CN-122012520-A - Plant piercing-sucking pest-related gene MED25 and application thereof

Abstract

The present invention discloses a plant susceptible to piercing and sucking pests related gene MED25 and its application, which relates to the field of genetic engineering technology. The MED25 gene is derived from maize, and its nucleotide sequence is shown in SEQ ID NO.1. The amino acid sequence of its encoded protein is shown in SEQ ID NO.2. The present invention proves for the first time that the mutation of the MED25 gene in maize causes the loss of its function, which can improve the resistance of maize to piercing and sucking pests. The MED25 gene participates in the resistance to piercing and sucking pests by transcriptional regulation of the expression of terpene synthase genes (TPSs genes), providing important theoretical and technical support for the cultivation of maize varieties resistant to piercing and sucking pests, and has important production and application value.

Inventors

• LI PEIJIN
• LI TINGCHUN
• ZHAO YIBING
• WANG TENGYUE
• TAO ZHEN
• WANG CHUANHONG
• TANG ZHIWEI
• DONG JINGHUI
• LEI SISI
• CUI MINGLIANG

Assignees

• 安徽农业大学

Dates

Publication Date

20260512

Application Date

20260129

Claims (9)

1. 1. The plant piercing-sucking pest-related gene MED25 is characterized in that the MED25 gene is derived from corn, and the nucleotide sequence of the MED25 gene is shown as SEQ ID NO. 1.
2. 2. The MED25 gene encoded protein of claim 1, wherein the encoded protein has an amino acid sequence shown in SEQ ID No. 2.
3. 3. Use of the plant-piercing-sucking pest-related gene MED25 of claim 1 for regulating resistance of a plant to piercing-sucking pests.
4. 4. The use according to claim 3, wherein said MED25 gene mutation causes a loss of function of the gene, increases plant resistance to piercing-sucking pests, and said MED25 gene overexpression reduces plant resistance to piercing-sucking pests.
5. 5. The use according to claim 4, wherein the plant is arabidopsis thaliana, brassica napus, maize, rice or sorghum.
6. 6. The use according to claim 5, wherein the maize is maize inbred line B73, B104.
7. 7. The use according to claim 4, wherein the piercing-sucking pest is an aphid.
8. 8. Use of the MED25 gene of claim 1 or the encoded protein of claim 2 in the preparation of a medicament for controlling piercing-sucking pests.
9. 9. The use according to claim 8, wherein the piercing-sucking pest is an aphid.

Description

Plant piercing-sucking pest-related gene MED25 and application thereof Technical Field The invention relates to the technical field of genetic engineering, in particular to a plant piercing-sucking pest-related gene MED25 and application thereof. Background The piercing-sucking pests (aphids) are widely distributed in the global scope, seriously threaten the growth of various plants such as grain crops, oil crops and the like, have the characteristics of small size, high propagation speed, strong migration capability and the like, can rapidly propagate on plants in a short time, propagate various plant viruses to cause plant growth weakness while the plant photosynthesis is threatened by mass propagation of the aphids, and bring huge economic loss to global agricultural production. At present, chemical control is a main means for reducing aphid hazard, however, with the large-scale use of various pesticides on the market, the improvement of the drug resistance of aphids to the pesticides has become a great challenge in the field of pest management, and long-term application of pyrethroid, organophosphorus, neonicotinoid (such as imidacloprid) and other pesticides leads to the enhancement of detoxification enzyme activity and the mutation of reduced sensitivity in aphids, and the control method finally leads to the vicious circle of 'drug consumption increase and pest resistance enhancement', and damages human health and simultaneously destroys ecological balance. In view of the phenomena of explosive resistance surge and ecological unbalance of pests caused by chemical control, the method is to excavate the plant endogenous pest-resistant related genes, deeply analyze the molecular mechanism of crop aphid invasion, accurately guide the natural resistance genes into target crops by means of modern biotechnology, hopefully cultivate new varieties with stable yield and pest resistance, not only can alleviate pest harm from the source, but also provide a set of safe, efficient and sustainable green plant protection system for crop pest resistance from the perspective of plant endogenous resistance and sensitive genes. Disclosure of Invention In order to overcome the defects of the prior art, the invention aims to provide a piercing-sucking pest-related gene MED25 and application thereof. The invention realizes the above purpose through the following technical scheme: The invention provides a piercing-sucking pest-related gene MED25, wherein the MED25 gene is derived from corn, and the nucleotide sequence of the MED25 gene is shown as SEQ ID NO. 1. The invention also provides a coded protein of the MED25 gene, and the amino acid sequence of the coded protein is shown as SEQ ID NO. 2. The invention also provides application of the plant piercing-sucking pest-related gene MED25 in regulating and controlling the resistance of plants to piercing-sucking pests. As a further optimization scheme of the invention, the MED25 gene mutation causes the gene function to be deleted, the resistance of the plant to the piercing-sucking pests is improved, and the overexpression of the MED25 gene reduces the resistance of the plant to the piercing-sucking pests. As a further optimization of the invention, the plant is Arabidopsis thaliana, rape, maize, rice or sorghum. As a further optimization scheme of the invention, the corn is a corn inbred line B73 and B104. As a further optimization scheme of the invention, the piercing-sucking insect is aphid. The invention also provides an application of the MED25 gene or the coded protein in preparing medicaments for controlling piercing-sucking pests. As a further optimization scheme of the invention, the piercing-sucking insect is aphid. The invention has the following beneficial effects: The invention proves for the first time that the MED25 gene mutation of the corn leads the gene to have the function missing, can improve the resistance of the corn to the piercing-sucking pests, and the MED25 gene participates in the piercing-sucking pests through the expression of the transcription regulation terpene synthase gene (TPSs gene), thereby providing important theoretical technical support for cultivating the piercing-sucking pest resistant corn varieties and having important production and application values. Drawings FIG. 1 is a full-length amplification electrophoretogram of the MED25 gene of the present invention; FIG. 2 is an identification chart of homozygous mutant MED25 of MED25 gene of the present invention; FIG. 3 is a graph showing comparison of aphid weight changes 7 days after forced feeding of the homozygous mutant MED25 of the wild type maize B73, MED25 gene of the present invention; FIG. 4 is a graph showing comparison of aphid reproduction rate changes after forced feeding of the homozygous mutant MED25 of the wild type maize B73, MED25 genes of the present invention for various periods of time; FIG. 5 is a graph showing comparison of aphid weight changes 7 days after for