Search

CN-122012546-A - Application of sfCHS gene in spodoptera frugiperda control and regulation of spodoptera frugiperda diet membrane

CN122012546ACN 122012546 ACN122012546 ACN 122012546ACN-122012546-A

Abstract

The invention provides an application of sfCHS gene in spodoptera frugiperda control and regulation of spodoptera frugiperda edible films, and relates to the technical field of biology. The inventor researches show that sfCHS gene participates in the synthesis of the perifasted membrane chitin in the intestinal tract, the perifasted membrane of spodoptera frugiperda disappears after the sfCHS gene is knocked out, and high fitness cost is caused when corn leaves are fed, so that sfCHS gene is an important prevention and control action target, can be used as a target gene of RNA pesticide, and the sfCHS gene is knocked out and can be used for preventing and controlling spodoptera frugiperda.

Inventors

  • XIAO YUTAO
  • WANG PENG
  • JIN MINGHUI
  • LIU ZHENXING
  • KANG QIANGQIANG

Assignees

  • 崖州湾国家实验室
  • 中国农业科学院深圳农业基因组研究所(岭南现代农业科学与技术广东省实验室深圳分中心)

Dates

Publication Date
20260512
Application Date
20260213

Claims (10)

  1. The application of the sfCHS2 gene in spodoptera frugiperda control is characterized in that the nucleic acid sequence of the sfCHS gene is shown as SEQ ID NO. 1; The sfCHS gene of spodoptera frugiperda is knocked out to realize the control of spodoptera frugiperda.
  2. 2. The use of claim 1, wherein the sfCHS gene is knocked out using the CRISPR/Cas9 system.
  3. 3. The use according to claim 2, wherein the target sequence of the sgRNA of the CRISPR/Cas9 system is as shown in SEQ ID No. 2.
  4. 4. The use according to claim 3, characterized in that the sequence of the sgRNA is shown in SEQ ID No. 3.
  5. 5. The use according to claim 4, wherein after said sfCHS gene knockout, spodoptera frugiperda is obtained in which the nucleic acid sequence of exon 6 TAAAGAAGACTCGTTACTACACA of sfCHS gene is deleted.
  6. The application of the sfCHS2 gene in regulating and controlling spodoptera frugiperda edible films is characterized in that the nucleic acid sequence of the sfCHS gene is shown as SEQ ID NO. 1; The sfCHS gene of spodoptera frugiperda is knocked out, and the peri-feeding membrane of spodoptera frugiperda is deleted.
  7. 7. The use of claim 6, wherein the sfCHS gene is knocked out using the CRISPR/Cas9 system.
  8. 8. The use according to claim 7, wherein the target sequence of the sgRNA of the CRISPR/Cas9 system is as shown in SEQ ID No. 2.
  9. 9. The use according to claim 8, wherein the sgRNA has the sequence shown in SEQ ID No. 3.
  10. 10. The use according to claim 9, wherein after said sfCHS gene knockout, spodoptera frugiperda is obtained in which the nucleic acid sequence of exon 6 TAAAGAAGACTCGTTACTACACA of the sfCHS gene is deleted.

Description

Application of sfCHS gene in spodoptera frugiperda control and regulation of spodoptera frugiperda diet membrane Technical Field The invention relates to the technical field of biology, in particular to application of sfCHS gene in spodoptera frugiperda control and regulation of spodoptera frugiperda surrounding edible film. Background Spodoptera frugiperda (Spodoptera frugiperda) belongs to the genus spodoptera of the family spodopteraceae, commonly known as fall armyworm. The species is native to tropical and subtropical areas in america, and by virtue of the biological characteristics of wide host range, strong adaptability, outstanding flying ability and high fertility, the species can rapidly generate pesticide resistance, thereby forming a serious threat to agricultural production. The current main means for preventing and controlling spodoptera frugiperda is chemical prevention and control, and high-strength pesticide spraying is easy to cause the improvement of the resistance of pests, pesticide residues and environmental pollution. Development of new gene targets will enable more accurate and efficient pest control technology development. In view of this, the present invention has been made. Disclosure of Invention The first object of the present invention is to provide the use of sfCHS (spodoptera frugiperda chitin synthase 2) gene in spodoptera frugiperda control. The second object of the invention is to provide an application of sfCHS gene in regulating and controlling spodoptera frugiperda periwinkle membrane. In order to achieve the above object, the following technical scheme is adopted: In a first aspect, the invention provides an application of sfCHS gene in spodoptera frugiperda control, wherein the nucleic acid sequence of sfCHS gene is shown as SEQ ID NO. 1; The sfCHS gene of spodoptera frugiperda is knocked out to realize the control of spodoptera frugiperda. As a further technical scheme, the sfCHS gene is knocked out by using a CRISPR/Cas9 system. As a further technical scheme, the target sequence of the sgRNA of the CRISPR/Cas9 system is shown as SEQ ID NO. 2. As a further technical scheme, the sequence of the sgRNA is shown as SEQ ID NO. 3. As a further technical scheme, after sfCHS gene knockout, spodoptera frugiperda with the sfCHS gene No. 6 exon TAAAGAAGACTCGTTACTACACA nucleic acid sequence deleted is obtained. In a second aspect, the invention provides an application of sfCHS gene in regulating and controlling spodoptera frugiperda periplaneta, wherein the nucleic acid sequence of sfCHS gene is shown as SEQ ID NO. 1; The sfCHS gene of spodoptera frugiperda is knocked out, and the peri-feeding membrane of spodoptera frugiperda is deleted. As a further technical scheme, the sfCHS gene is knocked out by using a CRISPR/Cas9 system. As a further technical scheme, the target sequence of the sgRNA of the CRISPR/Cas9 system is shown as SEQ ID NO. 2. As a further technical scheme, the sequence of the sgRNA is shown as SEQ ID NO. 3. As a further technical scheme, after sfCHS gene knockout, spodoptera frugiperda with sfCHS gene exon 6 TAAAGAAGACTCGTTACTACACA (SEQ ID NO. 4) nucleic acid sequence deleted is obtained. Compared with the prior art, the invention has the following beneficial effects: The inventor researches show that sfCHS gene participates in the synthesis of the perifasted membrane chitin in the intestinal tract, the perifasted membrane of spodoptera frugiperda disappears after the sfCHS gene is knocked out, and high fitness cost is caused when corn leaves are fed, so that sfCHS gene is an important prevention and control action target, can be used as a target gene of RNA pesticide, and the sfCHS gene is knocked out and can be used for preventing and controlling spodoptera frugiperda. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art. FIG. 1: screening procedure for SfCHS-KO homozygous line after CRISPR/Cas9 pair SfCHS gene knockout; FIG. 2 CRISPR/Cas9 mediated spodoptera frugiperda sfCHS gene knockout, mutation of SfCHS2 occurred in exon 6, bolded as sgRNA target sequence, black underlined base as original spacer adjacent motif (PAM site), black dash represents base deletion compared to Wild Type (WT) sequence, number in brackets is number of deleted bases; FIG. 3 midgut cross-sectional slices after feeding non-transgenic corn leaves with spodoptera frugiperda wild Sf-WT and knockout line SfCHS-KO end-instar larvae; FIG. 4 survival curves of spodoptera frugiperda wild type Sf-WT and knock-out strain SfCHS-KO first-instar larvae (A) and sixth-instar larvae (B) fed n