CN-122012418-A - Aeromonas dacarbazine bacteriophage vB_ AdhP _L12 and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and provides a dactylomonas volvatus bacteriophage vB_ AdhP _L12 and application thereof, wherein the dactylomonas volvatus bacteriophage vB_ AdhP _L12 is named as dactylomonas volvatus bacteriophage vB_ AdhP _L12 Aeromonas DHAKENSIS PHAGE vB_ AdhP _L12, has a preservation number of CCTCC NO: M2026249 and is preserved in China Center for Type Culture Collection (CCTCC) in the year 2026, 01 and 26. The invention enriches the treatment strategy for the dacarbazine and lays a foundation for inhibiting dacarbazine infection of crocodile breeding.

Inventors

• LI XUESONG
• YANG NUO
• ZHENG JIPING
• Zeng Yuerunan
• LIU SENWU
• ZHANG XIN
• PAN JINLONG
• DING HAIZHOU
• GUO GUIYING
• FAN LIXIA

Assignees

• 海南大学

Dates

Publication Date

20260512

Application Date

20260414

Claims (6)

1. 1. The dactylosin phage vB_ AdhP _L12 is characterized in that the dactylosin phage vB_ AdhP _L12 Aeromonas DHAKENSIS PHAGE vB_ AdhP _L12 is named as dactylosin phage taxonomic, and the dactylosin phage has a preservation number of CCTCC NO: M2026249 and has been preserved in China center for type culture collection (China for type culture Collection) at 26 of 01 of 2026.
2. 2. The dactylosin aeromonas phage vb_ AdhP _l12 according to claim 1, wherein the head of dactylosin aeromonas phage vb_ AdhP _l12 presents icosahedral stereo symmetry, the average head diameter is 52.03±2.50 nm, the phage contains a non-contractile tail, the average tail length is 13.39±2.05 nm, and the average diameter is 12.55±1.68 nm.
3. 3. The dactylosin phage vb_ AdhP _l12 according to claim 1, wherein the nucleic acid type of dactylosin phage vb_ AdhP _l12 is linear double-stranded DNA, full length 38749 bp, containing 42 open reading frames, free of antibiotic resistance genes, virulence factors and lysogenic related genes.
4. 4. A phage preparation comprising the dacarbazine aeromonas phage vb_ AdhP _l12 of any one of claims 1 to 3.
5. 5. The use of a phage preparation according to claim 4 for the prevention and treatment of dacarbazine infection.
6. 6. The use of a phage preparation according to claim 5 for the control of crocodile sepsis in culture.

Description

Aeromonas dacarbazine bacteriophage vB_ AdhP _L12 and application thereof Technical Field The invention belongs to the technical field of microorganisms, and relates to a dacarbazine A bacteriophage vB_ AdhP _L12 and application thereof. Background In recent years, the large-scale development process of crocodile culture industry in China is continuously accelerated, acute septicemia becomes an important epidemic disease which restricts the high-quality development of the industry, and the characteristics of urgent onset, quick transmission and high mortality rate of the acute septicemia cause great economic loss for the culture production, and also become a core key point of crocodile culture epidemic disease prevention and control work. Through separation and purification of early pathogens and molecular biological identification, the outbreak of the acute sepsis is proved to be triggered by bacterial infection, wherein dacarbazine aeromonas (Aeromonas dhakensis) is one of main pathogenic bacteria for inducing crocodile acute sepsis. At present, the prevention and control of bacterial septicemia of the cultured crocodile still takes an antibacterial drug as a core means, but the special antibacterial drug for aquaculture is limited by the agricultural rural department, and the antibacterial drug is not normally and reasonably used in production practice, so that pathogenic bacteria are easy to induce to generate drug resistance, the drug effect is greatly reduced, the drug dosage is continuously increased, a series of problems such as environmental pollution of the cultured water body, excessive drug residues of the cultured product and the like are caused, the development requirement of the green aquaculture is violated, and potential threats to the quality safety and ecological environment of the aquatic product are also formed. Therefore, developing an efficient, safe and environment-friendly green prevention and control technology to replace the traditional antibacterial drugs for preventing and controlling acute sepsis caused by aeromonas crocodile cultivation has become a key problem to be solved urgently in the current crocodile cultivation industry. Disclosure of Invention The invention aims to provide a dacarbazine bacillus bacteriophage vB_ AdhP _L12 and application thereof, enriches the treatment strategy for dacarbazine bacillus, and lays a foundation for inhibiting dacarbazine bacillus infection of crocodile culture. In order to achieve the technical purpose, the invention adopts the following technical scheme: The invention provides a strain of dactylomonas volvatus phage vB_ AdhP _L12, which is named as dactylomonas volvatus phage vB_ AdhP _L12 Aeromonas DHAKENSIS PHAGE vB_ AdhP _L12 in taxonomy, and has a preservation number of CCTCC NO: M2026249, and is preserved in China Center for Type Culture Collection (CCTCC) at 26 days of 2026. Further, the head of the dacarbazine-one phage vB_ AdhP _L12 presents icosahedral stereo symmetry, the average diameter of the head is 52.03+/-2.50: 2.50 nm, the phage contains a non-contractile tail, the average length of the tail is 13.39+/-2.05 nm, and the average diameter is 12.55+/-1.68: 1.68 nm. Further, the nucleic acid type of the dacarbazine phage vB_ AdhP _L12 is linear double-stranded DNA, the whole length 38749 bp contains 42 open reading frames, and no antibiotic resistance genes, virulence factors and lysogen related genes are provided. The invention also provides a phage preparation, which comprises the dacarbazine A phage vB_ AdhP _L12. The invention also provides application of the phage preparation, which is used for preventing and treating the infection of the aeromonas dacarbazine. Further, the phage preparation is used for preventing and treating crocodile septicemia in cultivation. The invention has the beneficial effects that: The invention utilizes the corresponding phages separated and enriched in the earlier stage, separates and identifies 1 new strong-lysis phage vB_ AdhP _L12 through a series of biological characteristic analysis and gene sequencing, and can specifically infect the aeromonas dactyli. Morphological results show that phage vB_ AdhP _L12 can form obvious plaques on a double-layer plate, a transmission electron microscope result shows that the phage is a short-tail phage, the optimal multiplicity of infection is 0.01, and one-step growth experiments show that the phage has extremely short incubation period which is less than 15min, has good stability under the conditions of temperature ranging from-20 ℃ to 50 ℃ and pH ranging from 4 to 11, and can resist chemical reagent treatment of 0.2% chloroform. The phage nucleic acid type is double-stranded DNA, the whole genome sequencing result shows that the phage has the whole length 38749 bp, contains 42 open reading frames, has no antibiotic resistance gene, virulence factor and lysogen related gene, and has high biological safety. The phylogenetic tree results showed that