CN-122012591-A - Application of plant exciton peptide MsPep1 in improving salt tolerance of alfalfa

Abstract

The invention belongs to the technical field of biology, and particularly relates to an application of a plant exciton peptide MsPep to improvement of salt tolerance of alfalfa. The invention proves that the exciton peptide MsPep can effectively improve the salt tolerance of the alfalfa and has important application value for improving the growth of the alfalfa in the saline-alkali soil.

Inventors

• SUN ZHANMIN
• WU YANMIN
• WANG XIAOMIN
• LI YANAN
• YANG GUOHONG
• Ma baishun

Assignees

• 中国农业科学院生物技术研究所
• 山东和美华农牧科技股份有限公司

Dates

Publication Date

20260512

Application Date

20260227

Claims (10)

1. 1. The application of the plant exciton peptide MsPep to the positive regulation of the salt stress capability of alfalfa is characterized in that the amino acid sequence of the plant exciton peptide MsPep1 is shown as SEQ ID NO. 1.
2. 2. The application of plant exciton peptide MsPep to improving salt tolerance of alfalfa is characterized in that the amino acid sequence of MsPep1 is shown as SEQ ID NO. 1.
3. 3. The application of the plant exciton peptide MsPep to the improvement of the seed germination rate under the stress of alfalfa salt is characterized in that the amino acid sequence of the plant exciton peptide MsPep is shown as SEQ ID NO. 1.
4. 4. The application of the plant exciton peptide MsPep to improvement of seedling growth traits under alfalfa salt stress is characterized in that the amino acid sequence of the plant exciton peptide MsPep is shown as SEQ ID NO. 1.
5. 5. The application of plant exciton peptide MsPep to relieving the inhibition of salt stress to alfalfa root length is characterized in that the amino acid sequence of MsPep is shown as SEQ ID NO. 1.
6. 6. The application of plant exciton peptide MsPep to leaf wilting of alfalfa under salt stress alleviation is characterized in that the amino acid sequence of MsPep is shown as SEQ ID NO. 1.
7. 7. The application of plant exciton peptide MsPep to the reduction of the damage degree of alfalfa leaves under salt stress is characterized in that the amino acid sequence of MsPep is shown as SEQ ID NO. 1.
8. 8. The application of plant exciton peptide MsPep in preparing a product for resisting alfalfa salt stress, positively regulating alfalfa salt stress, improving salt tolerance of alfalfa, improving seed germination rate under alfalfa salt stress, improving seedling growth characteristics under alfalfa salt stress, relieving inhibition of salt stress on alfalfa root length, relieving alfalfa leaf wilting under salt stress or reducing damage degree of alfalfa leaf under salt stress is characterized in that the amino acid sequence of MsPep1 is shown as SEQ ID NO. 1.
9. 9. The use according to claim 8, wherein the product comprises plant exciton peptide MsPep1 and adjuvants thereof, wherein the adjuvants are selected from one or more of surfactants, osmolytes, stabilizers or pH buffers.
10. 10. The use according to any one of claims 1 to 9, wherein the amino acid sequence MsPep1 is a derivative protein sequence having the same activity as the amino acid sequence shown in SEQ ID No.1 by substitution, deletion or addition of one or more amino acid residues to the amino acid sequence shown in SEQ ID No. 1.

Description

Application of plant exciton peptide MsPep1 in improving salt tolerance of alfalfa Technical Field The invention relates to the technical field of biology, in particular to a plant exciton peptide MsPep1 and application thereof in improving salt tolerance of alfalfa. Background Alfalfa (Medicago sativa) has the characteristics of rich protein content, good palatability and the like, is one of forage grass with higher nutritional value in leguminous plants, is known as 'forage grass king', and is also extremely important forage grass in animal husbandry development. The saline-alkali soil in these areas occupies a relatively large area, so that the alfalfa salt tolerance is improved, and the method has important significance for sustainable development of forage grass industry and animal husbandry in China. Plant elicitor peptides (Plant Elicitor Peptides, pep) are a class of endogenous damage-associated molecular pattern (DAMP) signaling molecules generated by cleavage of a precursor protein (PROPEPs) by proteases that activate systemic immune resistance by binding to the cell membrane PEPR receptor. At present, pep peptide is deeply researched in disease resistance, msPep1 in alfalfa is separated and identified by a preliminary research result CN202511092830.6, and MsPep1 is proved to be capable of effectively improving genetic transformation efficiency of the alfalfa. However, the action mechanism and the application value of the peptide in abiotic stress, especially salt stress are not clear, and the research on the anti-salt function of the endogenous Pep peptide of alfalfa is more blank. Based on the above, the invention aims to research the influence of alfalfa exciton peptide MsPep with different concentrations on seed germination rate, seedling growth phenotype and physiological index under salt stress, so as to develop a novel, efficient and safe small peptide for improving alfalfa salt tolerance, and has important application value for improving alfalfa growth in saline-alkali soil. Disclosure of Invention Aiming at the lack of efficient and safe alfalfa salt-resistant biological agents in the prior art, the invention provides an application of plant exciton peptide MsPep1 in improving the salt tolerance of alfalfa. The invention firstly proves that the exciton peptide MsPep can enhance seed germination, root system development and photosynthetic capacity under salt stress, provides a research foundation for growth and development of alfalfa, and has important significance for sustainable development of forage industry and animal husbandry in China. The invention provides application of plant exciton peptide MsPep in forward regulation of alfalfa salt stress capability for the first time, wherein the amino acid sequence of MsPep1 is shown as SEQ ID NO. 1. Preferably, the amino acid sequence MsPep is derived protein sequence which is obtained by substituting, deleting or adding one or more amino acid residues in the amino acid sequence shown in SEQ ID NO.1 and has the same activity as the amino acid sequence shown in SEQ ID NO. 1. Preferably, the method of synthesizing MsPep a1 comprises a microbiological or chemical method of synthesis. Preferably, the microbial synthesis comprises a prokaryotic escherichia coli or fungal yeast fermentation synthesis. Preferably, the chemical synthesis method is synthesized by Fmoc solid phase chemical synthesis technology. It is another object of the present invention to provide the use of plant elicitor peptide MsPep1 for increasing the salt tolerance of alfalfa. Another object of the present invention is to provide the use of plant elicitor peptide MsPep1 to increase seed germination under alfalfa salt stress. Another object of the present invention is to provide the use of plant elicitor peptide MsPep1 for enhancing seedling growth traits in alfalfa salt stress. Another object of the present invention is to provide the use of said plant elicitor peptide MsPep1 for alleviating the inhibition of alfalfa root length by salt stress. Another object of the invention is to provide the use of plant elicitor peptide MsPep1 for alleviating wilting of alfalfa leaves under salt stress. It is another object of the present invention to provide the use of plant elicitor peptide MsPep1 for reducing the extent of damage to alfalfa leaves under salt stress. Further, the invention also provides a product for resisting alfalfa salt stress, positively regulating alfalfa salt stress, improving salt tolerance of alfalfa, improving seed germination rate under alfalfa salt stress, improving seedling growth characteristics under alfalfa salt stress, relieving inhibition of salt stress on alfalfa root length, relieving alfalfa leaf wilting under salt stress or reducing damage degree of alfalfa leaf under salt stress, wherein the product comprises the plant elicitor peptide MsPep1 and auxiliary materials thereof. Preferably, the auxiliary material is selected from one or more of a surfac