Search

CN-122012517-A - Rice blast physiological race specific resistance gene Pi-hr, molecular marker and application

CN122012517ACN 122012517 ACN122012517 ACN 122012517ACN-122012517-A

Abstract

The invention provides a rice blast physiological race specific resistance gene Pi-hr, a molecular marker and application thereof, wherein the gene sequence of the rice blast physiological race specific resistance gene Pi-hr is shown as SEQ ID NO. 1. The molecular marker comprises 14-F and 14-R, the gene sequences of 14-F are shown in SEQ ID NO.4, and the gene sequence of 14-R is shown in SEQ ID NO. 5. The specific resistance gene Pi-hr is favorable for improving the pathogen infection capability of crop varieties, has important significance for realizing the polymerization cultivation of broad-spectrum resistant rice varieties by the multi-resistance gene, and can be used for improving the disease resistance capability of rice.

Inventors

  • LV QIMING
  • LOU JIANXING
  • SUN CHUANQING
  • LI YUANJIE

Assignees

  • 湖南杂交水稻研究中心

Dates

Publication Date
20260512
Application Date
20241111

Claims (7)

  1. 1. The physiological race specific resistance gene Pi-hr of the rice blast is characterized in that the gene sequence of the physiological race specific resistance gene Pi-hr of the rice blast is shown as SEQ ID NO. 1.
  2. 2. The rice blast physiological race-specific resistance gene Pi-hr according to claim 1, wherein the cDNA sequence of the rice blast physiological race-specific resistance gene Pi-hr is shown in SEQ ID NO. 2.
  3. 3. The rice blast physiological race-specific resistance gene Pi-hr according to claim 1, wherein the protein sequence of the rice blast physiological race-specific resistance gene Pi-hr is shown in SEQ ID NO. 3.
  4. 4. The rice blast physiological race-specific resistance gene Pi-hr according to claim 1, wherein the gene sequence of the 5' cap region of the rice blast physiological race-specific resistance gene Pi-hr is shown in SEQ ID No. 4.
  5. 5. The rice blast physiological race-specific resistance gene Pi-hr according to claim 1, wherein the gene sequence of the 3' polya region of the rice blast physiological race-specific resistance gene Pi-hr is shown in SEQ ID No. 5.
  6. 6. A molecular marker of physiological race-specific resistance gene Pi-hr against rice blast according to any one of claims 1 to 5, wherein said molecular marker comprises 14-F and 14-R, the gene sequence of said 14-F is shown in SEQ ID NO.6, and the gene sequence of said 14-R is shown in SEQ ID NO. 7.
  7. 7. Use of a physiological race-specific resistance gene Pi-hr of rice blast according to any one of claims 1 to 5 for improving disease resistance in rice.

Description

Rice blast physiological race specific resistance gene Pi-hr, molecular marker and application Technical Field The invention belongs to the technical field of biology, and particularly relates to a physiological race specific resistance gene Pi-hr of rice blast, a molecular marker and application thereof. Background Rice is taken as our ration to occupy the half-wall mountain of the grain crops in China. The rice blast is a rice fungal disease caused by ascomycetes, affects the whole growth and development period of rice, and can cause no harvest of rice particles when serious, thereby threatening 'our bowl'. Meanwhile, as the physiological race of the rice blast bacteria evolves faster, and most of the rice blast bacteria are prevented and controlled by adopting a chemical method (pesticide spraying) in production, in order to avoid land pollution and a large amount of pesticide residues, an effective biological prevention and control means is adopted, namely a biological method for cultivating a resistant variety to prevent and control rice blast in production is the most effective, most economical and safer, and the development of new rice blast resistance genes is the key for creating the resistant variety, and the utilization of molecular markers and rice colony positioning of the new rice blast resistance genes is an economic and effective means. In recent years, with the continuous breakthrough of molecular biotechnology means, systematic researches on rice blast resistance genes are further carried out, for example, the distribution of different resistance genes on different chromosomes is over 100, the rice blast main effect resistance genes located by using a molecular marker technology are distributed on all other chromosomes except for the third chromosome, more than half of the rice blast resistance genes are intensively distributed on the 6 th, 11 th and 12 th chromosomes of rice, the maximum number of the 11 th chromosome is approximately one fourth, the 12 th chromosome is approximately 15%, and the 6 th chromosome is approximately 14% close to the maximum number of the rice blast resistance genes. Furthermore, the main resistance genes of rice blast are distributed on the 12 th chromosome, a large resistance gene cluster exists in a region near the 12 th chromosome centromere, and although the rice blast resistance QTL locus found by rice exceeds one hundred, 38 genes such as Pi37、Pit、Pish、Pi35、Pi64、Pib、pi21、Pi63、Pi9、Pi2、Pi-zt、Pid2、Pid3/Pi25、Pid3-A4、Pi50、Pigm、Pid4、Pi36、Pi5、Pi56、Pi54of/Pi54、Pik-m、Pb1、Pik、Pia、Pik-p、Pi1、Pi54rh、PiCO39、Pik-h、Pike、Pita、ptr、bsr-k1、BGR8, pi-jx and the like are successfully cloned at present, and most of the resistance genes cloned at present encode a protein of NLR domain. However, due to the characteristic that physiological seeds of rice blast are easy to mutate, new markers, selection resistance genes, auxiliary gene positioning and cloning are required to be developed, and a molecular mechanism of limited broad-spectrum disease resistance of excellent characters in resistance germplasm resources is reasonably integrated, so that more resistance genes are excavated for improving the yield of rice, brand new reference is provided for rice blast resistance breeding, more theoretical references are provided, and more rice materials are provided for breeding. Disclosure of Invention The invention aims to solve the technical problems of overcoming the defects in the prior art and providing a physiological race specific resistance gene Pi-hr of rice blast, a molecular marker and application thereof. The invention utilizes the recombinant inbred line population constructed by two parents with obvious resistance difference phenotype to initially locate the rice blast resistance gene on the eighth chromosome, further screens the backcross population constructed by the recombinant inbred line individual plant and the susceptible parent to identify a new rice blast resistance gene Pi-hr and corresponding molecular markers to detect the resistance gene, and combines the resistance gene molecular markers to assist breeding to effectively cultivate excellent germplasm with high resistance polygene polymerization. In order to solve the technical problems, the invention provides a rice blast physiological race specific resistance gene Pi-hr, wherein the gene sequence of the rice blast physiological race specific resistance gene Pi-hr is shown as SEQ ID NO. 1. Furthermore, the cDNA sequence of the rice blast physiological race specific resistance gene Pi-hr is shown as SEQ ID NO. 2. Furthermore, the protein sequence of the rice blast physiological race specific resistance gene Pi-hr is shown as SEQ ID NO. 3. Furthermore, the gene sequence of the 5' cap region of the rice blast physiological race specific resistance gene Pi-hr is shown in SEQ ID NO. 4. Furthermore, the gene sequence of the 3' polyA region of the rice blast physiological race specific resistance gene Pi-hr is show