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CN-122012583-A - Application of rice gene OsUGT79 in regulation of rice seed germination under salt stress

CN122012583ACN 122012583 ACN122012583 ACN 122012583ACN-122012583-A

Abstract

The invention belongs to the technical field of genetic engineering, and discloses application of a rice gene OsUGT79 in rice seed germination under regulation and control of salt stress, wherein the nucleotide sequence of the rice gene OsUGT79 is shown as SEQ ID NO.1, and the protein sequence encoded by the rice gene OsUGT is shown as SEQ ID NO. 2. The invention has the advantages of providing new gene resources for molecular breeding and new germplasm creation related to plant salt tolerance stress germination, and having good application prospect and popularization value.

Inventors

  • CAO YUNYING
  • Ji Wanying
  • ZHANG HONGWEI
  • XU JINBIAO
  • CHEN XIAOBIN
  • WANG BAOHUA
  • ZHANG SHANPAN
  • YAO WENLI
  • XU ZHANGFEI
  • GAO JIAYUE

Assignees

  • 南通大学

Dates

Publication Date
20260512
Application Date
20260209

Claims (9)

  1. 1. Application of rice gene OsUGT to rice seed germination under regulation and control of salt stress.
  2. 2. The application of claim 1, wherein the nucleotide sequence of the rice gene OsUGT is shown as SEQ ID NO. 1.
  3. 3. The application of claim 1, wherein the protein sequence encoded by the rice gene OsUGT is shown as SEQ ID NO. 2.
  4. 4. The method according to claim 1, wherein the germination rate and the germination speed of rice seeds under salt stress are improved by over-expression of the rice gene OsUGT.
  5. 5. The method according to claim 1, wherein the plant overexpression vector of the rice gene OsUGT is introduced into rice to improve the germination rate and the germination speed of rice seeds under salt stress.
  6. 6. The method of claim 1, wherein the CRISPR/Cas9 gene editing mutant of the rice OsUGT79 gene is knocked out, so that the germination rate and the germination speed of rice seeds under salt stress are reduced.
  7. 7. The method of claim 5, wherein the plant over-expression vector of the rice gene OsUGT is introduced into rice by agrobacterium-mediated genetic transformation.
  8. 8. The method for obtaining the nucleotide sequence of the rice gene OsUGT according to claim 2, wherein the method comprises the steps of extracting total RNA from japonica rice variety Japanese sunny, performing reverse transcription to synthesize cDNA, and amplifying by using specific primers to obtain the full-length cDNA sequence of OsUGT79 gene.
  9. 9. The method according to claim 7, wherein the full-length cDNA sequence of OsUGT79 is 1401bp.

Description

Application of rice gene OsUGT79 in regulation of rice seed germination under salt stress Technical Field The invention relates to the technical field of genetic engineering, in particular to application of a rice gene OsUGT79 in rice seed germination under regulation and control of salt stress. Background Seed germination is one of the most critical stages in the life cycle of crops, and its germination speed and consistency not only affect seedling establishment and field population structure, but also determine final yield and agricultural production benefits. For important grain crops such as rice, a stable and controllable germination process is particularly critical, and particularly in a direct seeding cultivation system, a higher requirement on seed germination capacity is provided. However, under natural environment, rice seed germination is often affected by abiotic stresses such as drought and salt stress, which can interfere with the metabolic process inside the seed, delay or inhibit germination, and even lead to a decrease in seedling establishment rate. Therefore, the environmental adaptability regulation and control of rice seed germination is realized, and the method has important significance for guaranteeing the agricultural production safety. Recent studies have shown that seed germination is not only dependent on the conversion of stored nutrients to energy and structural molecules, but also is coordinated by complex metabolic networks and signal regulation pathways. Wherein, carbohydrate metabolism plays a key role in germination, not only is used as an energy source to participate in substance conversion, but also is used as a signal factor to regulate metabolic regulation, osmotic balance and stress response process. Salt stress generally disrupts osmotic pressure and metabolic balance, resulting in significant changes in metabolic pathways associated with sugar transport, signal regulation, and stored substance mobilization. Glycosylation modification is an important regulation mode of a sugar metabolism network, can influence the activity, stability and availability of a substrate, and therefore has potential important roles in germination and stress response processes. UDP-glycosyltransferases (UDP-glycosyltransferases, UGTs) are important members of plant glycosylation systems and can regulate the status and function of various metabolites, signal molecules and stress response related substances. Studies have shown that different UGT genes are involved in the tolerance mechanism of salt stress of plants, such as UDP-glycosyltransferase UGT79B2 and UGT79B3 (AtUGT B2/B3) in arabidopsis, respond to various stresses such as low temperature, salt and drought, whereas part of UGT members may be involved in seed dormancy and germination regulation, such as in arabidopsis, atUGT C5 is capable of catalyzing the conversion of abscisic acid (ABA) to its glycosylation inactivated form ABA-GE, thereby regulating endogenous ABA levels, the expression changes of which significantly affect seed dormancy depth and germination rate, atUGT E2 alters ABA and auxin signal balance by mediating the glycosylation of auxin precursor IBA, thereby promoting seed germination. However, compared with model plants, the functional research of UGT family in seed germination stage in rice is still relatively limited, and the action path and regulation mechanism of the UGT family are not systematically analyzed. Disclosure of Invention The invention aims to provide the application of the rice gene OsUGT to the germination of rice seeds under the regulation and control of salt stress, and can provide new gene resources for molecular breeding and new germplasm creation related to plant salt tolerance adversity germination, thereby having good application prospect and popularization value. The technical aim of the invention is realized by the following technical scheme: Application of rice gene OsUGT to rice seed germination under regulation and control of salt stress. Preferably, the nucleotide sequence of the rice gene OsUGT79,79 is shown as SEQ ID NO. 1. Preferably, the protein sequence coded by the rice gene OsUGT79,79 is shown as SEQ ID NO. 2. Preferably, the germination rate and the germination speed of rice seeds under salt stress are improved through the overexpression of the rice gene OsUGT79,79. Preferably, the plant over-expression vector of the rice gene OsUGT79,79 is introduced into rice, so that the germination rate and the germination speed of rice seeds under salt stress are improved. Preferably, the CRISPR/Cas9 gene editing mutant of the rice OsUGT79 gene is knocked out, so that the germination rate and the germination speed of rice seeds under salt stress are reduced. Preferably, the plant over-expression vector of the rice gene OsUGT is introduced into rice by adopting an agrobacterium-mediated genetic transformation method. Preferably, the nucleotide sequence of the rice gene OsUGT is obtained by