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CN-122012635-A - Method for fermenting trachinotus ovatus by-product by clostridium butyricum, high-value product and application

CN122012635ACN 122012635 ACN122012635 ACN 122012635ACN-122012635-A

Abstract

The invention discloses a method for fermenting trachinotus ovatus by-products by clostridium butyricum, a high-value product and application thereof, and belongs to the technical field of microbial fermentation and resource utilization. The invention directly uses the trachinotus ovatus processing byproducts as fermentation substrates, optimizes the clostridium butyricum fermentation medium composition and fermentation conditions through a response surface method system, and establishes a high-efficiency fermentation process. The technology is adopted to convert the trachinotus ovatus by-product into a high added value fermentation product. Compared with a control group, the test result shows that the clostridium butyricum biomass in the fermentation product is improved by 13%, the butyric acid content is improved by 102.5%, the n-valeric acid, the isovaleric acid and the caproic acid content are all obviously improved in a breakthrough manner, the total antioxidant capacity of the fermentation product is improved by 67%, and the activities of superoxide dismutase and catalase are respectively improved by 105% and 83.9%. The invention realizes the high-value utilization of aquatic byproducts, and the obtained product can be used in the fields of feed, functional food and the like.

Inventors

  • DING XIAN
  • HUANG HAONAN
  • ZHANG DIANCHANG
  • GUO HUAYANG

Assignees

  • 中国水产科学研究院南海水产研究所

Dates

Publication Date
20260512
Application Date
20260212

Claims (9)

  1. 1. A method for preparing a high value added trachinotus ovatus byproduct fermentation product, which is characterized by comprising the following steps: (1) Providing oval pompano processing byproducts, grinding, sterilizing and dissolving to prepare an initial fermentation culture medium; (2) Preparing a fermentation medium based on the initial fermentation medium; (3) Taking clostridium butyricum as a fermentation strain, and optimizing the composition of a fermentation medium and/or fermentation process conditions by adopting a response surface method to obtain optimal fermentation parameters; (4) And carrying out clostridium butyricum anaerobic fermentation on the fermentation medium according to the optimal fermentation parameters to obtain the fermentation product.
  2. 2. The method of claim 1, wherein in step (3), the response values optimized by the response surface method comprise a bacterial biomass of clostridium butyricum and/or a total antioxidant capacity of the fermentation system.
  3. 3. The method of claim 1, wherein optimizing the composition of the fermentation medium in step (3) comprises supplementing the carbon source, the nitrogen source and the deoxidizer thereto, and wherein the optimal fermentation parameters comprise supplementing the carbon source with a concentration of 15-22 g/L, supplementing the nitrogen source with a concentration of 16-20 g/L and supplementing the deoxidizer with a concentration of 0.7-1.1 g/L.
  4. 4. The method of claim 3, wherein the supplemental carbon source is maltose, the supplemental nitrogen source is beef powder, and the supplemental deoxidizer is L-cysteine hydrochloride.
  5. 5. The process of claim 1, wherein in step (4), the anaerobic fermentation is carried out at a temperature of 35-38deg.C for a period of 72 h.
  6. 6. A fermentation broth obtainable by the process according to any of claims 1-5.
  7. 7. Use of the fermentation broth of claim 6 for the preparation of a functional food that contributes to antioxidant properties.
  8. 8. Use of the fermentation broth of claim 6 for the preparation of an antioxidant drug.
  9. 9. Use of the fermentation broth of claim 6 for the preparation of an animal feed.

Description

Method for fermenting trachinotus ovatus by-product by clostridium butyricum, high-value product and application Technical Field The invention relates to the technical field of microbial fermentation and resource utilization, in particular to a method for fermenting trachinotus ovatus by-products by clostridium butyricum, a high-value product and application thereof. Background The oval pompano (Trachinotus ovatus) is commonly called as golden pompano, is an important marine economic fish, and processing byproducts such as fish heads, fish bones, fish fins, fish scales, fish tails and the like can be generated in the processing production process of the oval pompano, the processing byproducts account for about 35% of the total fish weight, the oval pompano processing byproducts contain abundant protein resources, and if the byproducts are discarded at will, serious environmental pollution pressure is brought, and resource waste is also caused. At present, few researches on processing and utilizing processing byproducts of the trachinotus ovatus are carried out, namely, processing the trachinotus ovatus into fish meal or further extracting crude enzyme, grease, collagen or gelatin, however, related researches on using the processing byproducts of the trachinotus ovatus as a substrate, utilizing clostridium butyricum fermentation to realize high yield of butyric acid, cooperatively generating medium-long chain fatty acids such as valeric acid and caproic acid and simultaneously greatly improving the antioxidant activity of the product through a system optimization means such as a response surface method and the like have not been reported. Clostridium butyricum (Clostridium butyricum), an obligate anaerobic gram-positive bacillus, has remarkable probiotic properties and is widely colonized in the digestive tract of humans and various animals. In the low-oxygen microenvironment, the clostridium butyricum generates short-chain fatty acids (SCFAs) mainly comprising butyric acid through metabolic conversion, and the short-chain fatty acids can obviously reduce the pH value of intestinal tracts and form an acidic environment which is unfavorable for the colonization of conditional pathogenic bacteria. Meanwhile, clostridium butyricum has remarkable repairing effect on intestinal mucosa tissues, and the metabolic product butyric acid serving as an important energy source for regeneration of intestinal epithelial cells can accelerate the repairing process of damaged tissues by promoting protein synthesis and maintaining the structural integrity of cells, and is vital to maintaining the intestinal health of animals. However, according to the current research, the metabolites of clostridium butyricum are mainly acetic acid, propionic acid and lactic acid with shorter carbon chain length, the research on the fermentation production of pentanoic acid or hexanoic acid with longer carbon chain by clostridium butyricum has not been reported yet, and short chain fatty acids such as pentanoic acid and hexanoic acid have special regulation effects on the intestinal structure function and flora composition of animals in the breeding industry. Therefore, there is a need to provide a method for producing a high-value product by fermenting trachinotus ovatus by-products with clostridium butyricum, thereby realizing high-value utilization. Disclosure of Invention The invention aims to provide a method for fermenting trachinotus ovatus by-product by clostridium butyricum, and a high-value product and application thereof, so as to solve the problems in the prior art, the invention successfully converts the low-value trachinotus ovatus byproducts into high-value microbial fermentation products, opens up a new path for recycling the aquatic product processing byproducts, and accords with the strategy of circular economy and sustainable development. In order to achieve the above object, the present invention provides the following solutions: the invention provides a method for fermenting trachinotus ovatus by-products by clostridium butyricum, which comprises the following steps: (1) Providing oval pompano processing byproducts, grinding, sterilizing and dissolving to prepare an initial fermentation culture medium; (2) Preparing a fermentation medium based on the initial fermentation medium; (3) Taking clostridium butyricum as a fermentation strain, and optimizing the composition of a fermentation medium and/or fermentation process conditions by adopting a response surface method to obtain optimal fermentation parameters; (4) And carrying out clostridium butyricum anaerobic fermentation on the fermentation medium according to the optimal fermentation parameters to obtain the fermentation product. Further, in the step (3), the response value optimized by the response surface method comprises the biomass of the thallus of clostridium butyricum and/or the total antioxidant capacity of the fermentation system. Further, in step (3), optimiz