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CN-122012605-A - Application of tea CsTPR gene in regulation and control of tea leaf tooth density

CN122012605ACN 122012605 ACN122012605 ACN 122012605ACN-122012605-A

Abstract

The invention relates to application of a tea CsTPR gene in regulating and controlling tea leaf tooth density, and belongs to the technical field of genetic engineering. The complete sequence of the tea CsTPR gene is obtained by cloning the sequence of the tea CsTPR gene. Subsequently, bioinformatics analysis is carried out on the arabidopsis thaliana, a plant expression vector is constructed, a target fragment is connected to start GUS reporter gene expression, and the arabidopsis thaliana is genetically transformed by using the recombinant plant vector, so that the function of CsTPR is verified. The characters of CsTPR38,38 transgenic lines are analyzed, the leaf tooth density is measured, and the tea tree CsTPR gene is found to be capable of regulating and controlling the leaf tooth density, so that a foundation is laid for cultivating a new variety by utilizing the CsTPR gene.

Inventors

  • Yao Xinzhuan
  • CHEN YU
  • Lv Litang
  • LI JIAYI
  • Kuang Guangqin
  • TANG HU
  • SHI GUOLI

Assignees

  • 贵州大学

Dates

Publication Date
20260512
Application Date
20260414

Claims (10)

  1. 1. The application of the tea CsTPR gene in regulating and controlling the leaf tooth density of tea leaves is characterized in that the nucleotide sequence of the tea CsTPR gene is shown as SEQ ID No. 1.
  2. 2. The use according to claim 1, wherein the CDS sequence of the tea CsTPR gene is shown in SEQ ID No. 2.
  3. 3. The use according to claim 2, wherein the amino acid sequence of the tea CsTPR gene is shown in SEQ ID No. 3.
  4. 4. The use according to claim 1, wherein CsTPR gene expression is inhibited in tea tree, and the number of tea tree leaf teeth is significantly increased.
  5. 5. The use according to claim 1, wherein CsTPR gene expression is inhibited in tea tree, and tea tree leaf tooth depth is significantly increased.
  6. 6. A recombinant plasmid for use in inhibiting the expression of CsTPR gene.
  7. 7. The recombinant plasmid according to claim 6, wherein the recombinant plasmid is pSH 737-35S-CsTPR-GUS.
  8. 8. The method for regulating and controlling the leaf tooth density of tea CsTPR genes is characterized by comprising the following steps: (1) Introducing the recombinant plasmid of claim 6 or 7 into an agrobacterium competent cell to obtain a recombinant cell; (2) And (3) carrying out vacuum dip-dyeing on tea tree branches by the recombinant cells, and then culturing.
  9. 9. Use of the recombinant plasmid according to claim 6 or 7 for increasing the number of teeth in tea leaves.
  10. 10. Use of the recombinant plasmid according to claim 6 or 7 for increasing the tooth depth of tea leaves.

Description

Application of tea CsTPR gene in regulation and control of tea leaf tooth density Technical Field The invention relates to the technical field of genetic engineering, in particular to application of a tea CsTPR gene in regulating and controlling tea leaf tooth density. Background The tea leaves, which are one of three beverages in the world, have higher economic and cultural values. The quality of the tea is not only related to economic benefit, but also related to the nutritive value of the tea. The quality is the comparison grade of the tea products. For example, among the tea leaves, there are superior black tea and inferior black tea, and there are superior green tea and inferior green tea. The quality refers to a comprehensive expression of appearance and internal quality. The quality of tea is a specific embodiment of the physical properties and main chemical components of tea. The market value of different quality grades in the tea leaves is also different, and the higher the quality grade of the tea leaves is, the larger the market value is. The national rating standard for tea is generally sensory evaluation, namely, the aspects of appearance, color, taste, purity and the like of the tea are considered, and the tea tooth density is closely related to the tea product aiming at the existing researches (shape, purity, color, aroma, taste) on the application of the tea technology. Previous studies on gene regulation of tea quality are mainly on the content of tea, and currently, studies on the appearance of tea under gene regulation are very few. Based on this, the present application has been proposed. Disclosure of Invention The invention aims to provide an application of a tea CsTPR gene in regulating and controlling leaf tooth density of tea leaves, wherein the tea CsTPR gene can regulate and control leaf tooth density, and a foundation is laid for cultivating new varieties of tea. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides application of a tea tree CsTPR gene in regulating and controlling tea leaf tooth density, and the nucleotide sequence of the tea tree CsTPR gene is shown as SEQ ID No. 1. Preferably, the CDS sequence of the tea CsTPR gene is shown in SEQ ID No. 2. Preferably, the amino acid sequence of the tea CsTPR gene is shown in SEQ ID No. 3. Preferably, csTPR gene expression is inhibited in tea tree, and the number of tea tree leaves is significantly increased. Preferably, csTPR gene expression is inhibited in tea tree, and tea tree leaf tooth depth is significantly increased. The invention provides a recombinant plasmid which is used for inhibiting CsTPR gene expression. Preferably, the recombinant plasmid is pSH 737-35S-CsTPR-GUS. The invention provides a method for regulating and controlling tea leaf tooth density by using a tea CsTPR gene, which comprises the following steps: (1) Introducing the recombinant plasmid into an agrobacterium competent cell to obtain a recombinant cell; (2) And (3) carrying out vacuum dip-dyeing on tea tree branches by the recombinant cells, and then culturing. The invention also provides application of the recombinant plasmid in improving the number of teeth of tea leaves. The invention also provides application of the recombinant plasmid in improving the tooth depth of tea leaves. The invention has the following technical effects and advantages that the complete sequence is obtained by cloning the sequence of the tea CsTPR (tetratricopeptide repeat protein-like isotom X3) gene. Subsequently, bioinformatics analysis is carried out on the arabidopsis thaliana, a plant expression vector is constructed, a target fragment is connected to start GUS reporter gene expression, and the arabidopsis thaliana is genetically transformed by using the recombinant plant vector, so that the function of CsTPR is verified. The characters of CsTPR38,38 transgenic lines are analyzed, the leaf tooth density is measured, and the tea tree CsTPR gene is found to be capable of regulating and controlling the leaf tooth density, so that a foundation is laid for cultivating a new variety by utilizing the CsTPR gene. Drawings FIG. 1 shows the first leaf of the tea tree shoot with VIGS silencing CsTPR, wherein WT, pTRV 2-CsTPR-1 are sequentially shown from left to right; FIG. 2 shows the VIGS silencing CsTPR tea tree shoot first leaf unit She Chishu; FIG. 3 shows the first leaf tooth depth of the tea tree shoot with VIGS silencing CsTPR; FIG. 4 shows the relative expression of the first leaf CsTPR gene of the tea tree branch with the VIGS silence CsTPR; FIG. 5 shows leaf teeth of a conventional tea tree variety (Yunwu Wang Chong), wherein the leaf teeth are named bird king No. 2, bird Wang Chong, bird king No. 30, bird king No. 9, bird Wang Chong and scale of 1000 μm from left to right; FIG. 6 shows the relative expression level of the second leaf unit She Chishu and CsTPR gene of the conventional tea tre