CN-122012522-A - Transcription factor ZmbHLH gene and its coding protein and application in plant flowering phase regulation

Abstract

The invention discloses a transcription factor ZmbHLH gene, a coding protein thereof and application thereof in plant flowering phase regulation, and relates to the technical field of plant genetic engineering, wherein the nucleotide sequence of the transcription factor ZmbHLH gene is shown as SEQ ID NO.1, the amino acid sequence of the coding protein is shown as SEQ ID NO.2, the invention provides a transcription factor ZmbHLH gene, CDS full length 864bp of the gene codes 287 amino acids, a transgenic over-expression ZmbHLH gene maize strain remarkably advances the maize flowering phase (male drawing, silking and powder scattering), a knock-out ZmbHLH gene mutant maize strain remarkably postpones the maize flowering phase, a certain technical basis is laid for plant flowering phase regulation, and a selection is provided for genetic improvement of new varieties of plants, and the invention has important application value.

Inventors

• WANG CHUANHONG
• CUI MINGLIANG
• WANG LIMING
• LI TINGCHUN
• FENG YANG
• HAN KAIKAI
• TAO ZHEN
• LI PEIJIN
• WANG TENGYUE
• DONG JINGHUI
• WANG HANLIN
• Dou Sifang
• FENG YING

Assignees

• 安徽农业大学

Dates

Publication Date

20260512

Application Date

20260210

Claims (7)

1. 1. A transcription factor ZmbHLH gene is characterized in that the nucleotide sequence of the transcription factor ZmbHLH gene is shown as SEQ ID NO. 1.
2. 2. The protein encoded by the gene ZmbHLH of claim 1, wherein the amino acid sequence of the protein encoded by the gene ZmbHLH is shown in SEQ ID NO. 2.
3. 3. Use of the transcription factor ZmbHLH gene according to claim 1 for flowering phase regulation in plants.
4. 4. The use according to claim 3, wherein said transcription factor ZmbHLH gene knockout inhibits maize emasculation, silking and dusting, and delays maize flowering phase, and said transcription factor ZmbHLH gene overexpression promotes maize emasculation, silking and dusting, and shortens maize flowering phase.
5. 5. The use according to claim 4, wherein the transcription factor ZmbHLH gene promotes male drawing, silking and powdering of corn and shortens the flowering phase of corn by positively controlling expression of the florigen synthesis gene ZCN.
6. 6. The use according to claim 5, wherein the maize is maize inbred B104.
7. 7. A method for obtaining new corn germplasm is characterized in that the transcription factor ZmbHLH gene as a target gene is knocked out in a corn genome, the obtained positive seedling is the new corn germplasm, the corn of the new corn germplasm is subjected to male drawing, silk laying and powder scattering, and later than wild corn, the corn flowering period is prolonged, and similarly, the transcription factor ZmbHLH180 gene as a target gene is introduced into the corn genome for over-expression, the obtained positive seedling is the new corn germplasm, and the corn of the new corn germplasm is subjected to male drawing, silk laying and powder scattering, and the corn flowering period is shortened.

Description

Transcription factor ZmbHLH gene and its coding protein and application in plant flowering phase regulation Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to a transcription factor ZmbHLH gene, a coding protein thereof and application thereof in plant flowering phase regulation. Background The flowering phase is taken as an important stage of growth and development, is one of factors for determining yield, can be accurately regulated and controlled by a plurality of genes in the flowering process of plants, encodes a florigen protein (ZCN 8 gene), is synthesized in leaves, is transported to flower organ tissues for a long distance, regulates and controls the flowering process of plants, plays a central role in the flowering phase transformation and adaptation dimension process, overexpresses ZCN genes in plants, and can effectively promote early flowering of the plants. Corn (Zea mays) is used as a hermaphrodite heteromorphic crop, the proper flowering phase determines the rotation time, the natural pollination efficiency and the maturity, the breeding of corn varieties tends to be in a shorter flowering phase, ZCN genes regulate and control the flowering time of the corn through integrating various signal paths such as hormone, photoperiod, temperature and the like, and therefore, the identification and control of the upstream transcription factor for ZCN8 gene expression has important reference value for cultivating proper flowering phase varieties. The bHLH transcription factor has an alkaline spiral-ring-spiral structure and an alkaline region, plays an important role in regulating flowering, growth and development of plants and responding to adversity approaches, previous researches show that the bHLH can realize the regulation and control of flowering phase through various complex pathways, in arabidopsis, iron deficiency induced AtbHLH38, atbHLH100 and AtbHLH101 can interact with CONSTANS (CO) to influence the transcription activity of CO so as to regulate the expression of FT, and similarly CRYPTOCHROME-INTERACTING BHLH1 (CIB 1) is directly combined with a promoter region of FT to regulate the photoperiod flowering, and similarly, rice homologous proteins CIB1-LIKE are combined with a EARLY HEADING DATE 1 promoter to regulate the flowering time, however, the regulation and control mechanism of the bHLH on the flowering phase in corn is freshly reported, and the regulation and control mechanism of bHILH in corn is deeply analyzed, so that not only the flowering phase regulation and control network is enriched, but also theoretical basis is provided for breeding work. Disclosure of Invention The invention aims to solve the problems and provide a transcription factor ZmbHLH gene, a coded protein thereof and application thereof in plant flowering phase regulation. The invention realizes the above purpose through the following technical scheme: In order to overcome the defects in the prior art, the invention aims to provide a transcription factor ZmbHLH gene, a coded protein thereof and application thereof in plant flowering phase regulation. The invention realizes the above purpose through the following technical scheme: the invention provides a transcription factor ZmbHLH gene, the nucleotide sequence of the transcription factor ZmbHLH gene is shown as SEQ ID NO. 1. The invention also provides a coded protein of the transcription factor ZmbHLH180,180 gene, and the amino acid sequence of the coded protein of the transcription factor ZmbHLH180,180 gene is shown as SEQ ID NO. 2. The invention also provides application of the transcription factor ZmbHLH gene in the flowering phase regulation of plants. As a further optimization scheme of the invention, the transcription factor ZmbHLH gene knockout inhibits the male drawing, the silking and the powder scattering of corn, delays the flowering period of corn, and the transcription factor ZmbHLH gene overexpression promotes the male drawing, the silking and the powder scattering of corn, so that the flowering period of corn is shortened. As a further optimization scheme of the invention, the transcription factor ZmbHLH gene promotes the male extraction, the silking and the powder scattering of corn by positively regulating the expression of the florigen synthesis gene ZCN, so that the flowering period of the corn is shortened. As a further optimization of the invention, the corn is a corn inbred line B104. The invention also provides a method for obtaining the novel corn germplasm, which takes the transcription factor ZmbHLH gene as a target gene to be introduced into a corn genome for over-expression, and the obtained positive seedling is the novel corn germplasm, and the corn of the novel corn germplasm is subjected to male-drawing, spinning and powder scattering earlier than wild corn, so that the flowering period of the corn is shortened. The invention has the beneficial effects that: 1)