CN-122012530-A - Application of SlIDD gene in improving tomato yield and sugar content in high-temperature environment

Abstract

The invention discloses an application of SlIDD gene in improving tomato yield and sugar content in a high-temperature environment, belonging to the technical field of genetic engineering. According to the invention, the endogenous gene SlIDD of the tomato is knocked out in the tomato by using a CRISPR/Cas9 gene editing technology, and phenotypic identification analysis shows that the fruit setting rate of the strain with the knocked-out SlIDD gene is obviously improved under a high-temperature condition, the single plant yield is obviously improved, and the contents of sugar substances such as sucrose, fructose, glucose and the like of tomato fruits in the knocked-out strain are obviously increased, so that the fruit quality is improved, and important gene resources and strategies are provided for synergistically improving the yield and the quality of the tomato fruits under a high-temperature stress condition.

Inventors

• HUANG BAOWEN
• LIN YUXIANG
• LI ZHENGGUO
• SHI YUAN
• DENG WEI
• FU JIA
• ZHU WEI
• DONG PAN

Assignees

• 重庆大学

Dates

Publication Date

20260512

Application Date

20260410

Claims (2)

1. 1. The application of SlIDD gene in improving tomato yield and sugar content in high-temperature environment is characterized in that the tolerance of tomato plants to high temperature is improved by inhibiting SlIDD gene expression or knocking out SlIDD gene, simultaneously the fruit setting rate and single plant yield of tomatoes are improved, the sucrose, fructose and glucose content of tomato fruits is improved, and the nucleotide sequence of SlIDD gene is shown as SEQ ID NO. 1.
2. 2. Use according to claim 1, characterized in that the high temperature is 30-35 ℃.

Description

Application of SlIDD gene in improving tomato yield and sugar content in high-temperature environment Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to an application of SlIDD gene in improving tomato yield and sugar content in a high-temperature environment. Background Tomatoes are important vegetable crops in the world, and high temperature stress has become a key bottleneck limiting the yield and quality of tomatoes. Therefore, the cultivation of 'favourable circumstance high-yield, stable-yield in adversity' environment intelligent tomato strains and the excavation of high-temperature-resistant main functional genes thereof become the core topic and the front hot spot of the current vegetable genetic breeding. The IDD (INDETERMINATE DOMAIN) family gene belongs to a subfamily of C2H2 type zinc finger family, is a highly conserved transcription factor in plants, widely participates in growth and development and stress response in plant species, and is a potential target gene for crop variety improvement. At present, research on IDD family genes mainly focuses on Arabidopsis thaliana and rice, the IDD gene functions of other species are largely unexplored, tomatoes are used as model plants for researching fleshy fruits, researches on tomato IDD family genes are freshly reported, and the biological functions of SlIDD genes in tomatoes are not analyzed. Therefore, the biological functions of the tomato IDD genes are explored, so that the regulation and control mechanism of the IDD family genes in fruit development and environmental response can be clarified, and an important theoretical basis is provided for quality improvement and molecular breeding of tomatoes and other fleshy fruits. Disclosure of Invention The invention aims to solve the technical problem of providing an application of SlIDD gene in improving tomato yield and sugar content in a high-temperature environment so as to solve the technical problem that tomato yield and quality can not be cooperatively improved in the high-temperature environment. In order to achieve the aim, the technical scheme adopted by the invention is that the SlIDD gene is applied to improving the yield and sugar content of tomatoes in a high-temperature environment, the tolerance of tomato plants to high temperature is improved by inhibiting SlIDD gene expression or knocking out SlIDD gene, the fruit setting rate and single plant yield of tomatoes are improved, the sucrose, fructose and glucose content of tomato fruits is improved, and the nucleotide sequence of SlIDD gene is shown as SEQ ID NO. 1. Based on the technical scheme, the invention can also be improved as follows: further, the high temperature is 30-35 ℃. Further, the amino acid sequence of the protein encoded by SlIDD gene is shown as SEQ ID NO. 2. The invention has the beneficial effects that the endogenous gene SlIDD of the tomato is knocked out in the tomato by using the CRISPR/Cas9 gene editing technology, and the phenotype identification analysis shows that the fruit setting rate of the strain with the knocked-out SlIDD gene is obviously improved under the high-temperature condition, the single plant yield is obviously improved, and the sugar content of sucrose, fructose, glucose and the like of tomato fruits in the knocked-out strain is obviously increased, so that the quality of the fruits is improved, and important gene resources and strategies are provided for cooperatively improving the yield and the quality of the tomato fruits under the high-temperature adverse condition. Drawings FIG. 1 is a constructed BG-plant-fast-cas9 expression vector map; FIG. 2 shows the results of electrophoretic detection of SlIDD gene CRISPR/Cas9 knockout plants; FIG. 3 is a schematic diagram of gene editing of SlIDD gene knockout lines; FIG. 4 is a plant status diagram of wild type and SlIDD7 knockout lines under high temperature conditions; FIG. 5 is a graph showing comparison of the number of individual results of wild type and SlIDD7 knockout lines under high temperature conditions; FIG. 6 is a statistical graph of individual fruit setting rates of wild type and SlIDD7 knockout lines under high temperature conditions; FIG. 7 is a statistical plot of fruit yields of wild type and SlIDD a 7 knockout lines under high temperature conditions; FIG. 8 is a statistical plot of sucrose content of tomato fruits under high temperature conditions for wild type and SlIDD7 knockout lines; FIG. 9 is a statistical plot of tomato fruit fructose content under high temperature conditions for wild type and SlIDD7 knockout lines; FIG. 10 is a statistical plot of tomato fruit glucose content under high temperature conditions for wild type and SlIDD7 knockout lines. Detailed Description The following description of the specific embodiments of the present invention is provided to facilitate understanding of the present invention by those skilled in the art, and the examples a