CN-122012410-A - Strong-schizolysis vibrio alginolyticus phage vB_ ValP _R38Z and application thereof

Abstract

The invention discloses a vibrio alginolyticus phage vB_ ValP _R38Z with strong hydrolytic property and application thereof. Vibrio alginolyticus phage (Vibrio alginolyticus phage) vB_ ValP _R38Z, accession number: GDMCC NO:67439-B1. The phage keeps higher activity under the conditions of 4-25℃, pH-8. The rapid adsorption and short latency characteristics of phage R38Z can obviously inhibit host growth at early infection, show high-efficiency cracking capability, and can rapidly complete adsorption, replication and cracking processes. Genomic analysis shows that R38Z has higher genetic safety, has no virulence, resistance and lysogenic related genes in the genome, carries multiple tRNA genes, and is beneficial to optimizing protein translation efficiency and infection adaptability. These characteristics together constitute an important advantage of R38Z in phage therapy applications.

Inventors

• Jiang Huaijin
• YANG YUNLAN
• ZHANG RUI
• LIU FEIFEI
• ZHANG QIFENG
• YANG HAN
• HUANG HAOYU

Assignees

• 深圳大学

Dates

Publication Date

20260512

Application Date

20251225

Claims (10)

1. 1. Vibrio alginolyticus phage (Vibrio alginolyticus phage) vB_ ValP _R38Z, accession number: GDMCC NO:67439-B1.
2. 2. A microbial preparation for controlling Vibrio alginolyticus or Vibrio Erwinia, characterized by comprising the phage vB_ ValP _R38Z as an active ingredient according to claim 1.
3. 3. Use of the phage vb_ ValP _r38z of claim 1 or the microbial preparation of claim 2 for the prevention and/or killing of vibrio alginolyticus or vibrio erwinis.
4. 4. Use of a bacteriophage vb_ ValP _r38z according to claim 1 or a microbial preparation according to claim 2 for the preparation of a product for the prevention and/or killing of vibrio alginolyticus or vibrio erwinis.
5. 5. The use according to claim 4, wherein said vibrio alginolyticus and said vibrio Erwinis from a body of water for penaeus vannamei cultivation.
6. 6. The use according to claim 4, wherein the Vibrio alginolyticus is Vibrio alginolyticus D69-TY10.
7. 7. The use according to claim 4, wherein the product comprises a pharmaceutical product, a disinfectant, a water scavenger, a feed or a feed additive.
8. 8. A product for controlling infection by vibrio alginolyticus or vibrio erwinis comprising the phage vb_ ValP _r38z of claim 1 or the microbial preparation of claim 2.
9. 9. The product of claim 9, wherein the vibrio alginolyticus is vibrio alginolyticus D69-TY10.
10. 10. A method for in vitro prevention and treatment of harmful infection of vibrio alginolyticus or Vibrio Erwinicus, characterized in that the microbial preparation of claim 1 and the phage vB_ ValP _R38Z or claim 2 are utilized to kill vibrio alginolyticus or Vibrio Erwinicus in vitro.

Description

Strong-schizolysis vibrio alginolyticus phage vB_ ValP _R38Z and application thereof Technical Field The invention belongs to the field of microorganisms, and particularly relates to a vibrio alginolyticus phage vB_ ValP _R38Z with strong degradability and application thereof. Background With the continuous expansion of the aquaculture scale and the improvement of the intensive level, the problems of water environment eutrophication, ecological imbalance, pathogenic microorganism transmission and the like are increasingly prominent. The frequency of bacterial diseases becomes a main bottleneck for restricting the sustainable development of industry, not only causes the increase of the death rate of the cultured animals and aggravation of economic loss, but also threatens the ecological safety of water areas. Vibrio (Vibrio) is an important pathogenic bacteria of various marine animals and is widely distributed in the salty water environments such as oceans, fresh water, estuaries and the like. The vibriosis caused by vibriosis has the characteristics of high transmission speed, strong pathogenicity, high death rate and the like, and can cause serious lesions and even large-scale death of aquatic animals such as fish, shrimp, shellfish and the like. Typical pathogenic processes include adhesion of the pathogen to the host surface and colonization, invasion of tissues, amplification and toxin production in vivo, ultimately leading to death of the host. Wherein, vibrio alginolyticus (Vibrio alginolyticus) is a common highly pathogenic strain in coastal aquaculture, has obvious regional epidemic characteristics, can cause obvious morbidity and mortality, and causes serious economic loss to the aquaculture industry. At present, antibiotics are still the most common measure for controlling vibriosis. However, the problem of antibiotic resistance and its risk of diffusion in aquatic environments has attracted considerable attention. Although non-antibiotic means such as vaccines, probiotics, immunostimulants and water body improvers can inhibit the occurrence of vibriosis to a certain extent, the prevention and control effects are often limited by long research and development period, slow action, strong environmental sensitivity and the like. In view of this, phage therapy (PHAGE THERAPY) is becoming a hotspot for aquatic disease control research as a highly specific, biodegradable and environmentally friendly natural antimicrobial strategy. Phage therapy is a two-step process involving penetration of phage into target bacteria and then killing of the bacteria. Phage (phase) is a bacterial virus, cell-free structure, consisting essentially of a protein capsid and genetic material nucleic acid contained therein. Phages can be classified into virulent phages, which invade bacterial cells, destroy the cell walls by enzymatic action, and lyse bacteria after biosynthesis and assembly using host resources. Phage therapy is an antibiotic alternative with great potential, but most phages have the problems of slow adsorption, long latency period and the like, and it is imperative to find vibrio alginolyticus phage with rapid lysis capability and gene safety. Disclosure of Invention The invention aims to provide vibrio alginolyticus phage vB_ ValP _R38Z and application thereof, and provides technical support for development of biological agriculture and related industries. A first object of the present invention is to provide a vibrio alginolyticus phage (Vibrio alginolyticus phage) vB-ValP-R38Z, accession number GDMCC NO:67439-B1. A second object of the present invention is to provide a microbial preparation for controlling Vibrio alginolyticus or Vibrio Erwini, which contains the above-mentioned phage vB_ ValP _R38Z as an active ingredient. A third object of the present invention is to provide the use of the phage vB_ ValP _R38Z described above or the microbial preparation described above for preventing and/or killing Vibrio alginolyticus or Vibrio Erwini. The fourth object of the present invention is to provide the use of the phage vB_ ValP _R38Z or the microbial preparation described above for the preparation of a product for preventing and/or killing Vibrio alginolyticus or Vibrio Erwini. Preferably, the vibrio alginolyticus and the vibrio Erwinis come from a penaeus vannamei boone culture water body. Preferably, the vibrio alginolyticus is vibrio alginolyticus D69-TY10. Preferably, the product comprises a pharmaceutical product, a disinfectant, a water purifying agent, a feed or a feed additive. A fifth object of the present invention is to provide a product for controlling infection by Vibrio alginolyticus or Vibrio Erwini, which contains the phage vB_ ValP _R38Z described above or the microbial preparation described above. Preferably, the vibrio alginolyticus is vibrio alginolyticus D69-TY10. The sixth object of the present invention is to provide a method for controlling harmful infection of vibrio alginolyticus o