratio under the environment of 20-30 ℃, so that the pseudomonas aeruginosa provided by the invention can improve the water quality in river with low carbon nitrogen ratio.

Inventors

• PENG TAO
• Yang longteng
• LIU FANG
• LIANG GUOBIN
• LIN WEI
• XU YONG
• LI QIAN
• GUO YINGYING

Assignees

• 江苏理工学院
• 德恩特(江苏)环境科技有限公司

Dates

Publication Date

20260512

Application Date

20260401

Claims (9)

1. 1. Pseudomonas aeruginosa, taxonomic designation Pseudomonas aeruginosa, with accession number CCTCC M2026385.
2. 2. A microbial preparation comprising pseudomonas aeruginosa according to claim 1.
3. 3. The microbial preparation according to claim 2, wherein the content of pseudomonas aeruginosa in the microbial preparation is 1 x 10 6 CFU/mL or 1 x 10 6 CFU/g or more.
4. 4. A composition comprising the pseudomonas aeruginosa of claim 1 or the microbial agent of claim 2 or 3.
5. 5. A river sewage treatment agent, characterized in that it contains the pseudomonas aeruginosa of claim 1, the microbial preparation of claim 2 or 3 or the composition of claim 4.
6. 6. Use of the pseudomonas aeruginosa of claim 1, the microbial preparation of claim 2 or 3, the composition of claim 4 or the river sewage treatment agent of claim 5 for improving river water quality.
7. 7. The use according to claim 6, wherein the concentration of dissolved oxygen in the river water is lower than 1 mg/L.
8. 8. The use of claim 6, wherein the improvement in channel water quality comprises at least one of: (1) Reducing the chemical oxygen demand of river water; (2) Reducing the ammonia nitrogen content of river water; (3) Reducing the total nitrogen in river water.
9. 9. A method for treating river sewage, which is characterized in that pseudomonas aeruginosa according to claim 1, a microbial preparation according to claim 2 or 3, a composition according to claim 4 or a river sewage treating agent according to claim 5 are added into a river for denitrification.

Description

Pseudomonas aeruginosa and application thereof in river sewage denitrification Technical Field The invention belongs to the field of microbiology, relates to a strain and application thereof, and in particular relates to pseudomonas aeruginosa and application thereof. Background River channels are used as important collecting areas of urban surface runoff and pollutants, are easily influenced by various pollution sources such as domestic sewage, industrial wastewater and tail water discharge of sewage treatment plants, so that the water pollution problem is increasingly outstanding, and the aquatic ecological environment and the living production safety of residents are seriously threatened. Therefore, the development of river pollution control has important significance for improving river water quality, recovering water ecological functions and improving urban ecological environment quality. In the polluted urban river water body, the characteristics of higher ammonia nitrogen concentration, lower carbon nitrogen ratio (C/N) and the like are generally present. Ammonia nitrogen is one of important indexes of river water pollution, and excessive accumulation of ammonia nitrogen not only can cause black and odorous water and eutrophication phenomena, but also can cause serious influence on aquatic organism survival and stability of an aquatic ecological system. Therefore, reducing the ammonia nitrogen content in the river water body has become one of the important research directions of current river management. At present, biological denitrification technology is mainly adopted in the aspect of river sewage ammonia nitrogen treatment in China, and microbial strains with denitrification function are added into a water body to achieve biological conversion and removal of ammonia nitrogen. However, under the condition of low carbon nitrogen ratio which is commonly existed in urban river water, the growth activity and denitrification efficiency of the microorganism strain are often limited obviously, thereby influencing the actual treatment effect. Therefore, screening the microbial functional strain which can still have higher denitrification capability under the low carbon nitrogen ratio and low temperature environment conditions has important significance for improving the biological denitrification efficiency of the river water body and promoting the ecological restoration of the urban river. At present, the problem of low ammonia nitrogen treatment efficiency still exists in river sewage under the condition of low carbon nitrogen ratio. Disclosure of Invention Aiming at the technical problems in the prior art, the invention provides pseudomonas aeruginosa and application thereof, and the pseudomonas aeruginosa and application thereof solve the technical problem that the efficiency is low in treating ammonia nitrogen in river sewage in the prior art. The invention provides pseudomonas aeruginosa, which is named Pseudomonas aeruginosa in taxonomy and has a preservation number of CCTCC M2026385. The invention also provides a microbial preparation containing the pseudomonas aeruginosa. Further, the content of the pseudomonas aeruginosa in the microbial preparation is more than or equal to 1 multiplied by 10 6 CFU/mL or 1 multiplied by 10 6 CFU/g. The invention also provides a composition containing the pseudomonas aeruginosa or the microbial preparation. The invention also provides a river sewage treatment agent, which contains the pseudomonas aeruginosa, the microbial preparation or the composition. The invention also provides application of the pseudomonas aeruginosa, the microbial preparation, the composition or the river sewage treatment agent in improving the river water quality. Further, the concentration of the dissolved oxygen in the river water is lower than 1 mg/L. Further, the improvement of the river water quality at least comprises the following effects: (1) Reducing the Chemical Oxygen Demand (COD) of the river water; (2) Reducing the ammonia nitrogen content (NH 4 + -N) of river water; (3) Reducing Total Nitrogen (TN) in river water. The invention also provides a river sewage treatment method, which is to add the pseudomonas aeruginosa, the microbial preparation, the composition or the river sewage treatment agent into a river for denitrification. In one embodiment, the application is to inoculate a bacterial liquid cultured to an OD 600 of 0.5-1.5 into river sewage. In one embodiment, the bacterial liquid is inoculated into river sewage for reaction for at least 24 hours. In one embodiment, the inoculation amount of the bacterial liquid is 0.1-10%. In one embodiment, the use comprises water quality improvement. In one embodiment, the wastewater comprises river wastewater, aquaculture wastewater, livestock wastewater. The pseudomonas aeruginosa JSUTYLT001 provided by the invention has denitrification performance under the condition of low carbon nitrogen ratio. The pseudomonas aeruginosa JSUTYLT