CN-122012352-A - Lactobacillus gasseri for efficiently utilizing tryptophan to produce indole-3-lactic acid and application thereof

Abstract

The invention discloses a lactobacillus gasseri for producing indole-3-lactic acid by efficiently utilizing tryptophan and application thereof, and is characterized in that the strain is classified and named as lactobacillus gasseri Lactobacillus gasseri, the preservation number is CGMCC No.37546, the strain is metabolized to produce indole-3-lactic acid after anaerobic fermentation in a tryptophan-containing culture medium, the application of the lactobacillus gasseri in preparing constipation relieving products, emotional state regulating products and products for improving microecological balance of female genital tracts is provided, and the application of the strain in preparing pathogenic bacteria inhibitors and antioxidants is further provided; the strain can efficiently utilize tryptophan to metabolize and generate indole-3-lactic acid, and has an inhibition effect on escherichia coli, staphylococcus aureus and gardnerella vaginalis; can remove superoxide anions, DPPH free radicals and ABTS free radicals, and has good oxidation resistance.

Inventors

• ZHU JIAOLIN
• YANG CHENG
• TANG XIAOJING

Assignees

• 乾生(宁波)科技有限公司

Dates

Publication Date

20260512

Application Date

20260403

Claims (10)

1. 1. The lactobacillus gasseri for producing indole-3-lactic acid by using tryptophan is characterized in that the strain classification is named as lactobacillus gasseri (Lactobacillus gasseri), the strain QS766 has the preservation number of CGMCC No.37546, the preservation time is 2026 and 26 days in 01, the preservation unit is China general microbiological culture Collection center, the preservation address is North Star, no.1, 3 of the Korean area of Beijing, and the microbiological research institute of China academy of sciences.
2. 2. Use of a lactobacillus gasseri strain according to claim 1 for the production of indole-3-lactic acid, wherein the lactobacillus gasseri strain is metabolized to produce indole-3-lactic acid after anaerobic fermentation in a tryptophan-containing medium.
3. 3. The use according to claim 2, wherein the tryptophan-containing medium is an MRS liquid medium containing tryptophan at a final concentration of 1 to 3 g/L and 0.1 to 1wt% L cysteine hydrochloride.
4. 4. Use of the lactobacillus gasseri of claim 1 for the preparation of a product for relieving constipation.
5. 5. Use of lactobacillus gasseri as claimed in claim 1 in the manufacture of a product for modulating an emotional state.
6. 6. Use of the lactobacillus gasseri of claim 1 for the preparation of a product for improving microecological balance of the female genital tract.
7. 7. A microbial preparation comprising the lactobacillus gasseri of claim 1, wherein the microbial preparation is selected from the group consisting of lactobacillus gasseri bacterial suspension, fermentation supernatant, lyophilized bacterial powder, and combinations thereof.
8. 8. Use of a lactobacillus gasseri according to claim 1 or a microbial preparation according to claim 7 for the preparation of a pathogenic bacteria inhibitor, wherein the pathogenic bacteria is selected from at least one of staphylococcus aureus, escherichia coli and gardnerella vaginalis.
9. 9. Use of the lactobacillus gasseri of claim 1 or the microbial preparation of claim 7 for the preparation of an antioxidant.
10. 10. The use according to claim 9, wherein the antioxidant is a superoxide anion scavenger, ABTS radical scavenger and/or DPPH radical scavenger.

Description

Lactobacillus gasseri for efficiently utilizing tryptophan to produce indole-3-lactic acid and application thereof Technical Field The invention relates to lactobacillus gasseri, in particular to lactobacillus gasseri for producing indole-3-lactic acid by efficiently utilizing tryptophan and application thereof. Background Indole derivatives are a natural product produced by the metabolism of tryptophan, and indole-3-lactic acid belongs to one of indole derivatives. The substance is an endogenous ligand of aromatic hydrocarbon receptor (AhR), has anti-inflammatory effect, and has non-negligible effect in relieving constipation and inflammatory bowel disease, and regulating emotion. The prior research results show that indole-3-lactic acid can act on human intestinal tracts as a ligand of an aromatic hydrocarbon receptor (AhR). AhR can regulate intestinal mucosa immune homeostasis and epithelial barrier gene after activation, promote expression of tight junction protein, promote repair of intestinal epithelial cells, reduce intestinal permeability, inhibit NF- κB pathway, downregulate inflammatory factors, reduce intestinal inflammation, and relieve constipation and inflammatory bowel disease. Besides, after AhR is activated, the neuroinflammation can be obviously inhibited, and the level of pro-inflammatory factors in the brain can be reduced, so that neurons in brain areas such as hippocampus and the like can be protected, the neurogenesis inhibition caused by chronic stress can be improved, and depression/anxiety-like behaviors can be improved. Lactobacillus gasseri (Lactobacillus gasser) is considered to be an effective probiotic for the prevention and treatment of vaginal infections. The vaginal mask can produce lactic acid by fermenting saccharides, reduce the pH value in the vagina, has stronger antibacterial capability by secreting antibacterial substances (such as bacteriocin and the like), can effectively inhibit the growth of pathogenic bacteria, and reduces the occurrence risk of vaginal infection. It can inhibit the growth of pathogenic microorganisms by improving the micro-ecological environment of vagina, and reduce the incidence rate of common vaginal infection such as bacterial vaginosis, colpitis mycotica and the like. At present, related research reports of lactobacillus gasseri for producing indole-3-lactic acid by utilizing tryptophan are not seen on the market. Disclosure of Invention The invention aims to provide lactobacillus gasseri for producing indole-3-lactic acid by efficiently utilizing tryptophan and application thereof. The technical scheme adopted by the invention for solving the technical problems is that the lactobacillus gasseri for efficiently utilizing tryptophan to produce indole-3-lactic acid is classified and named as lactobacillus gasseri (Lactobacillus gasseri), QS766 strain, the preservation number is CGMCC No.37546, the preservation time is 2026, the month 26, the preservation unit is China general microbiological center, the preservation address is North Star, the national institute of microbiology, the university of China, the International Association of China, the Korean area North Star, the national institute of microbiology, and the preservation address is North Star, the national institute of microbiology. The invention also provides application of the lactobacillus gasseri strain in indole-3-lactic acid production, wherein the lactobacillus gasseri strain is metabolized to produce indole-3-lactic acid after anaerobic fermentation in a tryptophan-containing culture medium. Further, the tryptophan-containing medium is MRS liquid medium containing 1-3 g/L tryptophan and 0.1-1wt% L-cysteine hydrochloride. The invention also provides application of the lactobacillus gasseri in preparing a constipation relieving product. The invention also provides application of the lactobacillus gasseri in preparing a product for regulating emotional state. The invention also provides application of the lactobacillus gasseri in preparing a product for improving microecological balance of female genital tracts. The invention also provides a microbial preparation containing the lactobacillus gasseri, wherein the microbial preparation is selected from bacterial suspension, fermentation supernatant, freeze-dried bacterial powder or a combination thereof. The invention also provides application of the lactobacillus gasseri or the microbial preparation in preparation of a pathogen inhibitor, wherein the pathogen is at least one selected from staphylococcus aureus (Staphylococcus aureus), escherichia coli (ESCHERICHIA COLI) and gardnerella vaginalis (GARDNERELLA VAGINALIS). The invention also provides application of the lactobacillus gasseri or the microbial preparation in preparing an antioxidant. Further, the antioxidant comprises a superoxide anion scavenger, an ABTS radical scavenger and/or a DPPH radical scavenger. Compared with the prior art, the lactobacillus gasseri for effic