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CN-122012433-A - Brown planthopper-sensitive related protein OsATL and coding gene and application thereof

CN122012433ACN 122012433 ACN122012433 ACN 122012433ACN-122012433-A

Abstract

The invention discloses a plant insect-resistant related protein, and a coding gene and application thereof. The protein provided by the invention is a protein (a) consisting of an amino acid sequence shown as a sequence 1 and a sequence shown in a sequence table or a protein (b) which is derived from the sequence 1 and the sequence 2 by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown as the sequence 1 and the sequence shown in the sequence table. The plant insect-resistance-related protein of the present invention affects insect resistance of plants. The knockout of the protein coding gene can improve the resistance of the plant brown planthopper, so that the plant insect-resistant transgenic plant can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

Inventors

  • WAN JIANMIN
  • LIU XI
  • TIAN YUNLU
  • LIU YUQIANG
  • WANG YUNLONG
  • HE JUN
  • ZHANG AORAN
  • ZHANG WENWEI
  • LI QI
  • Lu Fengdan
  • JIANG LING

Assignees

  • 南京农业大学

Dates

Publication Date
20260512
Application Date
20251229

Claims (7)

  1. 1. Protein OsATL, characterized in that said protein OsATL is selected from the following (a) or (b): (a) A protein consisting of the amino acid sequence shown in SEQ ID NO. 1; (b) And (b) a protein which is derived from the SEQ ID NO.1, is related to plant insect resistance and is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequences of the SEQ ID NO.1 and the amino acid sequences of the SEQ ID NO. 1.
  2. 2. A gene encoding the protein of claim 1.
  3. 3. The gene of claim 2, wherein the gene is a DNA molecule of any one of the following 1) to 4): 1) A DNA molecule shown in SEQ ID No. 2; 2) A DNA molecule shown in SEQ ID No. 3; 3) A DNA molecule which hybridizes under stringent conditions to the DNA sequence defined in 1) or 2) and which encodes the protein OsATL of claim 1; 4) A DNA molecule having more than 80% homology to the DNA sequence defined in 1) or 2) and encoding the protein OsATL of claim 1.
  4. 4. A knockout vector of claim 2 or 3.
  5. 5. The knockout vector of claim 4, wherein the knockout vector is a CRISPR-Cas9 vector, and the target sequence of the CRISPR-Cas9 vector is a DNA molecule represented by SEQ ID No.2 or SEQ ID No. 3.
  6. 6. Use of the protein OsATL of claim 1, or the gene of claim 2 or 3, or the knockout vector of claim 4 or 5 in plant breeding.
  7. 7. The use according to claim 9, wherein the rice planthopper resistance-enhanced rice can be obtained by down-regulating the expression level of the protein OsATL according to claim 1 in rice, or by knocking out the gene 2 or 3 according to claim 2 or 3 in rice, or by transferring the knock-out vector according to claim 4 or 5 into rice.

Description

Brown planthopper-sensitive related protein OsATL and coding gene and application thereof Technical Field The invention belongs to the field of genetic engineering, and particularly relates to a brown planthopper-like related protein OsATL of rice, and a coding gene and application thereof. Background Brown planthoppers are known as NILAPARVATA LUGENS (St å l), belong to the family of the plant hoppers of the order hemiptera, and are migratory and piercing-sucking pests which are specially harmful to rice. Adults and nymphs are clustered at the base of rice clumps, and the leaf sheath and stalk tissues of the rice are pierced by a piercing-sucking mouth organ to suck the juice of phloem. In addition, brown planthoppers are the main transmission media of the rice straw dwarf virus, the saw-tooth leaf dwarf virus and the like, no specific pesticide exists for the diseases, and the virus diseases are in an ascending trend in southeast Asia and other countries. And the large-scale brown planthoppers can also cause 'lice burning', which is the most typical and serious consequence of brown planthopper outbreaks. When the density of insect population is extremely high, a large amount of rice is eaten at the base of rice clumps in a short time, and the guide tissues are damaged, so that the rice plant base of whole clumps, whole sheets and even whole fields is blackened, rotted, lodged and withered before and after the period of milk ripeness, and the grains are not harvested. Chemical control remains an important way to control brown planthoppers when they burst in large numbers. However, the use of chemical pesticides in large quantities not only increases the burden of farmers and damages the ecological environment, but also enhances the drug resistance of brown planthoppers, which causes the rapid increase of the quantity and in turn aggravates the damage of brown planthoppers. Therefore, the breeding of brown planthopper resistant varieties and the excavation of resistance genes are called as more effective and environment-friendly ways. In recent years, along with the development of molecular markers and the construction of a high-density linkage map of rice, a plurality of main genes of rice brown planthopper resistance are respectively positioned on the 2 nd, 3 rd, 4 th, 6 th, 11 th and 12 th chromosomes of the rice, and a plurality of genes of rice brown planthopper resistance are cloned, but the molecular mechanism of the rice brown planthopper resistance is still to be further studied. Ubiquitination is a key protein post-translational modification in eukaryotes, and the ubiquitination is covalently connected with a target protein to regulate and control the stability, the positioning, the activity and the interaction of the target protein, and is involved in the core processes of cell homeostasis, signal transduction and the like. The ubiquitination process consists of three enzymes including ubiquitin activating enzyme E1, binding enzyme E2, and ligase E3. Among them, ubiquitin ligase E3 can specifically recognize substrates, so it is a key regulator of the ubiquitination pathway. Recently, more and more researches find that ubiquitin ligase plays an important role in plant immune regulation, on one hand, plants can target pathogenic bacteria through ubiquitination so as to degrade proteins, and can activate immune pathways and strengthen immunity. On the other hand, pathogens may also utilize the ubiquitination pathway to suppress the disease-resistant response of the host plant, thereby promoting self-infection. Although it has been found that ubiquitin ligases can regulate the immunity of plants against pathogenic bacteria, there are few reports on the involvement of ubiquitination in plant resistance, and there are fewer studies on whether ubiquitin ligases are involved in resistance of rice to brown planthoppers. Disclosure of Invention In order to solve the technical problems in the prior art, the application provides a brown planthopper-like related protein OsATL of rice, and a coding gene and application thereof. The technical scheme of the invention is as follows: it is a first object of the present invention to provide a protein OsATL, said protein OsATL being selected from the following (a) or (b): (a) A protein consisting of the amino acid sequence shown in SEQ ID NO. 1; (b) And (b) a protein which is derived from the SEQ ID NO.1, is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequences of the SEQ ID NO. and is related to plant insect resistance. It is a second object of the present invention to provide a gene encoding the aforementioned protein. Further, the gene is the DNA molecule according to any one of the following 1) to 4): 1) A DNA molecule shown in SEQ ID No. 2; 2) A DNA molecule shown in SEQ ID No. 3; 3) A DNA molecule which hybridizes under stringent conditions to the DNA sequence defined in 1) or 2) and which codes for