CN-122012556-A - Plant bright leaf character related gene BrTRUD, molecular marker and application thereof

CN122012556ACN 122012556 ACN122012556 ACN 122012556ACN-122012556-A

Abstract

The invention belongs to the technical field of genetic engineering, and particularly relates to a plant bright leaf character related gene BrTRUD, a molecular marker and application thereof, wherein the nucleotide sequence of BrTRUD is shown as SEQ ID NO. 26. The invention identifies that one base transversion of the promoter region is the cause of wdm21 brightness She Biaoxing through genetic localization and KASP genotyping analysis, and the mutation is located in the promoter region of pseudouridine synthetase gene BrTRUD 1. The result of the invention reveals a novel function of BrTRUD < 1 >, and illustrates that the gene plays a key role in the formation of wax crystals of the epidermis of the Chinese cabbage.

Inventors

LIU ZHIYONG
ZHAO NINGQI
FENG HUI
YE XUELING
ZHANG YUN
YANG GUOLIANG
TAN LI
WANG NAN
HUANG SHENGNAN

Assignees

沈阳农业大学

Dates

Publication Date: 20260512
Application Date: 20260319

Claims (8)

1. A plant bright leaf character related gene BrTRUD1 is characterized in that the nucleotide sequence of BrTRUD is shown as SEQ ID NO. 26.
2. The application of claim BrTRUD1, wherein the application is: The expression level of BrTRUD < 1 > in the plant is reduced to reduce the alkane content, thereby obtaining the plant with bright leaf character.
3. The use according to claim 2, wherein the plant comprises any one of chinese cabbage and arabidopsis thaliana.
4. A molecular marker related to plant bright leaf characters is characterized in that the molecular marker is positioned in a promoter region BrTRUD in claim 1, and the specific position of the molecular marker is 270bp upstream of a start codon; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the nucleotide at 101bp is A or G.
5. A KASP primer for identifying the molecular marker of claim 4, wherein the sequence of the KASP primer is shown in SEQ ID NO.13 and SEQ ID NO. 14.
6. The use of a molecular marker according to claim 4 and/or a KASP primer according to claim 5, wherein the use is any one of the following: 1) Identifying or aiding in identifying the bright leaf trait of the plant; 2) Screening or breeding plant lines or varieties with bright leaf characters; 3) And (5) preparing a product for identifying the bright leaf character of the plant.
7. The use according to claim 6, wherein the method for identifying the bright leaf trait of a plant comprises: Extracting genome DNA of a plant to be detected; detecting the genotype of 101 th position of the molecular marker; if the genotype is AA, judging that the plant to be tested has bright leaf character.
8. The use according to claim 6, wherein the plant is chinese cabbage.

Description

Plant bright leaf character related gene BrTRUD, molecular marker and application thereof Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to a plant bright leaf character related gene BrTRUD, a molecular marker and application thereof. Background Cabbage (Brassica rapal. Ssp. Pekinensis) is a major vegetable crop in the east asia region. Blade gloss is an important factor in determining its marketability. The bright She Baicai has gained increased consumer popularity due to its bright, shiny appearance resulting from the reduced wax on the skin. The light She Biaoxing is attractive in appearance and also helps to remove pesticide residues during cleaning. Therefore, the breeding of bright leaf varieties has become a new goal of cabbage breeding. Bright She Biaoxing is closely related to the composition and structure of waxy crystals of the plant cuticle, which is a hydrophobic barrier covering the surface of the plant's aboveground organs. The wax of the epidermis is a complex mixture of very long chain fatty acids and their derivatives, such as alkanes, alcohols, aldehydes and esters, which constitute the primary barrier against non-stomatal moisture loss, uv radiation and pathogenic bacteria invasion. Forward genetic screening and natural mutation analysis by ethyl methylsulfonate mutagenesis have become effective means for identifying key genes for waxy synthesis. To date, a large number of waxy defect mutants have been identified in a number of species. In Arabidopsis, CER1, CER3 and WIN1/SHN1 isogenes have been cloned and partially homologous genes such as CsCER in cucumber and OsGL in rice have also been identified. In brassica crops, genes controlling epidermal waxy biosynthesis are gradually revealed. For example BoCgl2 was identified as a gene that resulted in the bright leaf trait of Brassica oleracea L.var.capitata. In young laver (Brassica rapal. Var. Purdurea), brCER4 is responsible for the biosynthesis of cutin wax, and its mutation results in bright She Biaoxing. Furthermore, brMYB and BrWAX2 were also found to be associated with the accumulation of wax on the cabbage cuticle. Therefore, development of a gene related to cabbage light She Biaoxing is urgently required. Disclosure of Invention The invention aims to provide a plant bright leaf character related gene BrTRUD1, which solves the problems existing in the prior art. The technical scheme adopted by the invention is as follows: The invention provides a plant bright leaf character related gene BrTRUD, wherein the nucleotide sequence of BrTRUD is shown as SEQ ID No. 26. The second aspect of the present invention provides an application of BrTRUD a, where the application refers to: The expression level of BrTRUD < 1 > in the plant is reduced to reduce the alkane content, thereby obtaining the plant with bright leaf character. Preferably, the plant includes any one of chinese cabbage and arabidopsis thaliana. The third aspect of the invention provides a molecular marker related to plant bright leaf characters, wherein the molecular marker is positioned in a promoter region of BrTRUD < 1 >, and the specific position of the molecular marker is 270bp upstream of a start codon; The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the nucleotide at 101bp is A or G. In a fourth aspect, the invention provides a KASP primer for identifying the molecular marker, wherein the sequence of the KASP primer is shown in SEQ ID NO.13 and SEQ ID NO. 14. In a fifth aspect, the present invention provides an application of the molecular marker and/or the KASP primer, wherein the application refers to any one of the following: 1) Identifying or aiding in identifying the bright leaf trait of the plant; 2) Screening or breeding plant lines or varieties with bright leaf characters; 3) And (5) preparing a product for identifying the bright leaf character of the plant. Preferably, the method for identifying the bright leaf trait of a plant is as follows: Extracting genome DNA of a plant to be detected; detecting the genotype of 101 th position of the molecular marker; if the genotype is AA, judging that the plant to be tested has bright leaf character. Preferably, the plant is chinese cabbage. Compared with the prior art, the invention has the beneficial effects that: The invention provides a plant bright leaf character related gene BrTRUD, wherein the nucleotide sequence of BrTRUD is shown as SEQ ID No. 26. The invention separates a bright leaf mutant wdm21 from a cabbage group chemically mutagenized by EMS. By MutMap genetic mapping and KASP genotyping analysis, it was identified that one base transversion of the promoter region was responsible for wdm21 light She Biaoxing, the mutation being located in the promoter region of pseudouridine synthetase gene BrTRUD 1. The result of the invention reveals a novel function of BrTRUD < 1 >, and illustrates that the gene plays a key role