CN-122012588-A - Cold-resistant gene BnaMYBL of rape BnaMYBL and application thereof

Abstract

The invention discloses a rape cold-resistant gene BnaMYBL and application thereof, belonging to the technical field of biology. The invention provides an application of knocking-out BnaMYBL gene in improving the cold resistance of rape or cultivating high-cold resistance rape. The invention clones a new gene BnaMYBL related to cold resistance from rape for the first time, after the BnaMYBL gene provided by the invention is subjected to fixed-point editing in rape, the cold resistance identification result shows that the survival rate of the knocked-out mutant rape strain of BnaMYBL gene is obviously higher than that of wild type under cold damage treatment. The Bnamybl mutation system provides a precious gene resource for cultivating cold-resistant varieties, and has important significance for analyzing the cold-resistant molecular mechanism of plants.

Inventors

• LUO DAN
• LV YAN
• CHENG YONG
• ZOU XILING
• LU GUANGYUAN

Assignees

• 中国农业科学院油料作物研究所

Dates

Publication Date

20260512

Application Date

20260224

Claims (9)

1. 1. Use of a knock-out BnaMYBL gene in any one of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape; The cDNA nucleotide sequence of BnaMYBL gene is shown in SEQ ID NO. 1.
2. 2. Use of a protein encoded by the BnaMYBL gene as defined in claim 1 in any one of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape.
3. 3. Use of a recombinant vector for knocking out the BnaMYBL gene as defined in claim 1 in any of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape.
4. 4. Use of a recombinant microorganism comprising the recombinant vector of claim 3 in any of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape.
5. 5. The use according to any one of claims 1 to 4, wherein mutation of the BnaMYBL gene in canola by gene editing techniques results in a loss of function of the BnaMYBL gene expression protein such that the cold resistance of the canola is improved.
6. 6. The use of claim 5, wherein the method of causing the functional deletion of the BnaMYBL gene-expressed protein comprises insertion of a base on the target sequence.
7. 7. The use according to any one of claims 1 to 4, wherein the canola comprises brassica napus.
8. 8. A method for improving cold resistance of rape is characterized by comprising the following steps of carrying out gene editing on BnaMYBL gene in rape to delete function of BnaMYBL gene expression protein so as to improve cold resistance of rape, wherein a cDNA nucleotide sequence of BnaMYBL gene is shown as SEQ ID NO. 1.
9. 9. A method for cultivating high-cold-resistance rape is characterized by comprising the following steps of carrying out gene editing on BnaMYBL genes in rape to delete functions of BnaMYBL gene expression proteins so as to promote the cold resistance of the rape to be improved, wherein the cDNA nucleotide sequence of BnaMYBL genes is shown as SEQ ID NO. 1.

Description

Cold-resistant gene BnaMYBL of rape BnaMYBL and application thereof Technical Field The invention relates to the technical field of biology, in particular to a rape cold-resistant gene BnaMYBL and application thereof. Background Rape is one of the important oil crops. The great development of rape production has important influence on agricultural production and national economy, and has important significance on edible oil supply safety. Because of larger climate difference between regions, different rape types have different requirements on illumination and temperature, so the rape is divided into two large producing regions of winter rape and summer rape. The Yangtze river basin is used as the main planting area of winter rape, the planting area and the yield account for 90% and 91% of the total planting area and yield, and meanwhile, the Yangtze river basin is also an important rice production area, so that a cultivation system of rice-oil rotation is realized. The suitable sowing period of the directly-sowed rape is 10 months in the middle and upper ten days, and the suitable sowing period can ensure that the rape seedlings fully utilize the light resources before winter to reach larger biomass, thereby laying a foundation for each growth period after overwintering. Therefore, the growth temperature is an important factor affecting the growth of rape in seedling stage, photosynthetic efficiency and yield. At present, in order to ensure high yield and high quality of rice, a variety with a longer growth period is often selected in production, so that the rice maturation period is continuously delayed, and the rape sowing period is correspondingly delayed. The temperature of late-sowed rape is low after sowing, so that the effective accumulated temperature of rape growth is reduced, the emergence rate is low, the emergence rate is slow, the plant growth is slow, the low-temperature injury is easy to suffer, and the effective yield is difficult to form. Therefore, in order to cope with adverse effects caused by postponing of the production period, research on cold resistance of rape is not only an important point in production practice, but also one of important targets of resistance breeding of breeders. At present, the excavation of the cold-resistant gene of the rape and the related molecular mechanism research are relatively few, and the cold-resistant improvement of the rape is urgently required to be rich in gene resources. Disclosure of Invention The invention aims to provide a rape cold-resistant gene BnaMYBL and application thereof, which are used for solving the problems in the prior art, and the survival rate of a BnaMYBL gene knockout mutant rape line is obviously higher than that of a wild type rape line under cold damage treatment. In order to achieve the above object, the present invention provides the following solutions: the invention provides the use of the knock-out BnaMYBL gene in any of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape; The cDNA nucleotide sequence of BnaMYBL gene is shown in SEQ ID NO. 1. The invention also provides application of the BnaMYBL gene-encoded protein in any one of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape. The invention also provides the application of the recombinant vector for knocking out the BnaMYBL gene in any one of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape. The invention also provides the use of a recombinant microorganism comprising said recombinant vector in any of the following: (1) Improving the cold resistance of the rape; (2) Cultivating high cold resistance rape. Alternatively, the BnaMYBL gene in canola is mutated by gene editing techniques resulting in a loss of function of the BnaMYBL gene expression protein to increase the cold resistance of the canola. Optionally, the method of causing the functional deletion of the BnaMYBL gene-expressed protein includes insertion of a base on the target sequence. Optionally, the canola comprises a brassica napus. The invention also provides a method for improving the cold resistance of the rape, which comprises the following steps of carrying out gene editing on BnaMYBL genes in the rape to ensure that the function of BnaMYBL gene expression protein is deleted so as to promote the cold resistance of the rape to be improved, wherein the cDNA nucleotide sequence of BnaMYBL genes is shown as SEQ ID NO. 1. The invention also provides a method for cultivating the high-cold-resistance rape, which comprises the following steps of carrying out gene editing on BnaMYBL genes in the rape to ensure that the function of BnaMYBL gene expression protein is deleted so as to promote the cold resistance of the rape to be improved, wherein the cDNA nucleotide sequence of BnaMYBL genes is shown as SEQ ID NO. 1. The invention discloses the follow