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CN-122012650-A - Method for preparing bacterial nanocellulose by utilizing animal slaughtering tail water

CN122012650ACN 122012650 ACN122012650 ACN 122012650ACN-122012650-A

Abstract

The invention discloses a method for preparing bacterial nanocellulose by utilizing animal slaughtering tail water, and relates to the technical field of biological manufacturing. The method comprises the steps of centrifuging animal slaughtering tail water, carrying out alkaline proteolysis, inactivating and membrane filtration pretreatment to obtain filtrate, mixing the filtrate, glucose solution and absolute ethyl alcohol under aseptic conditions, inoculating acetobacter xylinum, carrying out static culture to obtain a bacterial cellulose wet membrane, carrying out alkaline cooking purification and high-pressure microjet homogenization treatment on the wet membrane to obtain bacterial nanocellulose aqueous dispersion, carrying out surface grafting on bacterial nanocellulose and nano TiO 2 by using a silane coupling agent to introduce double bonds, carrying out ternary radical copolymerization with a dodecafluoroheptyl methacrylate monomer, displacing by tertiary butanol, and carrying out freeze drying to obtain the bacterial nanocellulose material. The invention realizes the high-value utilization of slaughter tail water, and effectively improves the hydrophobicity and swelling resistance of the product through surface chemical modification.

Inventors

  • ZHANG XIAOLONG
  • SONG XINFENG

Assignees

  • 海南拜科瑞生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260209

Claims (10)

  1. 1. A method for preparing bacterial nanocellulose by using animal slaughter tail water, which is characterized by comprising the following steps: (1) Centrifuging tail water of slaughtering animals, heating supernatant, regulating pH to be alkaline, adding alkaline protease for enzymolysis, heating to inactivate enzyme and denature impurity proteins, cooling, regulating pH to be weak acidity, standing, and performing membrane filtration to obtain refined tail water filtrate; (2) Adding disodium hydrogen phosphate and magnesium sulfate into the refined tail water filtrate, regulating pH, sterilizing to obtain solution A, sterilizing glucose solution alone to obtain solution B, selecting absolute ethyl alcohol as solution C, mixing solution A, solution B and solution C under aseptic condition, inoculating Acetobacter xylinum, and standing for culturing to obtain bacterial cellulose wet film; (3) Boiling the bacterial cellulose wet film with alkali liquor, washing to neutrality, mashing to prepare suspension, and homogenizing by high-pressure microjet to obtain bacterial nano cellulose aqueous dispersion; (4) After centrifuging the bacterial nanocellulose aqueous dispersion, re-dispersing the bacterial nanocellulose aqueous dispersion in a mixed solvent of ethanol and water, adding a silane coupling agent KH570, pre-hydrolyzing under an acidic condition, heating for reflux reaction, and after the reaction is finished, centrifugally washing to obtain bacterial nanocellulose with surface grafted double bonds; (5) Dispersing nano TiO 2 in absolute ethyl alcohol, adding a silane coupling agent KH570, carrying out heating reflux reaction under an acidic condition, and centrifugally washing after the reaction to obtain surface modified TiO 2 ; (6) Dispersing the bacterial nanocellulose with the surface modified TiO 2 and the surface grafted double bond in tetrahydrofuran, adding a dodecafluoroheptyl methacrylate monomer and an initiator azodiisobutyronitrile, heating under inert atmosphere to perform polymerization reaction, centrifuging after the reaction is finished, cleaning with tetrahydrofuran, replacing with tertiary butanol, and finally performing freeze drying to obtain the bacterial nanocellulose.
  2. 2. The method for preparing bacterial nanocellulose as claimed in claim 1 wherein in step (1), the animal slaughter tail water is livestock, poultry, seafood or river fresh slaughter tail water.
  3. 3. The method for preparing bacterial nanocellulose by utilizing animal slaughter tail water as claimed in claim 1, wherein in the step (1), the alkaline protease is added in an amount of 0.2-0.4wt% of the tail water.
  4. 4. The method for preparing bacterial nanocellulose by utilizing animal slaughter tail water as claimed in claim 1, wherein in the step (2), the adding amount of disodium hydrogen phosphate is 0.4-0.6wt% of the mass of the refined tail water filtrate; the addition amount of the magnesium sulfate is 0.2-0.4wt% of the mass of the refined tail water filtrate.
  5. 5. The method for preparing bacterial nanocellulose by utilizing animal slaughter tail water as claimed in claim 1, wherein in the step (4), the mass ratio of the bacterial nanocellulose to a silane coupling agent KH570 is 10 (1-3).
  6. 6. The method for preparing bacterial nanocellulose by utilizing animal slaughter tail water according to claim 1, wherein in the step (5), the mass ratio of nano TiO 2 to silane coupling agent KH570 is 5 (0.3-0.6).
  7. 7. The method for preparing bacterial nanocellulose by utilizing animal slaughter tail water according to claim 1, wherein in the step (6), the mass ratio of the bacterial nanocellulose with surface grafted double bonds to the surface modified TiO 2 is 2.5 (0.2-0.5).
  8. 8. The method for preparing bacterial nanocellulose by utilizing animal slaughter tail water according to claim 1, wherein in the step (6), the mass ratio of the bacterial nanocellulose with surface grafted double bonds to the dodecafluoroheptyl methacrylate is 2.5 (6-10).
  9. 9. The method for preparing bacterial nanocellulose as claimed in claim 1 wherein in step (6), styrene and divinylbenzene are added to the reaction liquid before heating to perform polymerization reaction.
  10. 10. The method for preparing bacterial nanocellulose by utilizing animal slaughtering tail water as claimed in claim 1, wherein in the step (6), the mass ratio of styrene to divinylbenzene is 10 (0.5-1.0).

Description

Method for preparing bacterial nanocellulose by utilizing animal slaughtering tail water Technical Field The invention relates to the technical field of biological manufacturing, in particular to a method for preparing bacterial nanocellulose by utilizing animal slaughtering tail water. Background Bacterial Nanocellulose (BNC) is a natural nanoscale biomass material synthesized by microbial metabolism, and the molecular chains of the bacterial nanocellulose are connected by beta-1, 4 glycosidic bonds, so that the bacterial nanocellulose has the characteristics of high crystallinity, excellent mechanical strength, good biocompatibility, degradability and the like, and has wide application prospects in the fields of medical dressings, food packaging, biosensors and the like. Compared with plant-derived cellulose, the bacterial nanocellulose has higher purity and length-diameter ratio, and has a unique three-dimensional network structure, thus being an ideal substrate of high-performance functional materials. In the process of slaughtering animals (such as pigs, cattle, sheep and the like, poultry such as chickens, ducks and the like, seafood, fresh foods such as edible fishes and the like), a large amount of blood-containing tail water is generated, and the tail water is rich in biomass nutrient components such as protein, polypeptide, nitrogen, phosphorus and the like, has the characteristics of high COD value and high grease content, and belongs to typical high-concentration organic wastewater. The traditional treatment process adopts a biochemical method or a physicochemical-biochemical combination process, so that the treatment cost is high, and the problems of nitrogen and phosphorus resource waste, easy initiation of water eutrophication and the like exist. In recent years, researchers begin to explore the preparation of bacterial cellulose by taking animal slaughtering tail water as a culture medium raw material so as to realize the resource utilization of wastes, and as the natural bacterial nanocellulose has a great number of hydrophilic hydroxyl groups on the surface of a molecular chain, the natural bacterial nanocellulose has extremely strong water adsorption and permeation capacity and has swelling resistance which is difficult to meet the practical application requirements all the time. Disclosure of Invention The invention aims to provide a method for preparing bacterial nanocellulose by utilizing animal slaughtering tail water, so as to solve the technical problems in the prior art. In order to achieve the above purpose, the present invention provides the following technical solutions: a method for preparing bacterial nanocellulose by utilizing animal slaughter tail water, comprising the following steps: (1) Centrifuging tail water of slaughtering animals, heating supernatant, regulating pH to be alkaline, adding alkaline protease for enzymolysis, heating to inactivate enzyme and denature impurity proteins, cooling, regulating pH to be weak acidity, standing, and performing membrane filtration to obtain refined tail water filtrate; (2) Adding disodium hydrogen phosphate and magnesium sulfate into the refined tail water filtrate, regulating pH, sterilizing to obtain solution A, sterilizing glucose solution alone to obtain solution B, selecting absolute ethyl alcohol as solution C, mixing solution A, solution B and solution C under aseptic condition, inoculating Acetobacter xylinum, and standing for culturing to obtain bacterial cellulose wet film; (3) Boiling the bacterial cellulose wet film with alkali liquor, washing to neutrality, mashing to prepare suspension, and homogenizing by high-pressure microjet to obtain bacterial nano cellulose aqueous dispersion; (4) After centrifuging the bacterial nanocellulose aqueous dispersion, re-dispersing the bacterial nanocellulose aqueous dispersion in a mixed solvent of ethanol and water, adding a silane coupling agent KH570, pre-hydrolyzing under an acidic condition, heating for reflux reaction, and after the reaction is finished, centrifugally washing to obtain bacterial nanocellulose with surface grafted double bonds; (5) Dispersing nano TiO 2 in absolute ethyl alcohol, adding a silane coupling agent KH570, carrying out heating reflux reaction under an acidic condition, and centrifugally washing after the reaction to obtain surface modified TiO 2; (6) Dispersing the bacterial nanocellulose with the surface modified TiO 2 and the surface grafted double bond in tetrahydrofuran, adding a dodecafluoroheptyl methacrylate monomer and an initiator azodiisobutyronitrile, heating under inert atmosphere to perform polymerization reaction, centrifuging after the reaction is finished, cleaning with tetrahydrofuran, replacing with tertiary butanol, and finally performing freeze drying to obtain the bacterial nanocellulose. In the technical scheme, the bottom sediment and insoluble blood clots are removed by centrifuging animal slaughter tail water, pH of supernat