CN-122012524-A - Peanut bacterial wilt resistant NBS-LRR coding gene AhRRS3 and application thereof

CN122012524ACN 122012524 ACN122012524 ACN 122012524ACN-122012524-A

Abstract

The invention provides a peanut bacterial wilt resistant NBS-LRR coding gene AhRRS and application thereof, the nucleotide sequence of the gene AhRRS is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The invention converts arabidopsis thaliana and observes the disease resistance function thereof by constructing AhRRS over-expression vector. Research results show that after bacterial wilt infection, the arabidopsis disease-resistant variety and the disease-sensitive strain of the over-expressed gene AhRRS show obvious disease resistance, and the disease-sensitive strain shows the death state of the whole plant, and the above results show that the over-expressed AhRRS gene can improve the bacterial wilt resistance of the plant. The invention provides high-quality gene resources and theoretical basis support for bacterial wilt resistance genetic engineering breeding of plants.

Inventors

ZHUANG YUHUI
ZHANG YONGLI
ZHUANG WEIJIAN
CHEN HUA
ZHANG CHONG
CAI TIECHENG
YANG QIANG
SUN WENXIAN

Assignees

福建农林大学

Dates

Publication Date: 20260512
Application Date: 20260212

Claims (6)

1. The application of peanut bacterial wilt resistance gene AhRRS in improving the bacterial wilt resistance of plants is characterized in that the nucleotide sequence of the gene AhRRS3 is shown as SEQ ID NO. 1.
2. The method according to claim 1, wherein the amino acid sequence of the protein encoded by the gene AhRRS is shown in SEQ ID NO. 2.
3. The method according to claim 1, wherein the plant is Arabidopsis thaliana.
4. The method of claim 1, wherein the over-expression AhRRS of the gene increases the resistance of the plant to bacterial wilt.
5. The application of the expression vector of the over-expression peanut bacterial wilt resistance gene AhRRS in improving the bacterial wilt resistance of plants is characterized in that the nucleotide sequence of the peanut bacterial wilt resistance gene AhRRS is shown as SEQ ID NO.1, and the plants are arabidopsis thaliana.
6. A method for improving bacterial wilt resistance of plants is characterized in that AhRRS gene over-expression vector is genetically transformed into a receptor plant, the receptor plant is arabidopsis thaliana, and the nucleotide sequence of AhRRS gene is shown as SEQ ID NO. 1.

Description

Peanut bacterial wilt resistant NBS-LRR coding gene AhRRS3 and application thereof Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to a peanut bacterial wilt-resistant NBS-LRR coding gene AhRRS and application thereof. Background Peanuts (Arachis hypogaea L.) are important oil-based commercial crops in the world, have rich nutritional values, and are an indispensable important source of edible vegetable oil. The development of the peanut industry has important significance for guaranteeing the safety of the food of the folk life. Peanut bacterial wilt is a serious disease problem of peanuts, is caused by soil-borne bacterial wilt (Ralstonia solanacearum), causes wilting death of peanut plants through invasion of peanut roots, and severely restricts the yield of the peanuts. The host of ralstonia solanacearum also infects other important crops such as tobacco, potato, capsicum, eggplant and other commercial crops. However, no effective medicament can radically cure the bacterial wilt disease at present, so that high-quality resistance gene resources are obtained by carrying out positioning and function identification of peanut bacterial wilt disease resistance genes, and further, the cultivation of resistant varieties is promoted, thereby providing important application value for guaranteeing global grain and oil resources. Disclosure of Invention The invention aims to provide a peanut bacterial wilt resistant NBS-LRR coding gene AhRRS and application thereof. In order to achieve the above purpose, the present invention adopts the following technical scheme: In a first aspect, the invention provides application of peanut bacterial wilt resistance gene AhRRS to improvement of plant bacterial wilt resistance, and a nucleotide sequence of gene AhRRS is shown as SEQ ID NO. 1. Further, the amino acid sequence of the protein encoded by the gene AhRRS is shown as SEQ ID NO. 2. Further, the plant is peanut or arabidopsis thaliana. Further, over-expression AhRRS of the gene increases the resistance of the plant to bacterial wilt. In a second aspect, the invention provides application of an expression vector for over-expressing peanut bacterial wilt resistance gene AhRRS to improvement of bacterial wilt resistance of plants, wherein a nucleotide sequence of peanut bacterial wilt resistance gene AhRRS is shown as SEQ ID No.1, and the plants are peanuts or arabidopsis thaliana. In a third aspect, the invention provides a method for improving bacterial wilt resistance of plants, which is characterized in that AhRRS gene over-expression vectors are used for genetic transformation of receptor plants, the receptor plants comprise peanut and arabidopsis thaliana, and the nucleotide sequence of the AhRRS gene is shown as SEQ ID NO. 1. The invention has the beneficial effects that: The invention obtains a high-generation stable recombinant inbred line group (RIL group) through hybridization breeding of a peanut disease-resistant variety Yue oil 92 (YY 92) and a disease-resistant variety Xinhui granule (XHXL). Through multi-point planting of RIL population for many years, bacterial wilt is inoculated for resistance identification, the genotype of each RIL line is sequenced and identified, molecular markers are developed, and a high-density genetic map is constructed. Comprehensive genetic map and resistance phenotype identification, and fine positioning by using QTL to obtain candidate NBS-LRR coding gene AhRRS for resisting bacterial wilt. The bacterial wilt resistance gene AhRRS provided by the invention can be over-expressed in plants to improve the bacterial wilt resistance, and can improve the disease resistance of the plants against the bacterial wilt under bacterial wilt infection, thereby ensuring the normal growth and development of the plants. AhRRS3 the acquisition of the disease-resistant gene provides high-quality resistance gene resources for the genetic improvement of peanut and other important crops with important economic crops in bacterial wilt resistance. Drawings FIG. 1 shows the results of fine localization of the anti-bacterial wilt QTL of a Recombinant Inbred Line (RIL) population of peanuts, with the anti-bacterial wilt candidate gene being localized on chromosome 12, the Chr 12. FIG. 2 shows subcellular localization of peanut bacterial wilt resistance gene AhRRS for transient expression of Nicotiana benthamiana. FIG. 3 shows the disease resistance results of peanut bacterial wilt resistance gene AhRRS over-expression genetic transformation Arabidopsis thaliana to bacterial wilt, the phenotype of each plant without bacterial wilt inoculation, the phenotype of each plant after bacterial wilt inoculation, and the disease index of each plant. In the calculation formula of the disease index (D) = Σ (MixSi) ×100/(N×4), D represents the disease index, i represents the disease number, mi represents the number of plants with disease being i, si