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CN-122012534-A - PROSiCAPE14 gene and application of coded protein thereof in improving salt tolerance of plants

CN122012534ACN 122012534 ACN122012534 ACN 122012534ACN-122012534-A

Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly relates to PROSiCAPE gene and application of coding protein thereof in improving salt tolerance of plants. The invention discovers for the first time that salt stress can lead to down-regulation of gene expression of millet PROSiCAPE. Under the salt treatment condition, the plant height and root length of the small peptide coded by PROSiCAPE gene on the outside of millet and the construction of PROSiCAPE gene over-expression strain are obviously different from those of a control group, and the plant height and root length of PROSiCAPE gene silencing plants are obviously better than those of the control group. The PROSiCAPE gene is shown to play an important role in the process of responding to salt stress in millet, and provides a basis for further researching the role of CAPE gene in the adaptation of millet to abiotic stress and other possible biological functions.

Inventors

  • SUI NA
  • GUO ZHENGYU
  • Chen Zengting

Assignees

  • 山东师范大学

Dates

Publication Date
20260512
Application Date
20260416

Claims (10)

  1. The application of PROSiCAPE14 gene and its coded protein in improving plant salt tolerance is characterized in that the coded protein is a protein shown in the following (a 1) or (a 2): (a1) A protein consisting of an amino acid sequence shown as SEQ ID NO. 1 in a sequence table; (a2) A fusion protein obtained by ligating the N-terminal and/or C-terminal of the protein defined in (a 1) with a protein tag.
  2. 2. The use according to claim 1, wherein the PROSiCAPE gene has the sequence shown in SEQ ID NO. 2.
  3. 3. The use of claim 1, wherein said use is effected by inhibiting PROSiCAPE gene expression, silencing, or knocking out PROSiCAPE gene.
  4. 4. The use of claim 3, wherein said inhibiting PROSiCAPE gene expression, silencing, or knocking out PROSiCAPE gene comprises a nucleic acid molecule that deletes or inactivates said PROSiCAPE gene by gene knockdown techniques, gene editing techniques, and/or gene knockdown techniques.
  5. 5. The use of claim 4, wherein the nucleic acid molecule comprises: (1) Double-stranded RNA, small interfering RNA, microRNA, and short hairpin RNA used in RNA interference techniques; (2) Antisense RNA and antisense oligonucleotides used in antisense nucleic acid technology; (3) gRNA and sgRNA used in gene editing techniques; (4) An aptamer and a ribozyme.
  6. 6. The use according to claim 5, wherein the antisense oligonucleotide has the sequence shown in SEQ ID NO. 3.
  7. 7. The use according to claim 1, wherein the plant is selected from at least one of rice, maize, soybean, sunflower, sorghum, wheat, alfalfa, cotton, barley, millet and millet.
  8. 8. A method for improving plant salt tolerance, which is characterized by comprising the steps of improving plant salt tolerance by inhibiting plant endogenous PROSiCAPE gene expression, silencing or knocking out plant endogenous PROSiCAPE gene, wherein the sequence of PROSiCAPE gene is shown as SEQ ID NO. 2.
  9. 9. The method of claim 8, wherein the plant is selected from at least one of rice, corn, soybean, sunflower, sorghum, wheat, alfalfa, cotton, barley, millet, and millet.
  10. 10. Use of the method of any one of claims 8 to 9 in plant breeding.

Description

PROSiCAPE14 gene and application of coded protein thereof in improving salt tolerance of plants Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to PROSiCAPE gene and application of coding protein thereof in improving salt tolerance of plants. Background Millet is a small diploid C4 spike crop, has the advantages of high water utilization efficiency, less nutrient demand, wide adaptability and the like, deeply analyzes a salt tolerance mechanism of the millet, and has important significance for utilizing marginal lands and cultivating new varieties of salt tolerant crops. In recent years, numerous researchers have employed various methods to reveal the response mechanism of millet under salt stress. For example, overexpression of millet gene SiMBR in Arabidopsis thaliana can effectively reduce in vivo active oxygen accumulation and promote drought tolerance, thereby enhancing plant stress tolerance. Wang et al found that multiple SiAAAP genes in millet were likely involved in salt stress response by transcriptome analysis and qRT-PCR techniques. However, compared with rice, arabidopsis and other mode plants, the study of the salt tolerance mechanism of millet is still in a starting stage, the number of identified genes is limited, and a complex regulation network needs to be deeply analyzed. Disclosure of Invention Aiming at the defects of the prior art, the invention aims at the application of PROSiCAPE gene and coded protein thereof in improving the salt tolerance of plants. In order to achieve the above purpose, the present invention adopts the following technical scheme: In a first aspect, the invention provides PROSiCAPE gene and the application of the coded protein in improving the salt tolerance of plants, wherein the coded protein is a protein shown as the following (a 1) or (a 2): (a1) A protein consisting of an amino acid sequence shown as SEQ ID NO. 1 in a sequence table; (a2) A fusion protein obtained by ligating the N-terminal and/or C-terminal of the protein defined in (a 1) with a protein tag. In a second aspect of the invention, there is provided the use of a biomaterial comprising the PROSiCAPE gene for modulating salt tolerance of millet. In a third aspect of the invention, a method for preparing a transgenic plant is provided, which comprises inhibiting the expression, silencing or knocking out the plant endogenous PROSiCAPE gene PROSiCAPE gene, wherein the nucleotide sequence of the PROSiCAPE gene is shown as SEQ ID NO. 2. In a fourth aspect, the invention provides a method for improving plant salt tolerance, which comprises the steps of inhibiting plant endogenous PROSiCAPE gene expression, silencing or knocking out plant endogenous PROSiCAPE gene, wherein the nucleotide sequence of PROSiCAPE gene is shown as SEQ ID NO. 2. In a fifth aspect of the invention, PROSiCAPE gene and its coding protein or biological material containing its PROSiCAPE gene or the application of the above method in plant breeding, the sequence of PROSiCAPE gene is shown in SEQ ID NO. 2. In a sixth aspect of the invention, PROSiCAPE gene and its coding protein or biological material containing its PROSiCAPE gene or the application of the above method in genetic improvement of plant germplasm resources, the sequence of PROSiCAPE gene is shown in SEQ ID NO. 2. Compared with the prior art, the technical scheme of the invention has the following beneficial effects: The invention discovers for the first time that salt stress can lead to down-regulation of gene expression of millet PROSiCAPE. Under the salt treatment condition, the plant height and root length of the small peptide coded by PROSiCAPE gene on the outside of millet and the construction of PROSiCAPE gene over-expression strain are obviously different from those of a control group, and the plant height and root length of PROSiCAPE gene editing plants are obviously better than those of the control group. The PROSiCAPE gene is shown to play an important role in the process of responding to salt stress in millet, and provides a basis for further researching the role of CAPE gene in the adaptation of millet to abiotic stress and other possible biological functions. Drawings FIG. 1 is a diagram showing the analysis of PROSiCAPE gene family expression patterns. FIG. 2 shows analysis of the CAPE gene family expression levels of millet under different salt stresses in the present embodiment, wherein A is PROSiCAPE, B is PROSiCAPE, C is PROSiCAPE, D is PROSiCAPE, E is PROSiCAPE, F is PROSiCAPE, G is PROSiCAPE, H is PROSiCAPE, and I is PROSiCAPE. FIG. 3 shows the subcellular localization of PROSiCAPE protein in tobacco according to the example of the present invention, wherein A is the subcellular localization of PROSiCAPE protein, and B is the yeast single impurity self-activation activity assay. FIG. 4 shows the effect of exogenous spray SiCAPE14 on millet growth and salt tolerance in the example of