CN-122012528-A - Azalea MYB transcription factor RhMYB and application thereof

Abstract

The invention relates to the technical field of plant genetic engineering, and discloses azalea MYB transcription factor RhMYB and application thereof. The nucleotide sequence of the azalea MYB transcription factor RhMYB is shown as SEQ ID NO.1, and the encoded amino acid sequence is shown as SEQ ID NO. 2. The MYB transcription factor RhMYB of the azalea is overexpressed in the azalea, so that the cold resistance of the azalea is improved. The rhododendron MYB transcription factor RhMYB and the application thereof provide a novel functional gene and a core target point for cold-resistant molecular breeding of horticultural plants, lay a theoretical and technical foundation for improving low-temperature stress resistance of plants and cultivating novel cold-resistant plant varieties by utilizing molecular means, and have important application values for improving quality and efficiency of the rhododendron industry and breeding cold-resistant varieties.

Inventors

• ZHOU HONG
• ZHANG HAO
• WANG XIUYUN
• YAO YAO
• XIA YIPING

Assignees

• 浙江大学

Dates

Publication Date

20260512

Application Date

20260401

Claims (10)

1. 1. The azalea MYB transcription factor RhMYB is characterized in that the nucleotide sequence of RhMYB is shown as SEQ ID NO. 1.
2. 2. The application of azalea MYB transcription factor RhMYB in improving azalea cold resistance is characterized in that azalea MYB transcription factor RhMYB is overexpressed in azalea, the cold resistance of azalea is improved, and the nucleotide sequence of azalea MYB transcription factor RhMYB is shown as SEQ ID NO. 1.
3. 3. The azalea RhMYB protein is characterized in that the amino acid sequence of the azalea RhMYB protein is shown as SEQ ID NO.2, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 1.
4. 4. The application of azalea RhMYB protein in improving the cold resistance of azalea is characterized in that the amino acid sequence of azalea RhMYB protein is shown as SEQ ID NO.2, the nucleotide sequence of the coding gene of the azalea RhMYB protein is shown as SEQ ID NO.1, and the coding gene of azalea RhMYB protein is overexpressed in azalea, so that the cold resistance of azalea is improved.
5. 5. A recombinant expression vector containing the azalea MYB transcription factor RhMYB as described in claim 1, wherein the recombinant expression vector is a plant over-expression vector and is pHB-YFP-RhMYB.
6. 6. A transgenic cell line comprising azalea MYB transcription factor RhMYB of claim 1.
7. 7. A transgenic recombinant bacterium comprising the azalea MYB transcription factor RhMYB of claim 1.
8. 8. Use of the recombinant expression vector of claim 5 or the transgenic cell line of claim 6 or the transgenic recombinant bacterium of claim 7 for improving cold resistance of azalea.
9. 9. A method for cultivating cold-resistant azalea is characterized in that azalea MYB transcription factor RhMYB with a nucleotide sequence shown as SEQ ID NO.1 is overexpressed in azalea plants, and cold-resistant azalea is obtained through screening and cultivation.
10. 10. The method according to claim 9, comprising the steps of transforming a recombinant expression vector comprising the azalea MYB transcription factor RhMYB having the nucleotide sequence shown in SEQ ID NO.1 into azalea plant cells, tissues or organs by means of Agrobacterium-mediated, gene gun, vacuum infiltration or microinjection, cultivating the transformed plant material into whole plants, and screening to obtain azalea plants with enhanced cold resistance.

Description

Azalea MYB transcription factor RhMYB and application thereof Technical Field The invention relates to the technical field of plant genetic engineering, in particular to azalea MYB transcription factor RhMYB and application thereof. Background Low temperature stress is one of the important environmental factors limiting the growth and geographical distribution of landscape plants. Rhododendron spp is an important ornamental plant and has wide application in landscaping, but partial evergreen azalea varieties are sensitive to low temperature, and the low temperature in winter easily causes leaf freezing, cell membrane damage and plant growth inhibition, so that ornamental value and popularization and application are affected. Plants under low temperature stress will initiate complex molecular regulatory networks to adapt to the stress environment, where transcription factors play a key role in regulating stress response. MYB transcription factor is one of the largest transcription factor families in plants, and has important functions in the processes of plant growth and development, secondary metabolism regulation, abiotic stress response and the like. However, at present, no functional report of azalea MYB genes in plant cold resistance regulation exists, and no related application of related azalea genes in cultivation of cold-resistant plant varieties is disclosed. Therefore, the azalea cold-resistant related MYB genes are identified by excavation, and important candidate genes can be provided for regulating and controlling the cold resistance of azalea and other important crops by utilizing a molecular means. Disclosure of Invention The invention aims to provide the azalea MYB transcription factor RhMYB and application thereof, wherein the azalea MYB transcription factor RhMYB is overexpressed in azalea, so that the cold resistance of the azalea can be improved, a novel functional gene and a core target point are provided for the cold-resistant molecular breeding of horticultural plants, theoretical and technical bases are laid for improving the low-temperature stress resistance of plants and cultivating novel cold-resistant plant varieties by utilizing molecular means, and the azalea MYB transcription factor RhMYB has important application values for improving quality and enhancing efficiency of azalea industry and breeding cold-resistant varieties. To achieve the above object, in a first aspect, the present invention provides a nucleotide sequence of rhododendron MYB transcription factor RhMYB, rhMYB8 as shown in SEQ ID No. 1. In a second aspect, the invention provides an application of azalea MYB transcription factor RhMYB in improving cold resistance of azalea, wherein azalea MYB transcription factor RhMYB is overexpressed in azalea, cold resistance of azalea is improved, and a nucleotide sequence of azalea MYB transcription factor RhMYB is shown as SEQ ID NO. 1. In a third aspect, the invention provides azalea RhMYB protein, the amino acid sequence of azalea RhMYB protein is shown as SEQ ID NO.2, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 1. In a fourth aspect, the invention provides application of azalea RhMYB protein in improving the cold resistance of azalea, wherein the amino acid sequence of azalea RhMYB protein is shown as SEQ ID NO.2, the nucleotide sequence of the coding gene is shown as SEQ ID NO.1, and the coding gene of azalea RhMYB protein is overexpressed in azalea, so that the cold resistance of azalea is improved. In a fifth aspect, the invention provides a recombinant expression vector comprising the azalea MYB transcription factor RhMYB as described above, wherein the recombinant expression vector is a plant over-expression vector, and is pHB-YFP-RhMYB. Further, the invention provides a transgenic cell line comprising azalea MYB transcription factor RhMYB as described above. Further, the invention provides a transgenic recombinant bacterium containing the azalea MYB transcription factor RhMYB. In a sixth aspect, the invention provides an application of the recombinant expression vector or the transgenic cell line or the transgenic recombinant bacterium in improving cold resistance of azalea. In a seventh aspect, the invention provides a method for cultivating cold-resistant azalea, wherein the MYB transcription factor RhMYB of the azalea with a nucleotide sequence shown as SEQ ID No.1 is overexpressed in azalea plants, and the cold-resistant azalea is obtained by screening and cultivating. Further, the method for cultivating cold-resistant azalea comprises the following steps of transforming a recombinant expression vector containing azalea MYB transcription factor RhMYB with a nucleotide sequence shown as SEQ ID NO.1 into azalea plant cells, tissues or organs in a mode of agrobacterium mediation, gene gun, vacuum permeation or microinjection, cultivating the transformed plant material into a complete plant, and screening to obtain the azalea plant with