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CN-122012586-A - Application of gene MtPrx to regulation and control of growth and development of forage grass and stress resistance

CN122012586ACN 122012586 ACN122012586 ACN 122012586ACN-122012586-A

Abstract

The invention provides an application of a gene MtPrx in regulating and controlling forage grass growth and stress resistance, belongs to the field of genetic engineering, and relates to a gene CDS sequence shown in SEQ ID NO. 1, a coded protein amino acid sequence shown in SEQ ID NO. 2, and a novel gene which has obvious difference from a homologous gene and is not reported. Experiments prove that MtPrx negative regulation of the plant height and leaf size of forage grass, 42% increase of the plant height and 38% increase of leaf area of mutants Mtprx-1 and Mtprx-2 inhibiting the functions of the forage grass, and simultaneously the positive regulation of drought resistance of the gene, high leaf drying rate, high relative conductivity and low chlorophyll content of the functional deletion mutant under drought stress, and the invention provides key gene targets and technical supports for cultivating new varieties of high-biomass drought-resistant forage grass through genetic engineering, and has wide application prospect.

Inventors

  • SUN ZHANMIN
  • BAI LIU
  • WU YANMIN
  • WANG XUKAI
  • LIANG ZHE
  • SHI FENGLING
  • YUN LAN
  • LIN ZHICHEN
  • ZHANG LINA

Assignees

  • 中国农业科学院生物技术研究所
  • 内蒙古农业大学

Dates

Publication Date
20260512
Application Date
20260213

Claims (8)

  1. 1. The application of the gene MtPrx in regulating and controlling the growth and development of forage grass and stress resistance is characterized in that the gene MtPrx is derived from medicago tribulus, and the CDS sequence of the gene is shown as SEQ ID NO. 1.
  2. 2. The use of claim 1 wherein the gene MtPrx regulates the growth of the forage grass to negative regulation of the plant height and leaf size of the forage grass, resulting in reduced plant height and leaf area of the forage grass.
  3. 3. The use of claim 1 wherein the regulation of forage growth is effected by inhibiting the function or expression of the gene MtPrx to increase the plant height and/or leaf area of the forage.
  4. 4. The use of claim 2 wherein the stress resistance is drought resistance and the gene MtPrx positively regulates drought resistance of the forage grass to enhance tolerance of the forage grass to drought environments.
  5. 5. The use according to claim 1 wherein the forage grass is alfalfa.
  6. 6. A protein encoded by the gene MtPrx as defined in claim 1, wherein the amino acid sequence is as shown in SEQ ID NO. 2.
  7. 7. A recombinant expression vector comprising the gene MtPrx of claim 1.
  8. 8. A primer pair for detecting the gene MtPrx according to claim 1, which is characterized by comprising a forward primer MtPrx-F designed based on the sequence of SEQ ID NO. 1 and a reverse primer MtPrx-R, wherein the gene sequence of the forward primer MtPrx-F is shown as SEQ ID NO. 3 and the gene sequence of the reverse primer MtPrx-48-R is shown as SEQ ID NO. 4.

Description

Application of gene MtPrx to regulation and control of growth and development of forage grass and stress resistance Technical Field The invention belongs to the field of genetic engineering, and in particular relates to application of a gene MtPrx in regulating and controlling growth and development of forage grass and stress resistance. Background Alfalfa is used as perennial high-quality leguminous forage widely planted worldwide, is rich in high-quality protein, dietary fiber and multiple vitamins, can fix nitrogen by root nodule, and has key significance in agricultural production, ecological protection and animal husbandry development. However, drought, saline-alkali stress and the like can cause alfalfa growth to be blocked, biomass to be reduced, and even death to a large area. About 20% of cultivated lands worldwide have the salinization problem, so that the key genes for regulating and controlling biomass and stress resistance are excavated, and the method has important significance for breeding stress-resistant, high-yield and high-quality alfalfa new germplasm, new materials and new varieties. Peroxidase (Peroxidases, PRX) is a thiol-based oxidoreductase whose core function is to catalyze the decomposition and conversion of reactive molecules such as hydrogen peroxide (Hydrogen Peroxide, H 2O2), organic peroxides and peroxynitrite, playing a key role in maintaining intracellular ROS and nitric oxide signal balance, immune response and redox homeostasis. In plants, the PRX gene is involved in regulating growth and development, stress tolerance, including cell wall organization and modification (e.g., lignification and cross-linking), hormone metabolism, fruit development and maturation, seed germination, senescence regulation and other growth and development links, and plays a central role in drought, cold, disease resistance and other biotic and abiotic stress responses. Because of the huge family members and the variety of functions, 138 PRX members are contained in the rice genome, 117 members are contained in the medicago truncatula, and most of the proteins in the family have unclear functions because of the complex genes and redundant functions of the peroxidase family. Disclosure of Invention In order to solve the technical problems, the invention provides an application of a gene MtPrx in regulating and controlling the growth and the development of forage grass and stress resistance, which comprises the following specific steps: The gene MtPrx is applied to regulating and controlling the growth and development of forage grass and stress resistance, the gene MtPrx is derived from medicago truncatula, and the CDS sequence of the gene is shown as SEQ ID NO. 1. In addition, the gene MtPrx regulates and controls the growth and development of the forage grass to negatively regulate and control the plant height and leaf size of the forage grass, so that the plant height of the forage grass is reduced, and the leaf area is reduced. Moreover, the regulation of forage growth is such that by inhibiting the function or expression of the gene MtPrx, an increase in the plant height and/or leaf area of the forage is achieved. And the stress resistance is drought resistance, and the gene MtPrx is used for positively regulating and controlling the drought resistance of forage grass, so that the tolerance of the forage grass to drought environment is enhanced. Moreover, the forage grass is medicago terrestris. On the other hand, the invention provides the protein coded by the gene MtPrx, and the amino acid sequence of the protein is shown as SEQ ID NO. 2. In a third aspect, the invention provides a recombinant expression vector for said gene MtPrx. In a fourth aspect, the invention provides a primer pair of the gene MtPrx, which comprises a forward primer MtPrx-F and a reverse primer MtPrx-R designed based on the sequence of SEQ ID NO. 1, wherein the gene sequence of the forward primer MtPrx-F is shown as SEQ ID NO. 3, and the gene sequence of the reverse primer MtPrx-R is shown as SEQ ID NO. 4. Compared with the prior art, the invention has the beneficial effects that: 1. The invention provides an application of a gene MtPrx in regulating and controlling the growth and development of forage grass and stress resistance, and improves the forage grass yield and the cultivation benefit by inhibiting MtPrx from expressing and cultivating high-biomass varieties, and the industrialized application of stress-resistant high-yield forage grass breeding is promoted by enhancing the expression and cultivating drought-resistant varieties in drought, semiarid and other stress producing areas, expanding the planting range of forage grass, reducing the stress and reducing the production risk, providing an accurate solution for forage grass industries under different ecological conditions. 2. The invention identifies and discloses a gene MtPrx from medicago tribulus for the first time, the CDS and the protein sequence of t