CN-122012407-A - Hybridoma cell strain secreting diallyl phthalate monoclonal antibody and application thereof

Abstract

The invention relates to a hybridoma cell strain secreting diallyl phthalate monoclonal antibody and application thereof, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain has excellent affinity and sensitivity to diallyl phthalate, the IC 50 of the diallyl phthalate reaches 500 ng/mL, and the monoclonal antibody has no cross reaction to structural analogues of phthalate, such as dimethyl phthalate, diethyl phthalate, dinonyl phthalate, butyl benzyl phthalate, diisobutyl phthalate, di (2-ethylhexyl) phthalate and diisononyl phthalate. Therefore, the monoclonal antibody can be used for preparing an immunodetection product of diallyl phthalate, and provides an efficient detection method and means for detecting the residues of diallyl phthalate in food.

Inventors

• QU AIHUA
• SONG SHANSHAN
• WU AIHONG
• GUO LINGLING
• XU XINXIN
• FENG QIANQIAN
• XU CHUANLAI
• KUANG HUA
• XU LIGUANG
• SUN MAOZHONG
• WU XIAOLING
• LIU LIQIANG
• HAO CHANGLONG

Assignees

• 江南大学

Dates

Publication Date

20260512

Application Date

20260130

Claims (10)

1. 1. A hybridoma cell strain is characterized in that the proposed taxonomy of the hybridoma cell strain is named as monoclonal antibody, and the preservation number is CGMCC NO.46746.
2. 2. The hybridoma cell line according to claim 1, wherein the hybridoma cell line is obtained by immunizing an animal with a complete antigen prepared from a hapten, wherein the hapten has the structural formula shown in formula I: 。
3. 3. the hybridoma cell strain according to claim 1, wherein said complete antigen is derived from said hapten-coupled carrier protein.
4. 4. The hybridoma cell line of claim 1, wherein said carrier protein comprises keyhole limpet hemocyanin.
5. 5. Use of the hybridoma cell line of any one of claims 1-4 for detecting diallyl phthalate.
6. 6. A monoclonal antibody secreted by the hybridoma cell line of any one of claims 1-4.
7. 7. Use of the monoclonal antibody of claim 6 for detecting diallyl phthalate.
8. 8. A diallyl phthalate detection product is characterized in that, the detection product comprises the monoclonal antibody of claim 6.
9. 9. The test product of claim 8, further comprising a coating antigen.
10. 10. The test product of claim 9, wherein the coating antigen is made from hapten-coupled carrier proteins, and wherein the carrier proteins comprise bovine serum albumin.

Description

Hybridoma cell strain secreting diallyl phthalate monoclonal antibody and application thereof Technical Field The invention relates to the technical field of immunodetection, in particular to a hybridoma cell strain secreting diallyl phthalate monoclonal antibody and application thereof. Background Diallyl phthalate (DAP) is an important organic compound, and is applied from the middle of the 20 th century, and along with the development of the petrochemical industry, the production technology of diallyl phthalate is continuously improved, so that diallyl phthalate is an important chemical raw material in the industries of plastics, rubber and the like. DAP is used as a cross-linking agent, can promote the formation of chemical bonds between polymer chains, form a more rigid three-dimensional structure, improve the heat resistance and chemical resistance of materials, and is commonly used in injection molding, extrusion molding and other processes. DAP has low toxicity, tear promoting effect, and can stimulate mucous membrane and skin to cause dermatitis. Meanwhile, the water-based composite material has extremely high toxicity to aquatic organisms, possibly has long-term adverse effect on the water environment, thus, the relevant safety regulations need to be followed in use and handling, avoiding release into the environment. Currently, the detection method of diallyl phthalate residues is mainly an instrument analysis method, including gas chromatography, gas chromatography-mass spectrometry, and the like. The methods have reliable results and high sensitivity, and related technical standards are available for reference. However, expensive instruments, special operators and complicated sample pretreatment, high cost and long time are required, so that the requirements of quick and simple field detection cannot be better met. Therefore, the establishment of a rapid and simple diallyl phthalate detection method is of great significance. The enzyme-linked immunosorbent assay (ELISA) is a very efficient, sensitive and rapid detection method, is suitable for on-site rapid detection of a large number of samples, and provides a novel detection way for diallyl phthalate. The enzyme-linked immunosorbent assay needs to provide a monoclonal antibody against diallyl phthalate, and the prior art lacks a monoclonal antibody against diallyl phthalate. Disclosure of Invention Therefore, the technical problem to be solved by the invention is to overcome the defect of lack of a monoclonal antibody aiming at diallyl phthalate in the prior art. In order to solve the technical problems, the invention provides a hybridoma cell strain secreting diallyl phthalate monoclonal antibody and application thereof. The hybridoma cell strain capable of secreting the diallyl phthalate monoclonal antibody is obtained through multiple screening, the diallyl phthalate monoclonal antibody secreted by the hybridoma cell strain has excellent affinity and sensitivity to diallyl phthalate, the IC 50 of the diallyl phthalate reaches 500 ng/mL, and the diallyl phthalate structural analogues such as dimethyl phthalate, diethyl phthalate, dinonyl phthalate, butyl benzyl phthalate, diisobutyl phthalate, di (2-ethylhexyl) phthalate and diisononyl phthalate have no cross reaction. Therefore, the monoclonal antibody can be used for preparing an immunodetection product of diallyl phthalate, and provides an efficient detection method and means for detecting the residues of diallyl phthalate in food. The first object of the present invention is to provide a hybridoma cell line, which is proposed to be named as monoclonal antibody in taxonomy and has the preservation number of CGMCC No.46746. Further, the hybridoma cell strain is obtained by immunizing an animal with a complete antigen prepared from a hapten, wherein the structural formula of the hapten is shown as formula I: 。 further, the complete antigen is derived from the hapten-coupled carrier protein. Further, the carrier protein comprises keyhole limpet hemocyanin. The second object of the invention is to provide an application of the hybridoma cell strain in detecting diallyl phthalate. The third object of the present invention is to provide a monoclonal antibody secreted by the hybridoma cell strain. The fourth object of the invention is to provide an application of the monoclonal antibody in detecting diallyl phthalate. A fifth object of the present invention is to provide a diallyl phthalate detection product comprising the monoclonal antibody as described above. Further, the detection product also comprises a coating source. Further, the coating antigen is prepared from hapten coupled carrier proteins, wherein the carrier proteins comprise bovine serum albumin. Compared with the prior art, the technical scheme of the invention has the following beneficial effects: The monoclonal antibody secreted by the hybridoma cell strain has good specificity and detection sensitivity (IC 50 of diallyl phtha