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CN-122012532-A - Application of OsRPP gene in regulation and control of heat resistance and photosynthetic property of rice

CN122012532ACN 122012532 ACN122012532 ACN 122012532ACN-122012532-A

Abstract

The invention discloses an application of OsRPP gene in regulating heat resistance and photosynthetic property of rice, which is characterized in that a CRISPR-Cas9 vector is constructed, and a method of agrobacterium-mediated transformation is utilized to obtain rice OsRPP gene knockout mutant strains (osrpp-2, osrpp-13-18 and osrpp-20). The inventor reveals the function of OsRPP gene in regulating and controlling the temperature tolerance and photosynthesis performance of rice plant through raising culture temperature simulating experiment. Through the research, gene resources are provided for rice high-temperature-resistant high-light-efficiency molecular breeding. Can enable rice to survive and grow in a temperature range beyond the original adaptation range, improves photosynthetic efficiency, and provides gene resources for high-light-efficiency molecular breeding and genetic improvement of rice.

Inventors

  • WANG KE
  • ZHANG QIULI
  • ZHANG LIXIN
  • WANG LEI
  • DUAN BING
  • XU XIUMEI
  • LV DANDAN
  • SUN YANG
  • CHEN LICHAO
  • SHI YANYUN
  • LIU JIE

Assignees

  • 河南大学三亚研究院
  • 河南大学

Dates

Publication Date
20260512
Application Date
20260415

Claims (8)

  1. The application of the OsRPP13 gene in regulating heat resistance and photosynthetic performance of rice is characterized in that the nucleotide sequence of the OsRPP gene is shown as SEQ ID NO.1, and the regulation is shown as that the heat resistance and photosynthetic efficiency of the rice are improved after the expression of OsRPP gene is reduced.
  2. 2. The use according to claim 1, wherein the manner of reducing the expression of OsRPP gene is to knock out OsRPP gene.
  3. 3. The use according to claim 2, characterized in that by constructing the CRISPR-Cas9 vector of OsRPP gene, a OsRPP gene knockout mutant strain is obtained with higher heat resistance and photosynthetic efficiency than the wild type.
  4. 4. The method of claim 3, wherein the heat resistance and photosynthetic property are expressed by that the survival rate and Fv/Fm, Y (I), Y (II), ETR (I) and ETR (II) of OsRPP gene knockout mutant are higher than that of wild type under high temperature stress.
  5. 5. A plant breeding method characterized in that the method is (1) or (2) below: (1) By reducing the activity of OsRPP protein in the target plant, a plant with heat resistance and photosynthetic efficiency stronger than those of the target plant is obtained; (2) By reducing the expression of OsRPP gene in the target plant, obtaining the plant with heat resistance and photosynthetic efficiency stronger than the target plant; The target plant is rice, the amino acid sequence of OsRPP protein is shown as SEQ ID NO.2, and the nucleotide sequence of OsRPP gene is shown as SEQ ID NO. 1.
  6. 6. The method of plant breeding according to claim 5, wherein the means for reducing OsRPP gene expression is knockout OsRPP 13.
  7. 7. A method for improving heat resistance and photosynthetic performance of rice is characterized in that OsRPP gene is knocked out, and the nucleotide sequence of OsRPP gene is shown as SEQ ID NO. 1.
  8. 8. The plant breeding method according to claim 6 or the method for improving heat resistance and photosynthetic performance of rice according to claim 7, wherein OsRPP gene knockout mutant strain is obtained by using agrobacteria-mediated CRISPR-Cas9 gene editing technology.

Description

Application of OsRPP gene in regulation and control of heat resistance and photosynthetic property of rice Technical Field The invention belongs to the technical field of biology, and particularly relates to application of OsRPP gene in regulating heat resistance and photosynthetic performance of rice, and more particularly relates to application of reducing OsRPP gene expression in improving heat resistance and photosynthetic efficiency of rice. Background The environmental temperature is used as a core abiotic regulatory factor for plant growth and development, directly determines the geographical distribution, growth cycle and final yield of crops, and is one of key constraint factors affecting sustainable production of agriculture and forestry worldwide. Under high temperature stress, plants often exhibit reduced seed germination, stunted vegetative and reproductive growth, and in severe cases, lead to leaf wilting and even death. With the trend of global warming, the occurrence frequency, duration and intensity of high-temperature disasters are all in a significant rising state. The evaluation report of the united nations committee on climate change specialization shows that by the end of this century, global temperatures are expected to rise by 2.6 ℃ to 4.8 ℃. The continuously-increased environmental temperature forms a serious threat to crop yield, so that the analysis of the rice high-temperature stress tolerance mechanism is beneficial to scientific breeding of high-temperature resistant crops, and has important strategic significance for coping with climate change and guaranteeing grain safety. Rice (Oryza sativa L.) is used as staple food for more than half of the population worldwide, and its growth and development are highly sensitive to temperature change, and the suitable growth temperature range is only 20-32 ℃. High temperature stress can induce a series of physiological and biochemical and molecular disturbance throughout the whole growth period of rice, and limit the yield potential. Therefore, the molecular mechanism of the rice responding to the high temperature stress is deeply analyzed, and the key heat-resistant regulation and control genes are excavated, so that the method has important theoretical and application values for cultivating the high temperature resistant rice variety by a molecular breeding technology, improving the adaptability of crops to climate change and guaranteeing the safe production of grains. Photosynthesis is the basis for the production and yield formation of rice material, with over 90% of the dry matter required for rice yield formation resulting from the accumulation of photosynthetic products. Photosynthesis is more susceptible to high temperature stress inhibition than other physiological processes, with photosystem II (PSII) being the most sensitive core of the photosynthesis machinery. At the same time, the non-photochemical quenching capacity is reduced, the heat dissipation efficiency is reduced, and the excessive light energy accumulation induces light inhibition, and finally, the carbon assimilation capacity is reduced and the photosynthetic efficiency is reduced. The method for excavating the photosynthetic adaptability key genes of the rice under high temperature stress and analyzing the molecular mechanism thereof is an important way for improving the high temperature stress tolerance of the rice. Plants develop complex stress response mechanisms during long-term evolution, in which disease-resistant proteins (R proteins) serve as core members of the plant immune system, playing a key role in combating pathogenic bacterial invasion. The RPP13 protein is a key member of the plant immune system, and is used as a typical CC-NBS-LRR type disease-resistant protein to initiate defense by recognizing specific pathogen effector. At present, the research of RPP13 family genes is mainly focused on the disease-resistant fields of arabidopsis, tobacco and other mode plants and wheat, corn and other crops, and has proved that the gene has important functions in resisting the infection of pathogenic bacteria such as downy mildew, rice blast, bacterial leaf blight and the like. However, with the intensive research of plant adversity physiology, more and more evidence shows that part of R protein not only participates in biotic stress response, but also can participate in tolerance regulation of abiotic stress through a cross regulation mechanism-for example, CC-NBS-LRR type disease-resistant proteins such as RPM1 of arabidopsis thaliana, rp1 of corn and the like are found to respond to drought, salt stress and oxidation stress. However, up to now, research on the rice OsRPP gene (RAP-DB No.: os08g 0543100) is still limited to the annotation of disease-resistant functions, and no disclosure has been reported to determine whether the gene participates in the tolerance regulation of temperature stress, and the effect, molecular mechanism and application potentia