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CN-122012452-A - RSH1 coding gene related to rice grain number hybrid vigor establishment and application thereof

CN122012452ACN 122012452 ACN122012452 ACN 122012452ACN-122012452-A

Abstract

The invention relates to a RSH1 coding gene related to rice grain number hybrid vigor establishment and application thereof. Specifically, the invention discloses RSH1 protein and a biological material related to the same, wherein the protein is A1), A2) or A3), the amino acid sequence of A1) is the protein of SEQ ID No.3 in a sequence table, A2) is the protein with the same function obtained by substituting and/or deleting and/or adding one or more amino acid residues of the protein of A1), and A3) is fusion protein obtained by connecting protein labels at the N end or/and the C end of A1) or A2). The RSH1 protein and the related biological material thereof can be used for regulating and controlling the grain number and/or the yield of rice.

Inventors

  • ZHANG HONGLIANG
  • ZHANG QUAN
  • ZHU RUI
  • ZHANG ZHANYING
  • Cai Renfei
  • JIANG HONGYANG

Assignees

  • 中国农业大学三亚研究院
  • 海南华盈热作科技有限公司

Dates

Publication Date
20260512
Application Date
20260213

Claims (10)

  1. 1. A protein, characterized in that it is a protein of the following A1), A2) or A3): A1 A protein with an amino acid sequence shown as SEQ ID No. 2; a2 A protein having the same function as the protein represented by A1) obtained by substituting and/or deleting and/or adding one or more amino acid residues to the protein of A1); A3 Fusion proteins obtained by ligating protein tags at the N-terminal or/and C-terminal of A1) or A2).
  2. 2. The protein of claim 1, wherein said protein is derived from rice.
  3. 3. The protein-related biomaterial according to claim 1 or 2, characterized by being any one of the following B1) to B7): b1 A DNA molecule encoding the protein of claim 1; B2 An expression cassette comprising B1) said DNA molecule; b3 A recombinant vector comprising the DNA molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising B1) said DNA molecule, or a recombinant microorganism comprising B2) said expression cassette, or a recombinant microorganism comprising B3) said recombinant vector; B5 A transgenic plant cell line, transgenic plant tissue or transgenic plant organ comprising the DNA molecule of B1), or a transgenic plant cell line, transgenic plant tissue or transgenic plant organ comprising the expression cassette of B2); B6 A nucleic acid molecule which reduces the expression of the DNA molecule according to B1); B7 An expression cassette, a recombinant vector, a recombinant microorganism, a transgenic plant cell line, a transgenic plant tissue or a transgenic plant organ comprising the nucleic acid molecule of B6).
  4. 4. The biomaterial according to claim 3, wherein the DNA molecule of B1) is a gene as shown in B1) or B2) below: b1 A cDNA molecule with a coding sequence of a coding chain shown as SEQ ID No. 1; b2 A DNA molecule with a nucleotide sequence shown as SEQ ID No. 3.
  5. 5. The biomaterial of claim 3, wherein B6) the nucleic acid molecule is a gRNA targeting the gene encoding the protein of claim 1, the target sequence of the gRNA being the sequence shown in SEQ ID No. 4.
  6. 6. Use of the protein of claim 1 or 2, or the biomaterial of any one of claims 3-5, in any one of the following C1) -C3): c1 Regulating and controlling the grain number of the rice ears; c2 Regulating and controlling the rice yield; c3 Transgenic rice with hybrid vigor characteristics of spike number is cultivated.
  7. 7. The use according to claim 6, wherein the regulation of the number of rice ears is to increase the number of rice ears, and the regulation of the rice yield is to increase the rice yield by increasing the number of rice ears.
  8. 8. A method for increasing the grain number of ears and/or rice yield, which comprises inhibiting or reducing expression of a gene in a recipient rice to obtain a transgenic rice having grain number of ears and/or yield higher than the target rice and/or grain number heterosis characteristics of the recipient rice, wherein the gene is a gene encoding the protein of claim 1.
  9. 9. The method of claim 8, wherein the inhibiting or reducing expression of the gene in the recipient rice is accomplished by knocking out the gene in the recipient rice by a CRISPR/Cas9 system, the CRISPR/Cas9 system comprising expressing a plasmid comprising Cas9 and a gRNA having the target sequence of SEQ ID No.2 at positions 2141-2160.
  10. 10. A plant agent, characterized in that it comprises a protein according to claim 1 or 2 and/or a protein-related biological material according to any one of claims 3 to 5.

Description

RSH1 coding gene related to rice grain number hybrid vigor establishment and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to an RSH1 coding gene related to rice grain number hybrid vigor establishment and application thereof. Background Rice (Oryza sativa L.) has wide planting range, and more than half of people in China take rice as main food, and the rice yield plays a role in grain safety in China. 20. Three-line hybrid rice and two-line hybrid rice created by scientists in China have been widely popularized nationwide since the 70 th century, so that the rice unit yield is greatly improved. In the process, the improvement of the grain number of the rice spike and the effective utilization of the heterosis are key to the remarkable improvement of the yield of the rice. The number of rice ears is determined by each stage of branches and stems on a single rice ear and the seeds grown on the branches and stems. This also determines the complexity of the number of grains per spike, which is the largest among varieties among the three factors of yield and generally plays an important role in the genetic improvement of rice yield. The hybrid vigor refers to a natural phenomenon that the first filial generation obtained by crossing two parents with different genetic components is obviously superior to the parents in terms of growth vigor, yield and capability of propagating offspring, and is also the key of wide application of the hybrid rice at present. Although the improvement of grain number and effective utilization of heterosis make great contribution to the improvement of rice yield, the basic theoretical research on yield heterosis genes is still relatively lacking, and especially the research on obtaining new genes related to rice yield through heterosis positioning cloning is not yet reported, and the molecular mechanism of forming yield heterosis by a single rice gene is not yet clear. Therefore, the genetic foundation for building the rice grain number hybrid vigor is clarified, and the excavation of the rice grain number hybrid vigor gene is of great significance for the genetic improvement of rice varieties in the future. Disclosure of Invention Aiming at the defects and actual demands of the prior art, the invention provides an RSH1 coding gene related to rice grain number hybrid vigor establishment and application thereof. Specifically, the invention adopts the following technical scheme: In a first aspect, the present invention provides a protein which is a protein of the following A1), A2) or A3): A1 Protein RSH1 with the amino acid sequence shown as SEQ ID No. 2; a2 A protein having the same function as the protein represented by A1) obtained by substituting and/or deleting and/or adding one or more amino acid residues to the protein of A1); A3 Fusion proteins obtained by ligating protein tags at the N-terminal or/and C-terminal of A1) or A2). In one or more embodiments, the protein is derived from rice, preferably the rice variety is Nipponbare. In a second aspect, the present invention provides a biomaterial associated with a protein according to the present invention, which is any one of the following B1) to B7): B1 A DNA molecule encoding a protein RSH1 according to the invention; B2 An expression cassette comprising B1) said DNA molecule; b3 A recombinant vector comprising the DNA molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising B1) said DNA molecule, or a recombinant microorganism comprising B2) said expression cassette, or a recombinant microorganism comprising B3) said recombinant vector; B5 A transgenic plant cell line, transgenic plant tissue or transgenic plant organ comprising the DNA molecule of B1), or a transgenic plant cell line, transgenic plant tissue or transgenic plant organ comprising the expression cassette of B2); B6 A nucleic acid molecule which reduces the expression of the DNA molecule according to B1); B7 An expression cassette, a recombinant vector, a recombinant microorganism, a transgenic plant cell line, a transgenic plant tissue or a transgenic plant organ comprising the nucleic acid molecule of B6). In the above biological material, the expression cassette refers to a DNA capable of expressing a corresponding protein in a host cell, and the DNA may include not only a promoter for promoting transcription of a related gene but also a terminator for terminating transcription of a related gene. Further, the expression cassette may also include an enhancer sequence. Promoters useful in the present invention include, but are not limited to, constitutive promoters, tissue, organ and development specific promoters, and inducible promoters, and can be selected by one of skill in the art according to actual needs. The above biological materials are not limited to the recombinant vectors, and those skilled in the art can sele