Search

CN-122012514-A - Common thrips death related gene MuNICE and application thereof

CN122012514ACN 122012514 ACN122012514 ACN 122012514ACN-122012514-A

Abstract

The invention discloses a death-related gene MuNICE and application thereof of common thrips, wherein the nucleotide sequence of the death-related gene MuNICE is shown as SEQ ID NO. 1. The invention discovers that the dsRNA of MuNICE genes fed by common thrips can cause death of common thrips, has good lethal effect on common thrips with different developmental stages, and is obviously superior to the dsRNA of other insect lethal related genes. And further establishes a method for preventing and treating common thrips, which can effectively prevent and treat common thrips, and provides a new thought and a new way for effectively and environmentally-friendly preventing and treating common thrips.

Inventors

  • PAN HUIPENG
  • YU YUANBIN
  • YANG CHUNXIAO
  • ZHANG CHAOQUN
  • TANG RIYUAN
  • ZHONG ZEXIN
  • LI JIANXING
  • CHEN JIAN

Assignees

  • 华南农业大学

Dates

Publication Date
20260512
Application Date
20241108

Claims (10)

  1. 1. A gene MuNICE related to death of common thrips is characterized in that the nucleotide sequence of the gene is shown as SEQ ID NO. 1.
  2. 2. A dsRNA for controlling common thrips, which targets the death-related gene MuNICE according to claim 1.
  3. 3. The dsRNA of claim 2, wherein the nucleotide sequence of said dsRNA is set forth in SEQ ID No. 2.
  4. 4. The dsRNA of claim 3, further comprising a promoter sequence at the 5 'and/or 3' end of said dsRNA.
  5. 5. The dsRNA of claim 3, wherein the promoter sequence is a T7 promoter sequence, and the nucleotide sequence of the T7 promoter is shown in SEQ ID NO. 4.
  6. 6. A method for controlling common thrips, characterized in that common thrips is fed with the dsRNA according to any one of claims 2 to 5.
  7. 7. A kit for controlling common thrips comprising the dsRNA of any one of claims 2 to 5.
  8. 8. The kit of claim 7, further comprising an in vitro transcription reagent.
  9. 9. Use of the dsRNA of claim 2 for controlling common thrips.
  10. 10. Use of the dsRNA of claim 2 for the preparation of a product for controlling common thrips.

Description

Common thrips death related gene MuNICE and application thereof Technical Field The invention relates to the technical field of pest control, in particular to a common thrips death related gene MuNICE and application thereof. Background Common thrips Megalurothrips usitatus (Bagnall) belongs to the genus Thysanoptera, thrips and thrips. The pests cause serious harm to various crops, mainly including leguminous crops such as cowpea, kidney bean and hyacinth bean, and other crops such as tomatoes, loofah, corn and the like, and particularly in Hainan, guangdong and Guangxi areas, the occurrence and the harm are serious. Currently, field control of common thrips mainly depends on chemical control. However, common thrips have strong concealment, so that the dosage of the common thrips is increased and the common thrips are frequently applied in the prevention and treatment process, and the common thrips have different degrees of drug resistance to common drugs. In addition, the chemical control not only kills natural enemies, pollinating insects and other beneficial organisms, but also is difficult to degrade partial chemical synthetic insecticide, thereby causing serious threat to the ecological environment. By strengthening the farm control methods such as field management, cleaning the crop disease and residue, rotation and the like, the effect of preventing common thrips can only be achieved in the early stage of crop growth, and the farm control effect on the common thrips which has occurred in the late stage of crop growth is very limited. Therefore, a method for preventing and controlling common thrips with low cost, quick response and environmental friendliness is urgently needed at present. RNA interference (RNAINTERFERENCE, abbreviated RNAi) is a gene silencing technique triggered by endogenous or exogenous double-stranded RNA (dsRNA) that inhibits target gene expression by specifically degrading intracellular mRNA. The RNAi interference technology can control key genes of pest development or important behaviors, can indirectly block normal growth and reproduction of pests and even directly lead to death of the pests, thereby realizing the aim of pest control, being a novel green environment-friendly control method and having wide application prospect. Obtaining efficient and safe lethal target genes is a key to pest control by using RNAi technology. The NICE3 gene plays an important role in the epidermis formation process during the growth and development of insects. At present, the gene has relatively little research in insects, and has been widely studied in mammals. In humans, NICE3 is part of the Epidermal Differentiation Complex (EDC) which contains a large number of genes critical for human epidermal maturation, but the specific function of NICE3 in humans is still unclear and NICE3 may play an important role in the maintenance and differentiation of human hematopoietic stem cells. In blue pigs (LANTANG PIG), the expression of the NICE3 gene in multiple tissues was determined by qPCR, and the results showed that NICE3 gene was most expressed in the longus dorsi muscle, presumably related to the muscle development process. In the red pirate (Tribolium castaneum), the NICE5 gene in the NICE3 gene family was interfered with by the RNA injection method, and the result showed that the mortality rate 11 days after injection of red pirate pupae was 10% and the mortality rate 11 days after injection of larvae was 80%. This suggests that RNAi interferes with genes of the NICE gene family, potentially with some lethal effect on insects. Studies have shown that dsRNA has the highest RNAi interference efficiency against coleopteran and orthopteran insects, while lepidopteran insects have difficulty achieving RNAi, and more studies have shown that thysanopteran insects have the same lower RNAi efficiency. In the thrips palmi (THRIPS PALMI), the mortality rate of dsTpAPN and dsTpAPN to the thrips palmi is lower than 15%, and the mortality rate of dsTpAPN and dsTpAPN which are loaded with star-shaped cationic polymer (Star polycation, SPc) materials is only 23.3% and 30.0% compared with the control group dsGFP, so that the RNAi efficiency of thrips pests is low. At present, a high-efficiency thrips RNAi method is not researched and explored. Disclosure of Invention The invention aims to overcome the defects in the prior art and provides a common thrips death related gene MuNICE and application thereof. The first object of the invention is to provide a gene MuNICE related to death of common thrips. The second object of the invention is to provide dsRNA for preventing and treating common thrips. The third object of the invention is to provide a method for preventing and controlling common thrips. The fourth object of the invention is to provide a kit for preventing and treating common thrips. The fifth object of the invention is to provide the application of the dsRNA in preventing and controlling common thrips