CN-122012516-A - Sorghum aphid MsGld gene, coded protein, dsRNA and application thereof

CN122012516ACN 122012516 ACN122012516 ACN 122012516ACN-122012516-A

Abstract

The invention belongs to the technical field of biological control, and particularly relates to a sorghum aphid MsGld gene, a coding protein, dsRNA and application thereof. According to the invention, the sorghum aphid protein MsGld which can be secreted into sorghum cells is screened, and the protein and the coding gene thereof can inhibit the salicylic acid and active oxygen content in sorghum. According to the cDNA sequence of the sorghum aphid MsGld gene, the dsRNA designed for the gene is introduced into the sorghum aphid body in a manual feeding mode, and the dsRNA is found to be capable of remarkably inhibiting the sorghum aphid MsGld gene so as to influence the survival rate and the reproduction rate of the sorghum aphid, so that the MsGld gene can be used as an RNAi target gene for aphid control, thereby being beneficial to reducing pesticide use and maintaining ecological balance.

Inventors

LU YANHUI
SHANG XIAOYI
LIU XIAOWEI
LI QIANG
ZHOU YUJIE
LI JIANZHONG
PAN QIUBO
YANG YAJUN
LV ZHONGXIAN

Assignees

浙江省农业科学院

Dates

Publication Date: 20260512
Application Date: 20260413

Claims (7)

1. The application of the sorghum aphid gene MsGld, the sorghum aphid gene MsGld65 coding protein or the dsRNA for inhibiting the sorghum aphid gene MsGld65 in the preparation of the following products specifically comprises the following steps: (1) Preventing and controlling sorghum aphids or preparing products for preventing and controlling sorghum aphids; (2) Reducing the survival rate of the sorghum aphids or preparing a product for reducing the survival rate of the sorghum aphids; (3) Inhibiting the expression of the sorghum aphid gene MsGld or preparing a product for inhibiting the expression of the aphid sorghum aphid gene MsGld; (4) Regulating the content of the sorghum salicylic acid and the active oxygen or preparing a product for regulating the content of the sorghum salicylic acid and the active oxygen; (5) Inhibiting the propagation of sorghum aphids and/or the growth of sorghum aphids or preparing a product for inhibiting the propagation of sorghum aphids and/or the growth of sorghum aphids; the nucleotide sequence of the sorghum aphid gene MsGld is shown as SEQ ID NO.1, the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and the dsRNA is double-stranded RNA consisting of the nucleotide sequence shown as SEQ ID NO.3 and the reverse complementary sequence thereof.
2. The dsRNA for inhibiting expression of the sorghum aphid gene MsGld is characterized in that the nucleotide sequence of the sorghum aphid gene MsGld is shown as SEQ ID NO.1, and the dsRNA is double-stranded RNA consisting of the nucleotide sequence shown as SEQ ID NO.3 and the nucleotide sequence of the reverse complement thereof.
3. A biological material is characterized by comprising a sorghum aphid gene MsGld, a sorghum aphid gene MsGld, a protein encoded by the sorghum aphid gene 3965 or a dsRNA for inhibiting the sorghum aphid gene MsGld, wherein the nucleotide sequence of the sorghum aphid gene MsGld is shown as SEQ ID NO.1, the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and the dsRNA is double-stranded RNA consisting of nucleotides with the sequence shown as SEQ ID NO.3 and a reverse complementary sequence thereof.
4. The biomaterial of claim 3, wherein the biomaterial comprises recombinant DNA, an expression cassette, a plasmid vector, a viral vector, or an engineered bacterium.
5. A product containing an active ingredient, wherein the active ingredient comprises a sorghum aphid gene MsGld according to claim 1, a dsRNA encoding a protein of said sorghum aphid gene MsGld65 or inhibiting a sorghum aphid gene MsGld65 or a biological material according to any one of claims 3 to 4.
6. The product of claim 5, wherein the product has at least one of the following functions: (1) Preventing and controlling sorghum aphids; (2) Reducing the survival rate of the sorghum aphids; (3) Inhibiting expression of the sorghum aphid gene MsGld; (4) Regulating sorghum immune response and/or sorghum hydrogen peroxide content; (5) Inhibit the breeding of sorghum aphids and/or the growth of sorghum aphids.
7. Use of a product according to any one of claims 5 to 6 for biological control of sorghum aphids.

Description

Sorghum aphid MsGld gene, coded protein, dsRNA and application thereof Technical Field The invention belongs to the technical field of biological control, and particularly relates to a sorghum aphid MsGld gene, a coding protein, dsRNA and application thereof. Background Sorghum is the fifth largest food crop worldwide, and is one of the earliest cereal crops cultivated in China, and occupies an extremely important position in agricultural production. The sorghum has the stress resistance characteristics of stronger drought resistance, waterlogging resistance, salt and alkali resistance, barren resistance, high temperature resistance and the like, is edible, feed, brewing, biological energy, chemical materials and the like, and is the grain and feed dual-purpose crop and energy plant with the most development potential. However, the sorghum is damaged by sorghum aphids (MELANAPHIS SACCHARI) after emergence, and the number of single plant insects can reach 30000, so that the sorghum aphids are considered as main pests in the production of sorghum in China. The sorghum aphid invades the plant body by utilizing the piercing-sucking mouth organ to suck juice on the back surface of the sorghum leaf and the phloem of the stem, and discharges honeydew of high sugar, which not only causes mould growth, but also causes loss of nutrient substances, thereby causing crop yield reduction and even complete plant death. In addition, sorghum aphids are also a transmission medium of plant virus diseases such as cereal red leaf diseases, sugarcane yellow leaf diseases, sugarcane mosaic diseases and the like, and form indirect harm to crops. If insecticidal measures are taken, the yield loss caused by the sorghum aphid can reach 46% -78%, meanwhile, various economic properties of the sorghum are seriously affected, such as reducing the protein content of grains, obviously reducing the sugar content of stems, causing the problems of damaged stems, intolerance to storage and the like, causing destructive disasters, therefore, the effective control of the sorghum aphid is important for guaranteeing the yield and quality of the sorghum. The traditional method for preventing and controlling the sorghum aphid uses chemical pesticides, the chemical prevention and control effect is quick, the efficacy is high, the chemical pesticides are not limited by regions and time, but the large-scale use of the chemical pesticides can cause environmental pollution, the chemical pesticides do not accord with the green development subject, and the pesticide residues of the sorghum can cause harm to people and livestock, and the aphid can generate drug resistance after long-term use of the chemical pesticides, so that the prevention and control effect is lost. RNA interference (RNA INTERFERENCE, RNAI) refers to the efficient and specific degradation of double-stranded RNA (dsRNA) into cells that directs homologous mRNA, thereby inhibiting and down-regulating target gene expression. RNAi technology has been widely studied and applied in the field of modern agriculture, and silencing of target genes by artificial feeding, microinjection of dsRNA and nanoparticle-mediated methods has been widely used in the identification and analysis of insect gene function. Therefore, the excavation of the key genes with RNAi biological control in the sorghum aphids has important significance for guaranteeing the grain safety and the environmental safety of China. The information disclosed in this background section is only for enhancement of understanding of the background of the disclosure and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art that is well known to a person skilled in the art. Disclosure of Invention Aiming at the problems existing in the prior art, the invention aims to design and provide a technical scheme for the sorghum aphid MsGld gene, the coding protein, the dsRNA and the application thereof. The invention is realized by the following technical scheme: The first aspect of the invention provides a sorghum aphid gene MsGld and a nucleotide sequence of the sorghum aphid gene MsGld is shown as SEQ ID NO. 1. The second aspect of the invention provides the coding protein of the sorghum aphid gene MsGld, and the amino acid sequence of the coding protein is shown as SEQ ID NO. 2. In a third aspect, the invention provides a dsRNA for inhibiting expression of the sorghum aphid gene MsGld, wherein the dsRNA is double-stranded RNA consisting of nucleotides with a sequence shown in SEQ ID NO.3 and a reverse complement sequence thereof. In a fourth aspect, the present invention provides a biological material, wherein the sorghum aphid gene MsGld, the coding protein or the dsRNA is provided. Further, the biological material comprises recombinant DNA, an expression cassette, a plasmid vector, a viral vector or engineering bacteria. The fifth aspect of the invention provides an application of the so