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CN-122012364-A - Enrichment and culture method of polyethylene glycol degradation flora

CN122012364ACN 122012364 ACN122012364 ACN 122012364ACN-122012364-A

Abstract

The invention relates to the technical field of enrichment and culture of polyethylene glycol degradation bacteria, and discloses an enrichment and culture method of polyethylene glycol degradation bacteria, which comprises the following steps that in the first stage, PEG100 and PEG800 are used as substrates in water, the COD concentration is controlled at 1000mg/L and 3000mg/L, and is alternately changed according to the period, The concentration is controlled to be 20-40mg/L, in the second stage, PEG100, PEG800 and PEG1000 are used as substrates, the COD concentration is controlled to be 3000mg/L and 5000mg/L, and is changed alternately according to the period, The third stage, in which PEG100, PEG800, PEG1000 and PEG4000 are used as substrates, the COD concentration is controlled at 5000mg/L and 7000mg/L, and the Chemical Oxygen Demand (COD) concentration is alternately changed according to the period, The concentration is controlled to be 60-80mg/L, in the fourth stage, PEG100, PEG800, PEG1000, PEG4000 and PEG6000 are used as substrates, the COD concentration is controlled to be 7000mg/L and 9000mg/L, and the Chemical Oxygen Demand (COD) concentration is alternately changed according to the period, The concentration is controlled to be 80-100mg/L, and the bacterial colony contains specific degradation bacteria aiming at various PEG molecular weights through enrichment culture at four stages, so that the bacterial colony has strong tolerance to polyethylene glycol concentration and molecular weight fluctuation.

Inventors

  • CHEN MAOXIA
  • RU JING
  • YI YAN
  • SONG LIHONG
  • LI YANG

Assignees

  • 乐山师范学院

Dates

Publication Date
20260512
Application Date
20260209

Claims (8)

  1. 1. The enrichment and culture method of polyethylene glycol degrading flora is characterized by comprising the following steps: In the first stage, PEG100 and PEG800 are used as substrates in the inflow water, the COD concentration is controlled at 1000mg/L and 3000mg/L, and is alternately changed according to the period, The concentration is controlled at 20-40mg/L; Step two, in the second stage, PEG100, PEG800 and PEG1000 are used as substrates in water inflow, the COD concentration is controlled at 3000mg/L and 5000mg/L, and is alternately changed according to the period, The concentration is controlled at 40-60mg/L; step three, in the third stage, PEG100, PEG800, PEG1000 and PEG4000 are used as substrates in the inflow water, the COD concentration is controlled at 5000mg/L and 7000mg/L, and is changed alternately according to the period, The concentration is controlled to be 60-80mg/L; In the fourth stage, PEG100, PEG800, PEG1000, PEG4000 and PEG6000 are used as substrates in the water, the COD concentration is controlled at 7000mg/L and 9000mg/L, and the Chemical Oxygen Demand (COD) concentration is alternately changed according to the period, The concentration is controlled to be 80-100mg/L.
  2. 2. The method of claim 1, wherein the polyethylene glycol of the plurality of molecular weights in each stage provides an equivalent amount of COD, and wherein the polyethylene glycol substrate load between the two stages alternates.
  3. 3. The method of claim 1, wherein the polyethylene glycol substrate load during each stage of enrichment is increased and the molecular weight of the polyethylene glycol used is increased.
  4. 4. The method of claim 1, wherein the first stage of the first step is 1-6 cycles.
  5. 5. The method of claim 4, wherein the second stage in the second step is 7-11 cycles.
  6. 6. The method of claim 5, wherein the third stage in the third step is 12-17 cycles.
  7. 7. The method of claim 6, wherein the fourth stage in the fourth step is 18-30 cycles.
  8. 8. The method of claim 7, wherein the period of time for one cycle is 24 hours.

Description

Enrichment and culture method of polyethylene glycol degradation flora Technical Field The invention relates to the technical field of enrichment and culture of polyethylene glycol degradation flora, in particular to an enrichment and culture method of polyethylene glycol degradation flora. Background Polyethylene glycol (Polyethylene Glycol, PEG) is a water-soluble high polymer polymerized by ethylene oxide, the chemical formula is HO (CH 2CH2O)n H, which is widely used as a solvent in the industries of medicine, cosmetics, textile, chemical industry, new energy sources and the like, but the degradation-resistant characteristic of the polyethylene glycol causes accumulation of polyethylene glycol in industrial wastewater and potential hazard to the environment, polyethylene glycol can be classified into PEG100, PEG200, PEG400, PEG1000 and PEG4000 according to molecular weight, even the molecular weight is higher than 20000, and the yield of the polyethylene glycol exceeds 100 ten thousand tons every year according to statistics, the traditional physical and chemical treatment methods such as membrane filtration, activated carbon adsorption, iron-carbon micro-electrolysis oxidation, advanced oxidation, fenton oxidation and membrane separation technology, although the method can be partially removed, the operation is simple, but the problems of incomplete treatment, high cost, high energy consumption, easy secondary pollution and the like exist. However, the current activated sludge process for treating polyethylene glycol wastewater has the following problems that (1) bacterial groups in common activated sludge can remove conventional organic pollutants and have no pertinence to polyethylene glycol degradation, (2) common activated sludge has limited effect on high-concentration polyethylene glycol wastewater treatment, (3) the effect on high-molecular-weight polyethylene glycol treatment is poor, (4) polyethylene glycol concentration and molecular weight category fluctuation are large, so that the treatment effect is unstable, and (5) in the existing literature, the method for enriching polyethylene glycol degrading bacteria has single molecular weight category of polyethylene glycol, and the obtained degrading bacteria have poor effect on polyethylene glycol wastewater treatment of other molecular weights. Therefore, in order to solve the technical problems, an enrichment and culture method of polyethylene glycol degrading flora is provided. Disclosure of Invention The invention provides an enrichment and culture method of polyethylene glycol degradation flora, which enables the obtained polyethylene glycol degradation flora to adapt to polyethylene glycol wastewater with COD concentration of 1000mg/L to 9000mg/L and PEG100 to PEG6000 molecular weight through enrichment culture of four stages, and has stronger tolerance to polyethylene glycol concentration and molecular weight fluctuation due to the inclusion of specific degradation bacteria aiming at various PEG molecular weights in the flora. The invention provides the following technical scheme: A method for enriching and culturing polyethylene glycol degradation bacteria includes such steps as preparing PEG100 and PEG800 as substrate in water, controlling COD concentration at 1000mg/L and 3000mg/L, periodically and alternatively changing, The concentration is controlled to be 20-40mg/L, and in the second stage, PEG100, PEG800 and PEG1000 are used as substrates in water inflow, COD concentration is controlled to be 3000mg/L and 5000mg/L, and is alternately changed according to the period,The third stage, in which PEG100, PEG800, PEG1000 and PEG4000 are used as substrates in the water, the COD concentration is controlled at 5000mg/L and 7000mg/L, and the concentration is alternately changed according to the period,The fourth stage, in which PEG100, PEG800, PEG1000, PEG4000 and PEG6000 are used as substrates, the COD concentration is controlled at 7000mg/L and 9000mg/L, and the Chemical Oxygen Demand (COD) is alternately changed according to the period,The concentration is controlled to be 80-100mg/L. As a preferred embodiment of the invention, the polyethylene glycol of various molecular weights in each stage provides an equivalent amount of COD, with alternating polyethylene glycol substrate loading between the two stages. As a preferred embodiment of the present invention, the polyethylene glycol substrate load during the enrichment of each stage is increased and the polyethylene glycol molecular weight used is increased. As a preferable technical scheme of the invention, the first stage in the first step is 1-6 cycles. As a preferable technical scheme of the invention, the second stage in the step two is 7-11 cycles. As a preferable technical scheme of the invention, the third stage in the step three is 12-17 cycles. As a preferable technical scheme of the invention, the fourth stage in the step four is 18-30 cycles. As a preferred embodiment of the