CN-122012531-A - Application of apple pattern recognition receptor gene MdRE02 in regulation and control of plant disease resistance

Abstract

The invention belongs to the technical field of genetic engineering, and relates to application of an apple pattern recognition receptor gene MdRE02 in regulating and controlling plant disease resistance. The nucleotide sequence of the apple pattern recognition receptor gene MdRE is shown as SEQ ID NO.1, the MdRE gene is silenced in apples, the immune gene expression induced by the pathogen-associated molecular pattern VmE02 is obviously reduced, and the disease resistance to apple tree canker is obviously weakened. Overexpression of MdRE gene in tobacco mutant re02 restored VmE 02-induced cell necrosis and reactive oxygen species bursts, indicating MdRE02 is a pattern recognition receptor that recognizes VmE 02. Over-expression MdRE of the gene MdRE in tobacco mutant re02 and rape can significantly increase its resistance to sclerotinia. The result shows that MdRE gene can be used for disease-resistant breeding and disease prevention and control of apple tree rot germ and sclerotinia sclerotiorum.

Inventors

• HUANG LILI
• LIU JIANYING
• ZHU LIANGLIANG

Assignees

• 西北农林科技大学深圳研究院

Dates

Publication Date

20260512

Application Date

20260413

Claims (10)

1. 1. The application of the apple pattern recognition receptor gene MdRE in regulating and controlling plant disease resistance is characterized in that the nucleotide sequence of the apple pattern recognition receptor gene MdRE is shown as SEQ ID NO.1, and the coded amino acid sequence is shown as SEQ ID NO. 2; the regulation of plant disease resistance comprises over-expressing the apple pattern recognition receptor gene MdRE to improve plant disease resistance, silencing the apple pattern recognition receptor gene MdRE to reduce plant disease resistance.
2. 2. The use of the apple pattern recognition receptor gene MdRE as claimed in claim 1 for regulating plant disease resistance, wherein the improvement of plant disease resistance is an improvement of apple resistance to apple tree canker germ VALSA MALI and/or an improvement of tobacco, rape resistance to sclerotinia sclerotiorum Sclerotinia sclerotiorum.
3. 3. The use of apple pattern recognition receptor gene MdRE as claimed in claim 1 for regulating plant disease resistance, wherein said decreasing plant disease resistance is down-regulating VmE induced apple defense-related gene expression; The apple defense related genes comprise MdPR, mdPR2, mdPR5 and MdCYP81F2.
4. 4. The application of the apple pattern recognition receptor gene MdRE in cultivating plant disease-resistant varieties is characterized in that the nucleotide sequence of the apple pattern recognition receptor gene MdRE is shown as SEQ ID NO.1, and the coded amino acid sequence is shown as SEQ ID NO. 2; The method for cultivating the plant disease-resistant variety is to obtain the plant disease-resistant variety by over-expressing the apple pattern recognition receptor gene MdRE.
5. 5. The use of apple pattern recognition receptor gene MdRE02 as claimed in claim 4 for breeding disease-resistant varieties of plants including apple, tobacco and rape.
6. 6. The use of apple pattern recognition receptor gene MdRE as claimed in claim 4 for breeding plant disease resistant varieties including apple varieties resistant to apple tree rot germ VALSA MALI, tobacco resistant to sclerotinia sclerotiorum Sclerotinia sclerotiorum and/or rape varieties.
7. 7. A method for improving disease resistance of plants is characterized by comprising the steps of constructing a transient expression vector containing apple pattern recognition receptor gene MdRE, introducing the transient expression vector into plant cells or tissues to enable apple pattern recognition receptor gene MdRE02 to be transiently over-expressed so as to improve the disease resistance of plants; the nucleotide sequence of the apple pattern recognition receptor gene MdRE is shown as SEQ ID NO. 1.
8. 8. The method of claim 7, wherein the disease resistance comprises resistance of apple to apple tree canker germ VALSA MALI and/or resistance of tobacco, rape to Sclerotinia sclerotiorum Sclerotinia sclerotiorum.
9. 9. A method for cultivating a plant disease-resistant variety is characterized by comprising the steps of constructing a transient expression vector containing an apple pattern recognition receptor gene MdRE, introducing the transient expression vector into plant cells or tissues to enable the apple pattern recognition receptor gene MdRE02 to be transiently over-expressed, and obtaining the plant disease-resistant variety; the nucleotide sequence of the apple pattern recognition receptor gene MdRE is shown as SEQ ID NO. 1.
10. 10. The method of claim 9, wherein the plant resistant varieties include apple varieties resistant to apple tree rot pathogen VALSA MALI, tobacco resistant to Sclerotinia sclerotiorum Sclerotinia sclerotiorum, and/or rape varieties.

Description

Application of apple pattern recognition receptor gene MdRE02 in regulation and control of plant disease resistance Technical Field The invention belongs to the technical field of genetic engineering, and relates to application of an apple pattern recognition receptor gene MdRE02 in regulating and controlling plant disease resistance. Background Plants are subjected to various biotic stresses during their growth, and the plants have evolved an innate immune system in order to combat the infestation by pathogenic microorganisms. Among them, pattern recognition receptors (Pattern Recognition Receptors, PRRs) located on the cell membrane are able to sense pathogen-associated molecular patterns (Pathogen-associated molecular patterns, PAMPs) and thereby activate basic immunity (Pattern-TRIGGERED IMMUNITY, PTI). PTI is used as a primary defense line for plant immunization, and can enhance the basic resistance of plants to various pathogens. Therefore, crop disease prevention and control by utilizing PTI mechanism is one of the important ways to realize environment-friendly plant protection strategy. PRRs are widely found in plants and are largely classified into Receptor-like kinases (RLK) and Receptor-like proteins (RLP) depending on the presence or absence of intracellular kinase domains. Among them, PRRs that are extracellular Leucine Rich Repeats (LRR) are the most abundant. They recognize PAMPs through the extracellular LRR domain, triggering a series of basal defense responses such as bursts of reactive oxygen species, callose deposition, and defense gene expression. Studies show that the overexpression or trans-species transfer of PRR can endow plants with broad-spectrum disease resistance, so that the PRR becomes a key target for genetic improvement of crop disease resistance. However, currently known disease-resistant related PRRs are mostly derived from model plants, few genes of the same type are identified in crops, and studies on genetic improvement based on crop endogenous PRR genes are still limited. Apples are used as important commercial crops, high disease resistance varieties in germplasm resources of apples are relatively deficient, and excavation and utilization of disease resistance genes of apples are urgent needs for genetic improvement. Since 2004 first cloned disease resistance gene HcrVf encoding LRR-RLP from apple, the excavation of other disease resistance related RLP genes in apple was relatively slow. Therefore, the identification and functional analysis of the pattern recognition receptor involved in immune regulation in apples can not only provide key gene resources for creating disease-resistant germplasm of apples, but also expand disease-resistant functions of apples to other crops through gene transfer, superposition or synthetic biological strategies, and provide important gene elements for cross-species disease-resistant breeding. Disclosure of Invention Aiming at the technical problems and defects, the invention provides application of the apple pattern recognition receptor gene MdRE to regulation and control of plant disease resistance. In a first aspect, the invention provides an application of apple pattern recognition receptor gene MdRE in regulating plant disease resistance, wherein the nucleotide sequence of apple pattern recognition receptor gene MdRE is shown as SEQ ID NO.1, and the coded amino acid sequence is shown as SEQ ID NO. 2; the regulation of plant disease resistance comprises over-expressing the apple pattern recognition receptor gene MdRE to improve plant disease resistance, silencing the apple pattern recognition receptor gene MdRE to reduce plant disease resistance. Further, in the application of the apple pattern recognition receptor gene MdRE02 provided by the invention in regulation of plant disease resistance, the improvement of plant disease resistance is to improve the resistance of apples to apple tree rot pathogen VASLA MALI and/or improve the resistance of tobacco and rape to sclerotinia sclerotiorum Sclerotinia sclerotiorum. Further, in the application of the apple pattern recognition receptor gene MdRE02 provided by the invention in regulating and controlling plant disease resistance, the reduction of plant disease resistance is to enable the expression of apple defense related genes induced by VmE02 to be down-regulated; The apple defense related genes comprise MdPR, mdPR2, mdPR5 and MdCYP81F2. In a second aspect, the invention provides application of apple pattern recognition receptor gene MdRE in cultivating plant disease-resistant varieties, the nucleotide sequence of apple pattern recognition receptor gene MdRE02 is shown as SEQ ID NO.1, and the coded amino acid sequence is shown as SEQ ID NO. 2; The method for cultivating the plant disease-resistant variety is to obtain the plant disease-resistant variety by over-expressing the apple pattern recognition receptor gene MdRE. Further, in the application of the apple pattern recognition re