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CN-122012577-A - Application of OsAIP gene in increasing rice yield

CN122012577ACN 122012577 ACN122012577 ACN 122012577ACN-122012577-A

Abstract

The invention relates to the technical field of plant genetic engineering, in particular to application of OsAIP gene in increasing rice yield. The invention discovers that OsAIP gene is a key gene for regulating and controlling the tillering number of rice, and the over-expression of OsAIP gene can promote the elongation speed of first tillering buds so as to increase the tillering number of the rice, so that the single plant yield of the rice is improved under the condition that the plant height and the spike number of the rice are kept unchanged. The OsAIP gene provided by the invention has important theoretical value and application prospect for high-yield breeding improvement of crops such as rice and the like.

Inventors

  • LIN RONGCHENG
  • WU XIAOKANG
  • WANG SHUMAO

Assignees

  • 湘湖实验室(农业浙江省实验室)

Dates

Publication Date
20260512
Application Date
20260129

Claims (10)

  1. Use of the osaip1 gene or a biomaterial related thereto in at least one of the following: a1 Regulating the yield of rice or preparing a product for regulating the yield of rice; a2 Cultivating rice with increased yield or preparing a product with increased rice yield; a3 Preparing transgenic rice; The nucleotide sequence of OsAIP gene comprises SEQ ID NO. 1.
  2. 2. The use of claim 1, wherein the biological material comprises one or more of the following: b1 A protein encoded by the OsAIP gene and/or a recombinant vector, recombinant microorganism or transgenic rice cell line containing the OsAIP gene; b2 Nucleic acid molecules, recombinant vectors, recombinant microorganisms or transgenic rice cell lines containing negative control OsAIP gene expression.
  3. 3. The use according to claim 1 or 2, wherein controlling rice yield comprises controlling the rate of elongation of the first tillering bud and thereby controlling the number of tillers in the rice.
  4. 4. The use according to claim 3, wherein the controlling of rice tillering number comprises increasing rice yield by positively controlling OsAIP gene expression; Alternatively, rice yield is reduced by negatively regulating OsAIP gene expression.
  5. 5. The application of the biological material for positively regulating OsAIP gene expression in rice with improved yield is disclosed, wherein the nucleotide sequence of OsAIP gene comprises SEQ ID NO. 1.
  6. 6. The method according to claim 5, wherein the biological material comprises a protein encoded by the OsAIP gene and/or a recombinant vector, recombinant microorganism or transgenic rice cell line containing positive control of OsAIP gene expression.
  7. 7. The use of claim 5, wherein the increasing rice yield comprises increasing the elongation rate of the first tillering bud to positively regulate the number of tillers in the rice.
  8. 8. A method for increasing rice yield comprising: The biological material for positively controlling OsAIP gene expression is transferred into rice.
  9. 9. A method for growing rice with increased yield comprising: The biological material for positively controlling OsAIP gene expression is transferred into rice.
  10. 10. A rice with improved yield, characterized in that the rice comprises a biological material that positively regulates OsAIP gene expression; The nucleotide sequence of OsAIP gene comprises SEQ ID NO. 1.

Description

Application of OsAIP gene in increasing rice yield Technical Field The invention relates to the technical field of plant genetic engineering, in particular to application of OsAIP gene in increasing rice yield. Background Rice (Oryza sativa l.) is an important food crop, and more than half of the world population uses rice as a staple food. The yield traits of rice are complex quantitative traits commonly controlled by multiple genes and the environment. The yield of rice consists of three factors, namely tillering number, grain number per spike and thousand grain weight. The tillering is used as a core link for the plant type construction of gramineous crops, and the effective spike number is directly determined, so that the tillering is a primary factor affecting the yield. The formation of rice tillering comprises two key stages of tillering bud initiation and tillering bud elongation, and the process is precisely regulated and controlled by a complex genetic network and various internal and external signals. In the initial stage of tillering buds, a series of key transcription factors play an indispensable role. Monoculm 1 (MOC 1) is the first cloned tillering important gene whose loss of function results in complete inhibition of axillary bud formation. Lax panicle (LAX 1) and (LAX 2) regulate normal differentiation of meristems by interaction, while Monoculm (MOC 3) also plays an important role in this process. The elongation of tillering buds is mainly controlled by the cooperation of plant hormone signal networks, including Cytokinin (CK), gibberellin (GA), brassinolide (BR) and Strigolactone (SL). In addition to genetic and hormonal regulation, nutrient elements (such as nitrogen and phosphorus) are also involved in the regulation of rice tillering. The nitrogen can promote the elongation of tillering buds by promoting the synthesis of cytokinin by root systems and directly enhancing nitrogen metabolism, and the phosphorus deficiency can inhibit tillering. Other important regulatory genes further enrich the network, for example Squamosa Promoter binding protein-Like 14 (SPL 14) transcription factors are used as multiple regulatory hinges, and can regulate and control downstream targets such as TEOSINTE BRANCHED 1 (OsTB 1) and the Like through different interacting proteins respectively, so that tillering is cooperatively inhibited and ear development is promoted. OsMADS57 promotes tillering by inhibiting expression of the SL receptor gene DWARF14 (D14), which is in turn inhibited by OsTB 1. These findings reveal the high complexity and layering of tillering control networks. Future researches need to further analyze the molecular mechanism of integration of different signal paths in the space-time dimension, directionally improve the plant type of crops in a complex field environment, and realize new breakthrough of yield. Disclosure of Invention Aiming at the defects of the prior art, the invention provides the application of OsAIP gene in increasing the yield of rice. The specific technical scheme is as follows: The present invention provides in a first aspect the use of the OsAIP gene or a biomaterial related thereto in at least one of: a1 Regulating the yield of rice or preparing a product for regulating the yield of rice; a2 Cultivating rice with increased yield or preparing a product with increased rice yield; a3 Preparing transgenic rice; The nucleotide sequence of OsAIP gene comprises SEQ ID NO. 1. Further, the biological material comprises one or more of the following: b1 A protein encoded by the OsAIP gene and/or a recombinant vector, recombinant microorganism or transgenic rice cell line containing the OsAIP gene; b2 Nucleic acid molecules, recombinant vectors, recombinant microorganisms or transgenic rice cell lines containing negative control OsAIP gene expression. Further, the regulation of the rice yield comprises regulation of the elongation speed of the first tillering bud and further regulation of the rice tillering number. Further, the regulation of the tiller number of the rice comprises the step of improving the yield of the rice by positively regulating and controlling the expression of OsAIP gene. Further, the amino acid sequence of the OsAIP gene coding protein comprises the amino acid sequence shown in SEQ ID NO. 2. Further, the recombinant vector containing OsAIP1 genes comprises a basic vector and OsAIP1, and the basic vector comprises a pCAMBIA2300 vector. Further, the recombinant microorganism containing OsAIP a gene includes E.coli DH5 a and Agrobacterium GV3101. Further, the regulation of the tiller number of the rice comprises the step of reducing the yield of the rice by negatively regulating the expression of OsAIP gene. Further, the nucleic acid molecule for negative regulation OsAIP of gene expression comprises a single guide RNA, and the nucleotide sequence of the single guide RNA comprises the nucleotide sequence shown in SEQ ID NO. 3. Further, the recombinant