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CN-122012589-A - Application of ZmRBG gene in improving southern rust resistance of corn

CN122012589ACN 122012589 ACN122012589 ACN 122012589ACN-122012589-A

Abstract

The invention belongs to the technical field of agricultural biology, and particularly relates to application of ZmRBG genes in improving southern rust resistance of corn. The early sequencing result shows that the gene ZmRBG is down regulated after the rust is infected by the rust, the VIGS silencing vector pCMV201-ZmRBG is constructed for phenotypic analysis, the expression level of the ZmRBG plant with the VIGS silencing is reduced, the disease symptoms of the ZmRBG plant with the CMV are aggravated after inoculation, meanwhile, the pathogenic bacteria biomass is increased, the over-expressed gene ZmRBG shows lighter disease symptoms compared with the contrast, the pathogenic bacteria biomass is obviously reduced, the gene ZmRBG is shown to improve the resistance of corn to the rust, and a novel gene resource and a novel technical path are provided for the cultivation of a new variety of the rust-resisting southern rust of corn, so that the method has important theoretical significance and application value.

Inventors

  • CAO YANYONG
  • XIN CUIPING
  • SUN XINYAN
  • HAN SHENGBO
  • YANG WENBO
  • CHENG ZEQIANG
  • ZHU MEICHEN
  • LI HUIMIN
  • WANG DAN
  • HU WEI

Assignees

  • 河南省农业科学院

Dates

Publication Date
20260512
Application Date
20260225

Claims (10)

  1. Use of zmrbg gene to increase resistance of corn to southern rust.
  2. 2. The method of claim 1, wherein the increasing of the resistance of the corn to southern rust is achieved by reducing the incidence of southern rust and reducing biomass of southern rust pathogens in the corn plant by overexpressing ZmRBG genes.
  3. 3. The method of claim 2, wherein the pathogenic bacteria of the southern rust are Puccinia multicasts.
  4. The application of the over-expression vector of the ZmRBG gene in improving the disease resistance of corn caused by rust.
  5. 5. The method according to claim 1 to 4, wherein the ZmRBG gene has the accession number LOC100194217 in NCBI.
  6. 6. GFP is transferred into tobacco leaves by using an agrobacterium-mediated method, and the infected tobacco leaves are homogenized in phosphate buffer solution and then rubbed and inoculated onto corn leaves, so that the southern rust-resistant transgenic corn plants are obtained after cultivation.
  7. 7. The method of claim 6, wherein the ZmRBG gene has an accession number LOC100194217 in NCBI.
  8. 8. The method of claim 7, wherein the infection is to co-infect tobacco leaves with SCmDu:. GFP bacterial liquid, with the silence inhibitor P22 and the enhancement protein, SCmDu:. GFP bacterial liquid OD 600 is 1.0-1.4.
  9. 9. The method of claim 8, wherein the period of infection is 5-15 days, the ratio of homogenates is 1mL phosphate buffer per 0.5 g tobacco leaf, the phosphate buffer concentration is 0.01M, and pH=7.0.
  10. 10. The method of claim 9, wherein the maize variety is inbred line B73.

Description

Application of ZmRBG gene in improving southern rust resistance of corn Technical Field The invention belongs to the technical field of agricultural biology, and particularly relates to application of a gene to improvement of resistance of corn to southern rust. Background The corn is used as grain and cash crop with the widest planting area and the highest total yield in China, and has irreplaceable strategic positions in the fields of guaranteeing national grain safety, supporting livestock breeding, industrial processing and the like. However, the frequent occurrence of corn diseases severely restricts the stable yield and the improved quality. Along with global climate change, cultivation system adjustment and variety popularization and iteration, the traditional secondary diseases gradually rise to be a main threat, wherein the southern rust disease of corn becomes one of the disastrous diseases of corn producing areas in China. The disease-resistant variety is cultivated by utilizing the disease resistance of the corn, has the advantages of lasting control effect, environmental friendliness, low cost and the like, and is the most economical and effective fundamental way for coping with the south rust disease. At present, the breeding work of the corn for resisting southern rust in China faces the double bottleneck of source resource shortage resistance and genetic foundation stenosis. Most of the anti-source materials used in the current production are derived from tropical inbred lines, the temperate anti-source germplasm which can be directly applied is very few, compared with foreign germplasm, the high-resistance materials in the corn germplasm in China are rare, and the high-resistance materials are mainly concentrated in P group materials of farmhouse species or tropical blood-bearing edges, so that the problem of anti-source singleization is outstanding. The long-term use of a single antigen is extremely easy to cause pathogenic variation of pathogenic bacteria physiological race, resulting in the loss of resistance of disease-resistant varieties. For example, the United states successfully controls southern rust for up to 30 years using the resistance gene Rpp9, which has been essentially lost in Africa, hawaii, etc., due to physiological race variation of the pathogenic bacteria in recent years. Recent researches further reveal that the rust fungus group of the Puccinia dorsum in China has differentiated a high-toxicity pedigree, can escape the identification of the existing disease-resistant genes, and aggravates the difficulty of disease-resistant breeding. Therefore, development of new source material and resistance genes is urgently needed, the disease-resistant germplasm basis of corn is widened, and core gene resources are provided for disease-resistant breeding. With the development of molecular biology technology, the identification, cloning and functional verification of resistance genes have become key means for breaking through the bottleneck of resistance sources. The cloning and application of the plant disease-resistant gene provide a core target for molecular marker assisted breeding, transgenic breeding and other accurate breeding technologies, and remarkably accelerate the breeding process. As shown in patent 202510110770.X, zmPHYLL gene is provided, which can inhibit germination of puccinia polytricha spores, improve resistance of corn plants to southern rust of corn, and provide new gene resources for breeding material creation. Patent 202110676913.5 discloses a southern rust resistance gene RppM of corn, which performs functional annotation analysis, sequence comparison analysis and expression quantity analysis on candidate genes in a positioning interval of a gene RppM, determines that two candidate genes have the function of improving the resistance of corn to southern rust, and provides new resistance source materials and gene resources for corn resistance breeding. Although some resistance genes are identified and applied, the number of cloned resistance genes is limited, and the requirements of coping with rapid mutation of pathogenic bacteria and cultivating broad-spectrum durable disease-resistant varieties are difficult to meet. In conclusion, the development of a new southern rust resistance gene, the analysis of a molecular mechanism for regulating and controlling disease resistance, and the application of the gene to breeding of disease resistant varieties through a molecular breeding technology are urgent demands in the current corn breeding field, and have important significance in guaranteeing the safe production of corn and promoting the high-quality development of seed industry. Disclosure of Invention In order to solve the problems, the invention provides an application of ZmRBG genes in improving the resistance of corn to southern rust. The technical scheme of the invention is realized as follows: The invention provides an application of ZmRBG genes in im