Search

CN-122012526-A - Novel tea tree gene CsCRAP and cold-resistant application thereof

CN122012526ACN 122012526 ACN122012526 ACN 122012526ACN-122012526-A

Abstract

The invention belongs to the technical field of genetic engineering, and discloses a tea CsCRAP gene and cold-resistant application thereof. The CsCRAP gene is a novel tea tree cold-resistant gene, the nucleotide sequence is shown as SEQ ID NO.1, the amino acid sequence of the coded protein is shown as SEQ ID NO.2, and the protein is positioned on cell membranes. The gene CsCRAP can be obviously induced to be up-regulated by low-temperature stress, the leaf low-temperature damage can be obviously relieved by over-expression of the gene in the tea tree body, the low-temperature tolerance is improved, and the low-temperature damage is obviously aggravated by inhibiting the expression of the gene. The invention also provides a recombinant plasmid containing the gene and a transgenic engineering bacterium. The invention verifies CsCRAP that the gene positively regulates the cold resistance of tea trees for the first time, provides core gene resources for research on cold resistance mechanisms and cold resistance breeding of tea trees, can be used for genetic improvement of tea trees, improves the low-temperature tolerance of tea trees, and promotes sustainable development of tea industry.

Inventors

  • Xia Enhua
  • Ying Dandan
  • Zha Yinong
  • WANG YANLI
  • Yi Lianghui
  • TONG WEI

Assignees

  • 安徽农业大学

Dates

Publication Date
20260512
Application Date
20260323

Claims (7)

  1. 1. The tea tree CsCRAP gene is characterized in that the nucleotide sequence of the tea tree CsCRAP gene is shown in a sequence table SEQ ID NO. 1.
  2. 2. A tea tree CsCRAP protein is characterized in that the amino acid sequence of the tea tree CsCRAP protein is shown in a sequence table SEQ ID NO.2, and the tea tree CsCRAP protein is encoded by the tea tree CsCRAP gene of claim 1.
  3. 3. A recombinant plasmid comprising the tea CsCRAP gene of claim 1.
  4. 4. A transgenic engineering bacterium is characterized in that, the transgenic engineering bacteria comprise the recombinant plasmid of claim 4.
  5. 5. Use of the tea plant CsCRAP gene according to claim 1 for regulating plant cold tolerance, wherein said tea plant CsCRAP gene is overexpressed in plants to increase plant cold tolerance.
  6. 6. Use of a tea tree CsCRAP protein as claimed in claim 2 for modulating cold tolerance in plants, wherein said tea tree CsCRAP protein is used to enhance tolerance of plants to low temperature stress.
  7. 7. A method of improving cold tolerance of tea plants, wherein the gene of tea plant CsCRAP of claim 1 is overexpressed in the plant to alleviate cold damage to leaves of tea plants.

Description

Novel tea tree gene CsCRAP and cold-resistant application thereof Technical Field The invention relates to the technical field of genetic engineering, in particular to a tea CsCRAP gene and application thereof in regulating and controlling cold resistance of tea trees. Background Tea tree (CAMELLIA SINENSIS (L.) O.Kuntze) is a plant of the genus Camellia of the family Theaceae, and tea made from its buds and leaves is one of three non-alcoholic beverages in the world, contains multiple beneficial components and has health promotion effect, and the development relationship of tea industry is civil and national economy. In recent years, tea trees in winter and early spring frequently suffer from low-temperature injuries such as frost, cold in the spring and the like, so that the yield and quality of tea leaves are greatly reduced, and the tea trees die in severe cases. Tea trees like Wen Wei cold, low temperature becomes one of the most important and serious natural disasters in tea production, and the health sustainable development of tea industry is threatened. In order to cope with cold stress, tea trees evolve a series of molecular mechanisms to relieve the cell metabolic disorder caused by cold stress, and the molecular mechanisms mainly comprise a calcium ion signal path, an ICE-CBF-COR signal path, a carbohydrate metabolic path, an antioxidant path, a transcription factor regulation path and the like. At present, the cold-resistant research of tea trees focuses on single or few functional analyses of genes, the research of a systematic network regulation mechanism is less, and the development of key cold-resistant new genes and functional research thereof are urgently needed to be enhanced. The tea CsCRAP gene is a novel cold-resistant gene of the tea tree, which is excavated by analyzing large-scale gene co-expression network by utilizing transcriptome big data, the expression of the novel cold-resistant gene is obviously induced to be up-regulated by low temperature, the biological function of the tea tree CsCRAP gene in regulating and controlling the cold resistance of the tea tree is explored, the cold-resistant complex molecular mechanism of the tea tree is facilitated to be clarified, a new idea is provided for research on the cold-resistant mechanism of the tea tree, and an important theoretical basis is provided for breeding of the cold-resistant character of the tea tree. The invention aims to provide a tea CsCRAP gene and application thereof in regulating and controlling cold resistance of tea trees, solves the technical problems that in the prior art, the research of cold-resistant key novel genes of tea trees is insufficient, the research of cold-resistant molecular mechanisms is not systematic, and the research of cold-resistant genetic improvement is lack of core gene resources, provides a novel thought for the research of cold-resistant mechanisms of tea trees, and provides theoretical and practical reference bases for realizing the breeding of the cold resistance characters of tea trees. Disclosure of Invention The invention aims to provide a tea CsCRAP gene and application thereof in regulating and controlling cold resistance of tea trees, so as to solve the problems. In order to achieve the above object, the present invention provides the following technical solutions: the tea tree CsCRAP gene is characterized in that the nucleotide sequence of the tea tree CsCRAP gene is shown in a sequence table SEQ ID NO. 1. The amino acid sequence of the tea tree CsCRAP protein is shown in a sequence table SEQ ID NO.2, and the tea tree CsCRAP protein is encoded by the tea tree CsCRAP gene. A recombinant plasmid comprising the tea CsCRAP gene of claim 1. A transgenic engineering bacterium, which comprises the recombinant plasmid. An application of tea tree CsCRAP gene in regulating and controlling plant cold tolerance, wherein the tea tree CsCRAP gene is over-expressed in a plant body so as to improve the low temperature tolerance of the plant. Use of tea CsCRAP protein for regulating plant cold tolerance, said tea CsCRAP protein for enhancing plant tolerance to low temperature stress. A method of improving cold tolerance of tea plants, wherein the gene of tea plant CsCRAP according to claim 1 is overexpressed in the tea plant to alleviate cold damage to the leaves of tea plants. The invention has the beneficial effects that: The invention clones and verifies the tea CsCRAP gene and tea CsCRAP protein for regulating and controlling tea response to low temperature stress for the first time, and the two exert regulating and controlling effects in the tea response to low temperature stress process to determine the cold resistance formation of tea. The recombinant plasmid and the transgenic engineering bacteria containing the tea CsCRAP genes are provided, the research resources of the cold-resistant genes of the tea trees are enriched, a new thought is provided for the research of the cold-resistant m