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CN-122012587-A - Corn early-flowering and yield-keeping material creation and application based on ZmRap2.7 regulatory region editing

CN122012587ACN 122012587 ACN122012587 ACN 122012587ACN-122012587-A

Abstract

The application belongs to the technical field of genetic engineering, in particular relates to creation and application of a corn early-flowering and yield-preserving material edited based on a ZmRap2.7 regulatory region, and more particularly relates to a method for regulating and controlling corn early-flowering and yield-preserving, and a biological material and application thereof. The application aims to solve the technical problem of how to prepare the corn for obtaining early-flowering and yield-keeping corn. To solve the technical problem, the application provides a method for preparing corn with early flowering and yield maintenance, which comprises the step of carrying out gene editing on a transcriptional regulatory element of ZmRap2.7 gene in target corn to obtain corn with early flowering and yield maintenance, wherein the transcriptional regulatory element comprises a Vgt1 enhancer or/and a ZmRap2.7 promoter. The application realizes the goal of early flowering and yield maintenance in agricultural production by precisely regulating and controlling the Vgt1 enhancer or the ZmRap2.7 promoter to specifically regulate and control the expression of the ZmRap2.7 gene.

Inventors

  • TIAN FENG
  • Liang yameng
  • WANG SHUMIN
  • HE JUNJIE

Assignees

  • 中国农业大学

Dates

Publication Date
20260512
Application Date
20260214

Claims (9)

  1. 1. A method for preparing corn with early flower yield maintenance is characterized by comprising the step of carrying out gene editing on a transcriptional regulatory element of ZmRap2.7 gene in target corn to obtain corn with early flower yield maintenance, wherein the transcriptional regulatory element comprises a Vgt1 enhancer or/and a ZmRap2.7 promoter, the nucleotide sequence of the Vgt1 enhancer is shown as SEQ ID NO. 5 or has more than 70% of identity with SEQ ID NO. 5 and has the same function, and the nucleotide sequence of the ZmRap2.7 promoter is shown as SEQ ID NO. 4 or has more than 70% of identity with SEQ ID NO. 4 and has the same function.
  2. 2. A method of shortening the flowering phase of maize and maintaining yield without a decrease, the method comprising the step of gene editing a transcriptional regulatory element of the zmrapi2.7 gene in the recipient maize, said transcriptional regulatory element comprising the Vgt1 enhancer or/and the zmrapi2.7 promoter of claim 1, to shorten the flowering phase of the recipient maize and maintain yield without a decrease.
  3. 3. The method of claim 1 or 2, wherein the method comprises providing a CRISPR/Cas genome editing system comprising genes for guide RNAs targeting the Vgt1 enhancer and genes for RNA-guided nucleases, introducing the CRISPR/Cas genome editing system into the maize of interest, causing the CRISPR/Cas genome editing system to transcribe the guide RNAs in the maize of interest and express the RNA-guided nucleases to effect gene editing of the Vgt1 enhancer.
  4. 4. The method of claim 1 or 2, wherein the method comprises providing a CRISPR/Cas genome editing system comprising a gene targeting a guide RNA of the ZmRap2.7 promoter and a gene of an RNA-directed nuclease, introducing the CRISPR/Cas genome editing system into the corn of interest, causing the CRISPR/Cas genome editing system to transcribe the guide RNA in the corn of interest and express the RNA-directed nuclease to effect gene editing of the ZmRap2.7 promoter.
  5. 5. Early flowering, yield-maintaining corn obtainable by the method of any one of claims 1 to 4.
  6. 6. A biomaterial associated with Vgt1 enhancer editing as claimed in claim 1, said biomaterial being at least one of: C1 A guide RNA of a CRISPR/Cas genome editing system targeting the Vgt1 enhancer; c2 Transcribable DNA molecules that transcribe C1) the guide RNA; C3 A recombinant vector comprising the transcribable DNA molecule of C2 and an effector protein (RNA-guided nuclease) expression cassette of a CRISPR/Cas genome editing system; c3 A recombinant microorganism comprising the recombinant vector of C2; C4 A plant that transcribes the guide RNA of C1); C5 A plant containing the DNA molecule of C2); c6 Plants containing the recombinant vector of C3); c7 Plants containing the recombinant microorganism of C4).
  7. 7. The zmrap2.7 promoter gene editing-related biomaterial of claim 1, which is at least one of the following: D1 A guide RNA of a CRISPR/Cas genome editing system targeting the zmrapi2.7 promoter; d2 Transcribable DNA molecules that transcribe D1) the guide RNA; d3 A recombinant vector comprising the transcribable DNA molecule of D2 and an effector protein expression cassette of a CRISPR/Cas genome editing system; d3 A recombinant microorganism comprising the recombinant vector of D2; D4 A plant that transcribes D1) the guide RNA; d5 A plant containing D2) said DNA molecule; d6 Plants containing D3) said recombinant vector; d7 Plants containing D4) said recombinant microorganism.
  8. 8. Use of the biomaterial according to claim 6 or 7 for the preparation of plants with early flowering and yield maintenance.
  9. 9. The plant of any one of claims 6 to 8 which is a monocotyledonous plant.

Description

Corn early-flowering and yield-keeping material creation and application based on ZmRap2.7 regulatory region editing Technical Field The application belongs to the technical field of genetic engineering, in particular relates to creation and application of a corn early-flowering and yield-preserving material edited based on a ZmRap2.7 regulatory region, and more particularly relates to a method for regulating and controlling corn early-flowering and yield-preserving, and a biological material and application thereof. Background Corn (Zea mays ssp. Mays) is one of the most widely planted crops worldwide. The flowering period is a key character for determining the adaptability of the corn, and proper early flowering can help the corn adapt to a high-latitude high-altitude area with a shorter growing period and promote the corn to spread from a low-latitude low-altitude origin area to a high-latitude high-altitude area. However, changes in flowering phase have an effect on yield. Early flowering materials generally shorten the growth period of corn, reduce the dry matter accumulation time, and result in severely reduced yields. VEGETATIVE TO GENERATIVE TRANSITION 1 (Vgt 1) is the first cloned maize flowering QTL in maize, which is located within a 2kb interval of approximately 70kb upstream zmrapi2.7. The flowering period of corn refers to the number of days from sowing to tassel powder scattering, and tassel powder scattering refers to the fact that main male of corn exposes anthers to half or more of main male, and flowering in the same day can be considered. The flowering period of the corn is shortened, the reproductive growth period of the corn can be advanced, the whole growth period of the corn is shortened, the climate risks (such as high-temperature drought stress on the volt days and diseases in rainy seasons) can be avoided selectively, and the multiple cropping index and the resource efficiency can be improved. In practice, however, advancing the flowering period of corn tends to result in reduced corn yield. The preparation of early-flowering and yield-maintaining corn with early flowering and no reduction in yield has important industrial value. Disclosure of Invention The application aims to solve the technical problem of how to prepare the corn for obtaining early-flowering and yield-keeping corn. In order to solve the technical problem, the application provides the following technical scheme: The application provides a method for preparing corn with early flower yield maintenance, which comprises the step of carrying out gene editing on a transcriptional regulatory element of ZmRap2.7 gene in target corn to obtain the corn with early flower yield maintenance, wherein the transcriptional regulatory element comprises a Vgt1 enhancer or/and a ZmRap2.7 promoter, the nucleotide sequence of the Vgt1 enhancer is shown as SEQ ID NO. 5 or has more than 70% of identity with SEQ ID NO. 5 and has the same function, and the nucleotide sequence of the ZmRap2.7 promoter is shown as SEQ ID NO. 4 or has more than 70% of identity with SEQ ID NO. 4 and has the same function. In the application, the flowering period of the early-flowering and yield-maintaining corn is shorter than that of the target corn, and the yield is not lower than that of the target corn. In the application, the flowering period of corn refers to the number of days from sowing to tassel powder scattering, and tassel powder scattering refers to the fact that main male of corn is exposed and anthers reach half or more of main male, and flowering in the same day can be considered. The early flowering yield maintenance in the present application is a comparison performed under comparable conditions. "comparable conditions" refers to the same or similar environmental conditions and agronomic practices used to make meaningful comparisons between two or more maize genotypes, such that neither the environmental conditions nor agronomic practices significantly promote or account for any differences observed between the two or more maize genotypes. Environmental conditions include, for example, light, temperature, water, humidity, soil, and nutrients (e.g., nitrogen and phosphorus). The present application also provides a method for shortening the flowering phase of maize and maintaining the yield unchanged, the method comprising the step of gene editing a transcriptional regulatory element of the zmrap2.7 gene in the recipient maize, the transcriptional regulatory element comprising the Vgt1 enhancer or/and the zmrap2.7 promoter described above, to shorten the flowering phase of the recipient maize and maintain the yield unchanged. Further, the method comprises the step of performing at least one of the following gene edits on the Vgt1 enhancer to obtain the early-flowering and yield-maintaining corn: A1 Providing a CRISPR/Cas genome editing system comprising a gene for a guide RNA targeting the Vgt1 enhancer and a gene for an RNA-guided nuclease; i