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CN-122012415-A - Phage of schizolysis salmonella pullorum and application thereof

CN122012415ACN 122012415 ACN122012415 ACN 122012415ACN-122012415-A

Abstract

The invention discloses a phage of a lytic salmonella pullorum and application thereof, wherein the phage has a polyhedral head and a contractile tail, the average diameter of the head is about 71nm, the tail is about 126nm, the phage belongs to the myophagostimulaceae, the phage can lyse 15 salmonella pullorum in 18 salmonella clinical isolates, the incubation period is about 40 minutes, the lysis period is about 170 minutes, and the lysis amount is up to 142 PFU/cell. The phage of the salmonella pullorum has the advantages of wide host spectrum, high cleavage efficiency, low optimal infection complex number, low production cost and the like, and clinical experiments show that the phage can obviously reduce the mortality rate of infected chicks, reduce the detection rate of salmonella in viscera, alleviate pathological damage, purify chicken groups after long-term use, improve the laying rate and the egg quality, and provide a safe, efficient, economic and environment-friendly new prevention and control scheme of salmonella pullorum for poultry industry.

Inventors

  • WANG JINZI
  • ZHANG LIMIN
  • CHEN QIUYING
  • ZHANG HONGYUN
  • LI ZHEXIN
  • LIU ZIHAN
  • YANG FEI
  • LIAO TINGTING

Assignees

  • 广西民族大学
  • 广西悦牧生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260319

Claims (7)

  1. 1. A bacteriophage of salmonella pullorum having a polyhedral head and a contractile tail, the average head diameter being about 71nm and the tail being about 126nm, belonging to the family myophagiidae.
  2. 2. The phage of Salmonella pullorum as claimed in claim 1, which lyses 15 of the 18 clinical isolates of Salmonella pullorum.
  3. 3. The phage of Salmonella pullorum as claimed in claim 1, wherein the incubation period is about 40 minutes, the lysis period is about 170 minutes, and the lysis amount is up to 142 PFU/cell.
  4. 4. The phage of Salmonella pullorum having lytic activity of claim 1, wherein the optimal multiplicity of infection is 0.0001.
  5. 5. The phage of Salmonella pullorum as claimed in claim 1, wherein the good lytic activity is maintained in an environment of pH 4≤11.
  6. 6. The phage of Salmonella pullorum as claimed in claim 1, wherein the phage exhibits good activity at a temperature of 30-50 ℃.
  7. 7. The use of a bacteriophage of salmonella pullorum as set forth in any one of claims 1 to 6, wherein the product comprises a feed additive, a veterinary drug or a disinfectant.

Description

Phage of schizolysis salmonella pullorum and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to a phage of schizolysis salmonella pullorum and application thereof. Background Salmonella pullorum is a host-specific pathogen with serious harm in the poultry industry, and has wide transmission path and large prevention and control difficulty. The pathogen can cause pullorum disease, has extremely strong pathogenicity to chickens, causes high morbidity and high mortality of chickens, causes direct economic loss to a breeding ground, is in a recessive infection state after the chickens become adult chickens and bred chickens, becomes a long-term carrier, has serious influence on the reproductive performance of the chickens, expands epidemic situation range through vertical transmission (infection of offspring caused by invasion of reproductive system) and horizontal transmission (diffusion among chickens), forms continuous infection circulation, and seriously restricts the healthy development of poultry industry. At present, the prevention and control of salmonella pullorum in the poultry industry in China still mainly depend on antibacterial drugs. However, the current situation of long-term repeated use and unreasonable medication causes a series of serious problems that on one hand, bacterial drug resistance is increasingly aggravated, so that the control effect of the traditional antibacterial drugs is continuously reduced, partial bacterial strains are even developed into multi-drug resistant bacteria, so that clinical prevention and control are in a passive situation of invalid medication, on the other hand, the antibacterial drug residues enter a food chain through poultry products (meat and eggs), thus forming potential threat to human health, and meanwhile, the ecological balance is destroyed, and the industrial development trend of green cultivation is contrary. Therefore, developing a salmonella pullorum prevention, control and treatment technology which is safe, efficient, environment-friendly and capable of avoiding the drug resistance problem has become a key requirement to be solved in the current poultry industry. Phages are a class of viruses that specifically infect bacteria (including actinomycetes, fungi, and prokaryotes), and their uniqueness provides an ideal concept for solving the above problems. Phage widely exist in natural environments such as sewage, excrement and the like, have strict host specificity, attack only against one or more specific bacteria, have no infectivity to human beings, animals and plants, have extremely high safety, can not cause environmental pollutant residues, and are in accordance with the environment-friendly cultivation concept. The mechanism of action of the microbial inoculum is that after the microbial inoculum contacts with host bacteria, the microbial inoculum can be quickly adsorbed and invaded into the bacteria for proliferation, and finally, the progeny bacteriophage is released by cracking the host bacteria, and can further infect surrounding target bacteria to form an exponential proliferation effect, so that target pathogenic bacteria can be effectively killed in a short time. In addition, the drug resistance generation rate of the phage is far lower than that of the bacteria on antibiotics, the bacteriolysis effect is not limited by the existing drug resistance of the bacteria, meanwhile, the phage screening period is short, the preparation process is simple, the production cost is low, the commercial scale production is convenient to realize, and the phage has great potential of replacing the traditional antibacterial drug for controlling the salmonella pullorum. Disclosure of Invention The invention aims to provide a phage of schizolysis type salmonella pullorum, which aims to solve the problems of drug resistance, drug residue and the like existing in the prior art that antibacterial drugs are used for preventing and controlling salmonella pullorum in the background technology. In order to achieve the aim, the invention provides a bacteriophage of the salmonella pullorum with a splitting property, which has a polyhedral head and a contractile tail, wherein the average diameter of the head is about 71nm, and the tail is about 126nm, and belongs to the myophagiidae. Preferably, the said strain is capable of cleaving 15 strains of salmonella pullorum in 18 clinical isolates of salmonella. Preferably, the incubation period is about 40 minutes, the lysis period is about 170 minutes, and the lysis amount is up to 142 PFU/cell. Preferably, the optimal multiplicity of infection is 0.0001. Preferably, the high cleavage activity is maintained in an environment with pH value of 4-11. Preferably, the said compounds exhibit good activity at temperatures of 30-50 ℃. Preferably, the product comprises a feed additive, a veterinary or disinfectant. Compared with the prior art, the phage of the s