CN-122012353-A - Streptomyces thermophilus carbon monoxide and composite microbial inoculum thereof, preparation method and application

Abstract

A thermophilic carbon monoxide streptomycete, a composite microbial agent, a preparation method and application thereof belong to the technical field of microorganisms, the strain classification is named as thermophilic carbon monoxide streptomycete (Streptomyces thermocarboxydus), the strain is named as G-4-7, the preservation number is CGMCC No.36417, the thermophilic carbon monoxide streptomycete, the composite microbial agent and soil bacillus brevis (Brevibacillus agri) G-5-2 and chaetomium thermophilum (Chaetomium thermophilum) are used for preparing the efficient lignin degradation composite microbial agent, the composite microbial agent can degrade bagasse lignin in a high-temperature environment efficiently, enzyme systems of all strains are complementary, degradation efficiency is synergistically improved, resource utilization of bagasse rich in lignin can be promoted, and a technical basis is provided for a green treatment mode of agricultural wastes.

Inventors

• ZHOU HAO
• Xie Lianglong
• ZHU YONGWEI
• LI JIANBIN
• ZHU BIYANG
• LI KAI
• DENG LIGAO
• He Huiban
• LIANG HANZHU
• DING DE
• HUANG ZHI

Assignees

• 广西大学

Dates

Publication Date

20260512

Application Date

20260410

Claims (6)

1. 1. The thermophilic streptomyces carbon monoxide is characterized in that the strain classification is named as thermophilic streptomyces carbon monoxide (Streptomyces thermocarboxydus), the strain is named as G-4-7, the preservation number is CGMCC No.36417, the preservation organization is China general microbiological culture Collection center, and the preservation date is 2025, 10 months and 30 days.
2. 2. The use of the thermophilic streptomyces carbon monoxide according to claim 1 for the preparation of a complex microbial inoculant promoting lignin degradation.
3. 3. The use of a thermophilic Streptomyces carbon monoxide as defined in claim 1 for the preparation of a biological agent producing a lignin enzyme comprising one or more of laccase Lac, lignin peroxidase LiP and manganese peroxidase MnP.
4. 4. A composite microbial inoculum is characterized in that the composite microbial inoculum is prepared from thermophilic carbon monoxide streptomyces (Streptomyces thermocarboxydus) G-4-7, soil bacillus brevis (Brevibacillus agri) G-5-2 and thermophilic chaetomium (Chaetomium thermophilum); the thermophilic streptomyces carbon monoxide (Streptomyces thermocarboxydus) G-4-7 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 36417 in the 10 th month of 2025; the Brevibacillus tumefaciens (Brevibacillus agri) G-5-2 is preserved in China general microbiological culture collection center (CGMCC) with a preservation number of 36418 in the 10 th month and 30 th year of 2025; The chaetomium thermophilum (Chaetomium thermophilum) is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC 3.17990.
5. 5. The composite microbial inoculum according to claim 4, wherein the preparation method comprises the steps of inoculating thermophilic carbon monoxide streptomycete (Streptomyces thermocarboxydus) G-4-7, soil bacillus brevis (Brevibacillus agri) G-5-2 and thermophilic chaetomium (Chaetomium thermophilum) into a liquid culture medium respectively, culturing at 50 ℃ and adjusting the colony quantity to 1X 10 8 CFU/mL, and mixing the strains in equal volume to obtain the composite microbial inoculum.
6. 6. The use of the composite microbial inoculant of claim 4 for degrading sugar cane sugar production waste.

Description

Streptomyces thermophilus carbon monoxide and composite microbial inoculum thereof, preparation method and application Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a thermophilic streptomyces carbon monoxide and a composite microbial inoculum thereof, a preparation method and application. Background Economic development and population growth have driven the expansion of agricultural production scale, thereby producing a large amount of agricultural waste. The sugarcane is the sugar crop with the largest planting area in the world, is also the most important energy source and feed crop, and has higher ecological and economic values. Following Brazil and India, china is the third largest sugarcane producing country worldwide. Guangxi is the largest area sugarcane area in China, and in recent years, 5 million tons of sugarcane are produced on average, and 280 kg of bagasse is produced after each ton of sugarcane is processed. Approximately 1.81 million tons of bagasse are produced annually worldwide, with the major component being lignocellulose. Most of the bagasse is discarded or burned. If the agricultural wastes are not properly treated, the problems of resource waste and environmental pollution are caused. Aerobic composting of solid wastes rich in lignocellulose tends to show a lower degradation efficiency due to the difficult degradability of lignocellulose, in particular the lignin component. In order to promote degradation of lignocellulose and shorten the composting process, composting is usually carried out by adding an exogenous microbial agent. The high temperature period of the compost is a key stage of biological decomposition of organic matters and harmless treatment of the compost, but microorganisms are often influenced by high temperature factors at the stage, population quantity and activity are generally reduced, and the residence time of the high temperature period is short, so that the rapid decomposition of the organic matters is limited. Thermophilic streptomyces carbon monoxide (Streptomyces thermocarboxydus) belongs to the phylum actinomycetes, the family Streptomycetaceae. The most suitable growth temperature is usually 50-65 ℃ for thermophilic bacteria, and the enzymes and metabolic systems contained in the body have natural thermal stability. Making it an ideal candidate for developing biomass conversion technology and realizing waste resource utilization, and providing a new solution for sustainable circular economy and biological manufacture. The prior literature records, for example, 1 and Chinese patent, a new strain of streptomyces thermophilus and application thereof, publication No. CN103484396A, the new strain CF1 can rapidly raise the temperature of a stack body in northern low-temperature seasons, can keep the high temperature of the stack body above 50 ℃ for 15 days, shortens the fermentation period, 2 and Chinese patent, a strain of streptomyces thermophilus low-temperature subspecies Dstr3-3 and application thereof, publication No. CN104988095A, and the strain Dstr3-3 can produce lignin peroxidase and manganese peroxidase without laccase. 3. Chinese patent, a strain of high temperature resistant garden waste decomposing bacteria ST3 and application thereof, publication number of CN105567607A, wherein the thermophilic streptomyces carbon monoxide (Streptomyces thermocarboxydus) ST3 is a strain for degrading cellulose screened from garden waste compost, more cellulase can be produced within the temperature range of 40-70 ℃, and the enzyme activity is as high as 30.05U/mL. 4. Chinese patent, a method for producing cellulase by using thermophilic streptomyces carbon monoxide and a liquid fermentation culture medium, wherein the publication number is CN106191012A, and the concentration of the cellulase produced by using thermophilic streptomyces carbon monoxide reaches 0.80IU/mL. Although some prior art documents have been directed to the secretion of lignocellulose enzymes by S.thermophilus, the specific ability of enzymes to degrade lignin has not been analyzed or studied in detail. On the other hand, while the use of complex microbial systems to degrade lignocellulose by synergism has been considered a more efficient strategy than single strains, there are significant drawbacks in the prior art. First, many microbial agents are not specifically optimized for the component characteristics of bagasse, and have strong universality and poor specificity. Secondly, the conventional composite microbial inoculum lacks high temperature resistance and cannot continuously play a role in the high temperature period of fermentation. In addition, the existing artificial compounding mode is often only simple combination of strains, and metabolic complementarity and synergistic stability of specific substrates, namely bagasse, among the strains under high temperature conditions cannot be fully considered. Therefore, the nat