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CN-122012574-A - Application of cinnamyl alcohol dehydrogenase CsCAD L gene of tea tree in regulation and control of anthracnose resistance of plants

CN122012574ACN 122012574 ACN122012574 ACN 122012574ACN-122012574-A

Abstract

The invention discloses an application of a tea tree cinnamyl alcohol dehydrogenase CsCAD L gene in regulating and controlling plant anthracnose resistance, and the biological function of the CsCAD L gene is verified for the first time through an over-expression experiment. Through transient over-expression CsCAD L genes in tea leaves, the resistance of tea to anthracnose can be obviously improved, and simultaneously, the resistance of tobacco to anthracnose can be obviously improved through over-expression CsCAD L genes in tobacco. Functional verification of the tea tree cinnamyl alcohol dehydrogenase CsCAD L gene can provide gene resources for breeding new varieties of tea trees. The invention provides an important gene resource which possibly has universality for regulating and controlling the anthracnose resistance of plants, and provides excellent candidate genes for cultivating anthracnose-resistant plant varieties.

Inventors

  • Lv Wuyun
  • WANG YUCHUN
  • GUAN SUZHEN
  • JIANG HONG
  • CHEN YANAN
  • Ren Hengze
  • LU MENGQIAN

Assignees

  • 浙江农林大学

Dates

Publication Date
20260512
Application Date
20251230

Claims (10)

  1. 1. An application of cinnamyl alcohol dehydrogenase CsCAD L gene of tea tree in regulating plant anthracnose resistance.
  2. 2. The use according to claim 1, wherein the plants comprise woody plants and herbaceous plants.
  3. 3. The use of claim 2, wherein the plant comprises tea tree and tobacco.
  4. 4. The use according to claim 1, wherein the nucleotide sequence of the tea tree cinnamyl alcohol dehydrogenase CsCAD L gene is shown in SEQ ID No. 1.
  5. 5. The use according to claim 1, wherein the use comprises 1) allowing the plant to contain CsCAD L genes, or 2) allowing the plant to overexpress CsCAD L genes.
  6. 6. The use according to claim 5, wherein mode 2) uses agrobacterium-mediated transformation of CsCAD L genes into the plant genome to obtain plants with transient overexpression of CsCAD L genes.
  7. 7. The use according to claim 5, wherein mode 2) the CsCAD L gene is transferred into the plant genome by leaf disc method to obtain a plant with stable over-expression of CsCAD L gene.
  8. 8. The use according to claim 1, wherein the CsCAD L gene is inserted into the BamHI and SalI sites of plant expression vector pCAMBIA2300 with 35S promoter or the XbaI and SacI sites of pCAMBIAsuper1300, and transferred into the plant genome to increase the anthracnose resistance of the plant.
  9. 9. An application of cinnamyl alcohol dehydrogenase CsCAD L gene of tea tree in improving the breeding of plant anthracnose resistance.
  10. 10. The use according to claim 9, wherein the use is overexpression of CsCAD L gene in the plant genome.

Description

Application of cinnamyl alcohol dehydrogenase CsCAD L gene of tea tree in regulation and control of anthracnose resistance of plants Field of the art The invention belongs to the technical field of biological genetic engineering, and particularly relates to application of a cinnamyl alcohol dehydrogenase CsCAD L gene of tea trees in regulation and control of anthracnose resistance of plants. (II) background art Tea tree [ CAMELLIA SINENSIS (l.) o.kuntze ] is a evergreen shrub or small arbor of the genus camellia of the family camellia with a long history, widely distributed in multiple countries, one of the oldest tree species. Tea trees are used as leaf cash crops in China, and leaf diseases can directly cause the yield and quality of tea to be reduced. Tea trees are suitable for growing in warm and humid climates, which simultaneously provides suitable environmental conditions for anthracnose to occur. Anthracnose is one of the most serious leaf fungal diseases in tea plant diseases, and it is reported that 30-50% of yield loss is caused by tea anthracnose in China every year, so that sustainable development of tea industry is seriously influenced or even restricted. Therefore, the breeding and planting of the high disease-resistant tea tree variety can reduce the harm of anthracnose to tea trees, and further improve the quality and yield of tea leaves, and has great significance. Lignin, which is an important structural component in plant cell walls, is present in the plant cell walls in an amount of about 25% to 40%, next to cellulose, and is an important phenolic polymer. The deposition of lignin in the cell wall not only enhances the mechanical supporting capability of the whole plant, but also plays an important role in protecting against pathogen invasion, drought stress and other biological and non-biological adversity. Lignin formation is achieved by the phenylpropane metabolic pathway, a process that requires the synergistic action of multiple enzymes. Cinnamyl alcohol dehydrogenase (Cinnamyl alcohol dehydrogenase, CAD) is a key enzyme in the lignin biosynthesis pathway, catalyzing NADPH-dependent reduction of hydroxycinnamaldehyde (p-coumarylaldehyde, sinapial, and coniferyl aldehyde) to the corresponding alcohols (p-coumarylalcohol, sinapyl alcohol, and coniferyl alcohol), promoting the formation of monolignol (SYRINGYL LIGNIN, S-lignin; guaiacyl lignin, G-lignin; para-hydroxy-PHENYL LIGNIN, H-lignin). The tea tree is a perennial woody plant, adopts conventional hybridization, radiation mutagenesis and other breeding methods, and has the characteristics of complex breeding program, long breeding period and low breeding efficiency. Because of the conservation of genes, different genes have different influences on lignin synthesis, so that the genes for effectively regulating and controlling the lignin synthesis of tea trees are required to be searched, and the disease resistance of the tea trees to anthracnose is improved. (III) summary of the invention The invention aims to provide an application of a tea tree cinnamyl alcohol dehydrogenase CsCAD L gene in regulating and controlling anthracnose resistance of plants. According to the invention, biological function research is performed by transiently over-expressing the gene in tea trees and stably over-expressing the gene in tobacco, so that the CsCAD L gene can improve the disease resistance of tea trees to anthrax bacteria by regulating lignin synthesis, gene resources are provided for cultivating high-anthracnose-resistance tea tree varieties, and the method has excellent potential application value. The technical scheme adopted by the invention is as follows: The invention provides an application of a tea tree cinnamyl alcohol dehydrogenase CsCAD L gene in regulating and controlling anthracnose resistance of plants. Further, the plants include woody plants and herbaceous plants. Further, the plants include tea tree and tobacco. Further, the nucleotide sequence of the tea tree cinnamyl alcohol dehydrogenase CsCAD L gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The CsCAD L gene according to the present invention encodes a protein of 144 amino acids in length, containing an alcohol dehydrogenase domain ADH_N, whose CDS sequence has been submitted to NCBI (GenBank accession number: PX 609947). Further, the use includes 1) allowing the plant to contain CsCAD L of the gene, or 2) allowing the plant to overexpress CsCAD L of the gene. Further, the mode 2) adopts an agrobacterium-mediated method to transfer CsCAD L genes into a plant genome to obtain plants with CsCAD L genes transiently over-expressed. Further, the mode 2) adopts a leaf disk method to transfer CsCAD L genes into a plant genome to obtain a plant with CsCAD L genes stably and excessively expressed. Further, the CsCAD L gene is inserted into BamHI and SalI sites of a plant expression vector pCAMBIA2300 or XbaI and SacI sit