CN-122012380-A - Application of metabolic small molecule additive in promoting bovine in-vitro embryo development process

CN122012380ACN 122012380 ACN122012380 ACN 122012380ACN-122012380-A

Abstract

The invention provides an application of a metabolic small molecule additive in promoting the development process of bovine in-vitro embryos, belonging to the technical field of in-vitro embryo production. In particular to an application of polypeptide substance N-acetyl aspartic acid-glutamine (N-acetylaspartylglutamine, NAAG) in promoting blastula formation in the bovine embryo culture process in vitro. The metabolic analysis and experimental results prove that NAAG is added into an in vitro embryo culture system, so that the blastocyst formation rate of the bovine in vitro fertilized embryo can be obviously improved, and the in vitro embryo development efficiency and quality can be improved. The invention provides a new technical means for optimizing the in-vitro embryo culture system of cattle, improving embryo breeding efficiency and promoting the rapid propagation of improved cattle seeds, and has important application value and popularization significance in the fields of livestock genetic breeding and animal embryo engineering.

Inventors

ZHANG KUN
SHENG HAO
WANG HUANAN

Assignees

浙江大学

Dates

Publication Date: 20260512
Application Date: 20260114

Claims (6)

1. The application of the metabolic small molecule additive in promoting the development process of bovine in-vitro embryos is characterized in that the metabolic small molecule additive is N-acetyl aspartic acid-glutamine.
2. The use according to claim 1, characterized in that the metabolic small molecule additive is added in the form of droplets to an in vitro maturation medium for culturing bovine in vitro embryos.
3. The use according to claim 2, wherein the final concentration of metabolic small molecule additives in the in vitro maturation medium is 1 μm.
4. The use according to claim 2, wherein the metabolic small molecule additive is added at a time point when the fertilized bovine in vitro embryo egg starts to be cultured in vitro.
5. The use according to any one of claims 1 to 4, wherein said promotion of bovine in vitro embryo development is manifested by an increase in the rate of blastocyst formation following in vitro fertilization in cattle.
6. A culture medium composition for promoting bovine in vitro embryo development, comprising in vitro maturation medium and N-acetyl aspartic acid-glutamine at a final concentration of 1 μm in the medium.

Description

Application of metabolic small molecule additive in promoting bovine in-vitro embryo development process Technical Field The invention belongs to the technical field of embryo in-vitro production, and particularly relates to application of a metabolic small molecule additive in the process of promoting bovine in-vitro embryo development. Background In recent years, with the increasing demand of the milk industry in China for high-quality germplasm resources, in vitro embryo production (In Vitro Production, IVP) technology gradually becomes an important auxiliary means for cow breeding. Compared with the traditional in-vivo embryo transfer, the IVP technology is not limited by the estrus cycle of a donor, can be carried out all year round, can obviously improve the reproduction efficiency and shorten the breeding cycle, and therefore has important application value in the genetic improvement of dairy cows in a national range. However, the current bovine embryo IVP system in China still faces a great challenge. Although the in vitro fertilization rate is relatively stable, the blastula formation rate is generally low, which is significantly lower than the development efficiency of in vivo embryos. The blastula has unstable quality, low survival rate after freezing and storage, and is a main factor for restricting the large-scale popularization of the technology. The key to these problems is that in vitro culture environments are difficult to completely simulate the microenvironment in the mother uterus and oviduct, embryos are easily limited in terms of metabolism, oxidative stress regulation and nutrient utilization, and thus the normal development potential of embryos is affected. Under the background, how to improve the blastula formation rate and blastula quality by optimizing the culture system and improving the components of the culture medium becomes a core problem of domestic and foreign researches. Embryo development quality is closely related to the metabolite environment in the culture medium. In order to meet the embryo development requirement, exogenous substances are usually required to be supplemented in a culture medium, and amino acid substances are also a common additive. N-acetyl aspartic acid-glutamine (NAAG) is a neuropeptide that is highly abundant in the mammalian central nervous system. No report on the application of NAAG in embryo development or culture systems is found in the current published materials. Disclosure of Invention The invention aims to solve the problems of low blastula formation rate and poor quality in the existing bovine in-vitro embryo production process, and provides an application of a metabolic small molecule additive in the bovine in-vitro embryo development promotion process, wherein the metabolic small molecule additive N-acetyl aspartic acid-glutamine (NAAG) is safe, stable and easy to apply. The invention provides application of a metabolic small molecule additive in promoting bovine in-vitro embryo development, wherein the metabolic small molecule additive is N-acetyl aspartic acid-glutamine. The molecular structural formula of the additive is shown as (I): Further, the application is specifically that the metabolic small molecule additive is added into an in vitro maturation medium for culturing bovine in vitro embryos by liquid drop. Further, the final concentration of the metabolic small molecule additive in the in vitro maturation medium is 1. Mu.M. Further, the adding time point of the metabolism small molecule additive is when the fertilized ovum of the bovine in vitro embryo starts to culture in vitro. Further, the promotion of bovine in vitro embryo development is reflected in improving the blastocyst formation rate of the cattle after in vitro fertilization. The invention also provides a culture medium composition for promoting bovine in-vitro embryo development, which comprises an in-vitro maturation culture medium and N-acetyl aspartic acid-glutamine with a final concentration of 1 mu M in the culture medium. The N-acetyl aspartic acid-glutamine provided by the invention has the beneficial effects that the N-acetyl aspartic acid-glutamine provided by the invention can obviously improve the blastula formation rate of bovine in-vitro fertilized embryos, and the effect is clear. Experiments prove that by adding NAGG into the bovine embryo in-vitro culture system, the final concentration is 1 mu M, the blastula formation rate of the bovine in-vitro fertilized embryo can be obviously improved from about 27% to about 43%, and the blastula rate is improved by about 15%, so that NAAG has obvious and stable promotion effect on promoting the formation of the bovine embryo blastula. The NAAG is high in compatibility with the existing in-vitro embryo culture system, low in application cost, and capable of being directly added into the existing conventional bovine in-vitro embryo culture system for use without changing basic formula of culture medium or cult