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CN-122012606-A - Application of acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding

CN122012606ACN 122012606 ACN122012606 ACN 122012606ACN-122012606-A

Abstract

The invention discloses an application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding, wherein the acid phosphatase OsPAP23 is discovered to have organophosphorus hydrolase activity for the first time and participates in rice phosphorus absorption and utilization regulation, so that the expression of the acid phosphatase OsPAP23 gene is increased in plants, the acid phosphatase activity of rice is improved, and further, the in-vivo phosphorus utilization and the utilization efficiency of organophosphorus are improved. The invention enriches the research of the phosphorus absorption and transport regulation mechanism, provides a reference mechanism for the corresponding phosphorus deficiency stress of rice, and the created PAP23 over-expression strain is expected to show growth and yield advantages in the field of applying organic fertilizer.

Inventors

  • MAO CHUANZAO
  • ZHAO KEJU
  • REN MEIYAN
  • Bu Ziqi
  • Wang Zhuyufan
  • ZHU JIANSHU

Assignees

  • 浙江大学

Dates

Publication Date
20260512
Application Date
20260414

Claims (6)

  1. 1. An application of acid phosphatase OsPAP23 gene in efficient breeding of rice phosphorus.
  2. 2. The use according to claim 1, wherein the nucleotide sequence of the acid phosphatase OsPAP23 gene is shown in SEQ ID No. 1.
  3. 3. The use according to claim 1, wherein the acid phosphatase OsPAP23 has the amino acid sequence shown in SEQ ID No. 2.
  4. 4. The use according to claim 1, wherein the use is to overexpress the acid phosphatase OsPAP23 gene in rice genome, thereby improving phosphorus uptake and utilization efficiency of rice.
  5. 5. A method for improving phosphorus uptake and utilization efficiency of rice by using the acid phosphatase OsPAP23 gene according to claim 1, wherein the method is characterized in that the acid phosphatase OsPAP23 gene is overexpressed in rice genome.
  6. 6. The method according to claim 5, wherein the method comprises the steps of connecting an acid phosphatase OsPAP23 gene to a pCAMBIA1300-35s-GFP vector, transforming escherichia coli, drawing a plasmid after correct sequencing, transferring agrobacterium EHA105, transferring the vector into a rice gene by using an agrobacterium-mediated method, and constructing a rice plant with improved phosphorus absorption and utilization efficiency.

Description

Application of acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding Technical Field The invention belongs to the field of genetic engineering, and in particular relates to application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding, wherein the gene of the rice OsPAP23 is cloned through a reverse genetics way, the function of the gene is identified through a super-expression technology and a gene editing technology, and phosphorus absorption and utilization efficiency of rice is improved by utilizing the gene. Background Phosphorus (P) is a macronutrient element necessary for plant growth and development, and plays a vital role in the formation of many biomacromolecules such as DNA, RNA, proteins, and phospholipids, but also is directly involved in regulating energy metabolism, photosynthesis, signal transduction, and other biological processes of plants. Most of phosphorus absorbed by plants is soluble inorganic phosphorus (Pi), which is an important factor for limiting plant growth and yield because it is easily sequestered by metal ions due to its extremely low diffusion coefficient, although it is abundant in soil. Besides inorganic phosphorus, the soil contains 30-65% of organic phosphorus, depending on the organic matter content of the soil. Acid phosphatase (APase) is used as a key enzyme for hydrolyzing organic phosphorus compounds, can catalyze substrates such as phosphoric monoester, phosphoric anhydride and the like to release soluble inorganic phosphorus, and plays an important role in the process of obtaining and reutilizing plant phosphorus. Among them, purple acid phosphatases (Purple acid phosphatases, PAPs) are a family of plant acid phosphatases that have the largest regulation, and are significantly induced to be expressed under low-phosphorus stress. Cloning new acid phosphatase gene, analyzing its function and creating high-efficiency phosphorus crop variety. Disclosure of Invention The invention aims to provide an application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding, and the invention screens the acid phosphatase OsPAP23 participating in rice phosphorus absorption and utilization regulation by a reverse genetics method, and the overexpression of the OsPAP23 can remarkably improve the activity of the acid phosphatase of rice, thereby improving the utilization rate of organic phosphorus and having great application potential in molecular breeding. The technical scheme adopted by the invention is as follows: The invention provides an application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding. Furthermore, the acid phosphatase OsPAP23 has organophosphorus hydrolase activity, the amino acid sequence is shown as SEQ ID NO. 2, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 1. Because of the specificity of the nucleotide sequence, any variant of the polynucleotide shown in SEQ ID NO. 1, as long as it has more than 90% homology with the polynucleotide, falls within the scope of the present invention. A variant of the polynucleotide refers to a polynucleotide sequence having one or more nucleotide changes. Variants of nucleotides include substitution variants, deletion variants and insertion variants. Variants of the polynucleotide do not substantially alter the function of the amino acid they encode. As known in the art, an allelic variant is a substitution of a polynucleotide, which may be a substitution, deletion, or insertion of one or more nucleotides, without substantially altering the function of the encoded amino acid. Furthermore, the application is that the acid phosphatase OsPAP23 gene is overexpressed in the rice genome, so that the phosphorus absorption and utilization of the rice are improved. The invention provides a method for improving phosphorus uptake of rice by using an acid phosphatase OsPAP23 gene, wherein the method is to overexpress the acid phosphatase OsPAP23 gene in a rice genome. Furthermore, the method comprises the following steps of connecting an acid phosphatase OsPAP23 gene into a pCAMBIA1300-35s-GFP vector, transforming escherichia coli, drawing a plasmid after sequencing correctly, transferring the agrobacterium EHA105, transferring the vector into a rice gene by using an agrobacterium-mediated method, and constructing a rice plant with improved phosphorus absorption and utilization efficiency. Compared with the prior art, the invention has the beneficial effects that the acid phosphatase OsPAP23 is discovered to have the activity of organophosphorus hydrolase for the first time and participates in the phosphorus absorption and utilization regulation of rice, the expression of the acid phosphatase OsPAP23 gene is increased in a plant body, the activity of the acid phosphatase of the rice is improved, the utilization efficiency of the organophosphorus is further improved, and the highest concentration of inorganic phosphorus in leaves can