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CN-122012582-A - Application of RNA binding protein/Y-motif regulation model in plant growth-resistance tradeoff

CN122012582ACN 122012582 ACN122012582 ACN 122012582ACN-122012582-A

Abstract

The invention discloses application of an RNA binding protein/Y-motif regulation model in plant growth-resistance balance, and relates to the technical fields of plant molecular biology and agricultural biology. The RNA Binding Protein (RBP) is a protein having a typical RNA Recognition Motif (RRM), and the Y-motif is an RNA element widely existing in plant growth genes. The RBP/Y-motif model is used for regulating and controlling the mRNA stability of the growth genes so as to realize the effect of plant growth-resistance balance. The method provided by the invention is not only applied to regulation and control of plant growth-resistance balance, deepens understanding of plant growth-resistance balance, but also provides brand new targets and theoretical basis for crop genetic improvement, lays a key technical foundation for cultivating disease-resistant high-yield new varieties, and can also provide reference value for research of other adversity stress.

Inventors

  • XU GUOYONG
  • ZHOU GUILONG
  • NIU RUIXIA
  • ZHU SITAO
  • WANG WANZHEN

Assignees

  • 武汉大学

Dates

Publication Date
20260512
Application Date
20260204

Claims (10)

  1. The application of an RNA binding protein/Y-motif regulation model in balancing plant resistance and yield is characterized in that the RNA binding protein/Y-motif regulation model consists of an RNA binding protein and a Y-motif element, and the structure is as follows: ; the RNA binding protein specifically recognizes and binds to the Y-motif element on plant growth gene mRNA, and regulates the expression of the plant growth gene.
  2. 2. A method for balancing plant resistance and yield is characterized in that a gene for expressing an RNA binding protein is transferred into a plant to enable the RNA binding protein to be overexpressed, and the RNA binding protein specifically recognizes and binds to the Y-motif element on plant growth gene mRNA and regulates and controls the expression of the plant growth gene during immune activation.
  3. 3. The method of balancing plant resistance and yield according to claim 2, wherein the RNA binding protein is a PTBP3 protein of arabidopsis thaliana, and the amino acid sequence of the PTBP3 protein of arabidopsis thaliana is shown in SEQ ID No. 43.
  4. 4. A method of balancing plant resistance and yield according to claim 3 wherein the nucleotide sequence encoding the PTBP3 protein of arabidopsis thaliana is shown in SEQ ID No. 37.
  5. 5. A method of balancing plant resistance and yield according to claim 3 wherein the plant growth gene is a GRF3 gene.
  6. 6. A method for regulating and controlling the expression of plant growth genes is characterized in that gene segments expressing RNA binding proteins are transferred into plants to enable the RNA binding proteins to be over-expressed, and the RNA binding proteins specifically recognize and bind the Y-motif element on plant growth gene mRNA to regulate and control the expression of the plant growth genes.
  7. 7. An RNA binding protein/Y-motif regulatory model, characterized in that the RNA binding protein/Y-motif regulatory model consists of an RNA binding protein and Y-motif elements, the structure of which is: ; The Y-motif element is an RNA element which is widely existing and conserved in plants and animals, the RNA binding protein is PTBP3 protein of arabidopsis thaliana, and the RNA binding protein specifically recognizes and binds to a growth gene containing the Y-motif element.
  8. 8. A nucleic acid molecule comprising a gene encoding an RNA binding protein.
  9. 9. A recombinant expression vector comprising the nucleic acid molecule of claim 8.
  10. 10. A biological material comprising the nucleic acid molecule of claim 8 or comprising the recombinant expression vector of claim 9.

Description

Application of RNA binding protein/Y-motif regulation model in plant growth-resistance tradeoff Technical Field The invention relates to the technical fields of plant molecular biology and agricultural biology, in particular to application of an RNA binding protein/Y-motif regulation model in regulating and controlling the stability of plant growth gene mRNA and balancing resistance and yield. Background In the field of crop disease-resistant genetic improvement, how to maintain growth while effectively resisting disease is a core biological problem of adapting plants to environmental stress. Activation of the plant immune system typically results in a tilting of the resource towards the defensive response, causing a significant "growth penalty", i.e. growth inhibition and yield decline. Therefore, realizing precise space-time regulation of immune output and growth related gene expression, and establishing a posttranscriptional molecular framework for regulating growth-resistance trade-off is a core problem to be solved in the field. Currently, the regulation strategies in this field are mainly focused on the transcriptional level, i.e. the transcriptional activity of genes is regulated by transcription factors. In contrast, post-transcriptional regulation, particularly RNA-binding protein (RBP) -mediated regulation of mRNA stability, translation and localization, enables precise control of protein output time and dose in a more rapid, dynamic manner, providing a more critical link to achieving such balance. The prior art has revealed the rationale for RBP to regulate expression of resistance genes by recognizing specific cis-acting elements (e.g., purine rich elements) on target mRNA. For example, in pattern-triggered immunity (PTI), a particular PAB protein may bind to AG-rich elements to facilitate selective translation of resistance genes. However, the focus of current research is generally focused on how RBP regulates expression of resistance genes, and there is no in-depth cognition and effective intervention for how the post-transcriptional fate of growth gene mRNA is regulated in the immune output context to establish a post-transcriptional molecular mechanism that fine regulates the growth-resistance tradeoff. Disclosure of Invention The invention provides an application of an RNA binding protein/Y-motif regulation model in plant growth-resistance balance. The present invention first investigated a class of specific RNA sequence elements known as Y-motif in plants. This element is widely present in mRNA of a variety of key growth genes, particularly members of the family of growth regulator genes that regulate cell proliferation and organ size. Such elements may serve as an important class of posttranscriptional regulatory nodes. Based on the above, the invention provides a brand new solution idea, namely, the RBP (for example PTBP 3) capable of identifying Y-motif can be combined with the RBP through specific intervention, so that the mRNA stability of core growth genes such as GRF can be directly regulated and controlled. When the immune is activated, the mRNA expression of the growth genes is properly regulated, so that the plant maintains effective resistance and maintains certain growth, and finally, the synergistic balance of plant disease resistance and yield potential is realized. The invention is realized by the following technology. In a first aspect of the invention, there is provided the use of an RNA binding protein/Y-motif regulatory model consisting of an RNA binding protein and Y-motif elements, the structure of which is: ; the RNA binding protein specifically recognizes and binds to the Y-motif element on plant growth gene mRNA, and regulates the expression of the plant growth gene. In a second aspect of the invention, there is provided a method of balancing plant resistance and yield, transferring a gene expressing an RNA-binding protein into a plant, over-expressing the RNA-binding protein, wherein upon immune activation, the RNA-binding protein specifically recognizes and binds to the Y-motif element on plant growth gene mRNA, and modulates expression of the plant growth gene. Further, the RNA binding protein is PTBP3 protein of arabidopsis thaliana, and the amino acid sequence of the PTBP3 protein of arabidopsis thaliana is shown as SEQ ID No. 43. Further, the nucleotide sequence of the PTBP3 protein of the arabidopsis thaliana is shown as SEQ ID No. 37. Optionally, the plant growth gene is a GRF3 gene. In a third aspect of the invention, there is provided a method of regulating expression of a plant growth gene, transferring a gene fragment expressing an RNA-binding protein into a plant to over-express the RNA-binding protein, wherein upon immune activation, the RNA-binding protein specifically recognizes and binds to the Y-motif element on plant growth gene mRNA, regulating expression of the plant growth gene. In a fourth aspect of the present invention, there is provided an