CN-122012573-A - Application of tobacco NbASL gene in improving plant antiviral property

Abstract

The invention discloses application of tobacco NbASL genes in improving plant antiviral property, belonging to the technical field of molecular biology. The nucleic acid sequence of NbASL gene is shown in SEQ ID NO. 1. Silencing NbASL genes in Nicotiana benthamiana revealed that the sensitivity of plants to Potato Virus Y (PVY) and pepper vein mottle virus (ChiVMV) was significantly enhanced and the virus accumulation was significantly increased. In contrast, overexpression of NbASL gene can significantly inhibit PVY infection and reduce viral RNA accumulation. And PVY or ChiVMV infection can induce up-regulation of NbASL gene expression in Nicotiana benthamiana. Therefore, nbASL gene is a positive regulator in plant antiviral response. The invention provides important gene resources and theoretical basis for cultivating a novel crop variety with broad spectrum and lasting antiviral property by utilizing NbASL genes, and has important application value.

Inventors

• ZHANG XIFENG
• TIAN GENG
• WANG BINGANG
• ZHANG SHIJIE
• LIU YUN
• DENG XIAOCHENG
• QIAN XINYU

Assignees

• 陕西省烟草公司宝鸡市公司

Dates

Publication Date

20260512

Application Date

20251216

Claims (10)

1. 1. The application of the tobacco NbASL gene in improving plant virus resistance is characterized in that the amino acid sequence of the tobacco NbASL gene is shown as SEQ ID NO. 2.
2. 2. The use of tobacco NbASL gene according to claim 1 for increasing plant antiviral properties, wherein the nucleic acid sequence of the tobacco NbASL gene is shown in SEQ ID No. 1.
3. 3. Use of the tobacco NbASL gene according to claim 1 or 2 for increasing plant antiviral properties, wherein the virus is potyvirus.
4. 4. Use of the tobacco NbASL gene according to claim 3 for increasing plant antiviral properties, wherein the potyvirus is potyvirus or capsicum mottle virus.
5. 5. The application of a product containing tobacco NbASL genes in improving plant virus resistance is characterized in that the amino acid sequence coded by the tobacco NbASL genes is shown as SEQ ID NO. 2.
6. 6. The use of a product comprising a tobacco NbASL gene according to claim 5 for increasing plant antiviral properties, wherein the nucleic acid sequence of the tobacco NbASL gene is shown in SEQ ID No. 1.
7. 7. Use of a product comprising the tobacco NbASL gene according to claim 5 for increasing plant antiviral properties, wherein the product is an expression vector comprising the NbASL gene, an expression cassette or a host cell comprising the expression vector.
8. 8. A method of increasing antiviral activity in a plant, comprising introducing and expressing in the plant the tobacco NbASL gene of claim 1.
9. 9. The method of increasing antiviral activity in a plant of claim 8, comprising transforming an expression vector comprising the tobacco NbASL gene into a plant.
10. 10. The method of increasing antiviral activity in a plant according to claim 8 or 9, wherein the plant is a solanaceae plant.

Description

Application of tobacco NbASL gene in improving plant antiviral property Technical Field The invention belongs to the technical field of molecular biology, and particularly relates to application of tobacco NbASL genes in improving plant virus resistance. Background Tobacco is a worldwide important commercial crop, however, in the course of its plantation production, the damage caused by viral disease is one of the key factors restricting its yield and quality improvement. Among them, potto Virus Y (PVY) is a typical member of potyvirus (Potyvirus), and is one of the most serious viral diseases damaging solanaceous crops such as tobacco and Potato. PVY is mainly transmitted through aphids in a non-persistent mode, and symptoms such as leaf and leaf necrosis, plant dwarfing and the like are caused after plant infection, so that huge economic loss is caused. In recent years, the damage of another potato genus Y virus, namely, pepper vein mottle virus (CHILLI VEINAL mottle virus, chiVMV), on solanaceae crops is also increasing, which causes mottle, shrinkage and fruit malformation of leaves, further aggravating the control pressure in production. At present, efficient and specific chemical agents are lacking in production for preventing and treating plant viruses such as PVY, chiVMV and the like. The existing prevention and control strategies mainly depend on breeding and utilizing disease-resistant varieties. However, the natural antiviral gene resources of crops are limited, and virus populations are continuously evolved, so that the disease resistance of the crops can be overcome, and the disease resistant varieties are invalid. In addition, although harm can be reduced to a certain extent through agricultural management measures (such as prevention and treatment of a toxin transmission medium, removal of a disease strain and the like), the effect is unstable and the cost is high. Therefore, the novel antiviral genes of the plants are mined, the action mechanism of the novel antiviral genes is clarified, and the novel antiviral genes have important significance for developing novel virus prevention and control strategies and cultivating broad-spectrum durable disease-resistant crop varieties. Plants form a complex set of immune systems during long term evolution to cope with pathogen infestation. Among them, some key enzymes in plants and their mediated metabolic pathways play an important role in disease-resistant defensive reactions. Therefore, the field is urgent to find a new key gene capable of effectively participating in plant antiviral reaction, and develop an application method of the key gene in antiviral breeding of rice, tobacco, tomato and other economic crops so as to overcome the defects of the existing control technology. Disclosure of Invention Aiming at the problems existing in the prior art, the invention aims to provide the application of the tobacco NbASL gene in improving plant virus resistance. In order to achieve the above purpose, the invention adopts the following technical scheme: An application of tobacco NbASL gene in improving plant virus resistance is provided, wherein the amino acid sequence of tobacco NbASL gene code is shown in SEQ ID NO. 2. Based on the scheme, the nucleic acid sequence of the tobacco NbASL gene is shown as SEQ ID NO. 1. Based on the above scheme, the virus is potyvirus. Based on the above scheme, the potyvirus is potyvirus or pepper vein mottle virus. The application of a product containing tobacco NbASL genes in improving plant virus resistance is provided, and the amino acid sequence coded by the tobacco NbASL genes is shown as SEQ ID NO. 2. Based on the scheme, the nucleic acid sequence of the tobacco NbASL gene is shown as SEQ ID NO. 1. Based on the above scheme, the product is an expression vector containing NbASL genes, an expression cassette or a host cell containing the expression vector. A method for increasing antiviral activity in a plant, the method comprising introducing and expressing in the plant a tobacco NbASL gene as described above. On the basis of the above scheme, the method comprises the step of transforming an expression vector containing the tobacco NbASL gene into a plant. Based on the above scheme, the plant body is transformed by one of agrobacterium-mediated method, gene gun method, electric shock method, PEG method and liposome method. Based on the scheme, the agrobacterium is agrobacterium tumefaciens or agrobacterium rhizogenes. On the basis of the above scheme, the plant is a Solanaceae plant, further, the plant is selected from tobacco, potato, tomato or capsicum. Advantages of the technical proposal of the invention The invention discloses the key effect of tobacco NbASL gene in resisting potato virus Y (PVY and ChiVMV in particular) for the first time, and provides a brand new and effective candidate gene for crop virus resistance breeding. Moreover, through experiments of functional deletion (silencing) and functional ac