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CN-122012659-A - Sipunculus nudus peptide and extraction method and application thereof

CN122012659ACN 122012659 ACN122012659 ACN 122012659ACN-122012659-A

Abstract

The invention relates to the technical field of peptide extraction, in particular to Sipunculus nudus peptide, an extraction method and application thereof. The preparation method comprises the specific technical scheme of 1) crushing raw materials of Sipunculus nudus, adding water, adding sodium bisulfate and ascorbic acid, stirring and soaking for 10-15min, 2) grinding the soaked raw materials, adjusting the pH value of the slurry to be alkaline, 3) adding alkaline enzyme into the slurry for microwave enzymolysis for 45-50min, adding papain B for continuous microwave enzymolysis for 45-50min, heating for inactivation, cooling, centrifugally filtering, and collecting filtrate, and 4) sequentially carrying out microfiltration, ultrafiltration and nanofiltration on the obtained filtrate. The invention adopts microwave radiation to assist enzymolysis, which not only can provide energy for an enzymolysis system and maintain the temperature required by enzymolysis, but also greatly improves the enzymolysis efficiency, and the prepared Sipunculus nudus peptide can stabilize iron ions, can develop a safe and efficient iron supplementing preparation, can comprehensively repair the reproductive function reduction caused by obesity, has multidimensional conditioning advantages, and is suitable for treating metabolic related reproductive disorders.

Inventors

  • ZHU PENG
  • LOU JUNHONG
  • Lei Xiaocan
  • LIU JINFENG
  • SHANG WEI
  • Liang Loufeng
  • LIN WENRUI
  • LIU XIN
  • XU YOUHOU
  • PAN YONGYI

Assignees

  • 北部湾大学
  • 广西赣华真美生物科技有限公司

Dates

Publication Date
20260512
Application Date
20260104

Claims (10)

  1. 1. The extraction method of the Sipunculus nudus peptide is characterized by comprising the following steps of: 1) Pulverizing Sipunculus nudus raw material, adding water, adding sodium bisulphite and ascorbic acid, stirring, and soaking for 10-15min; 2) Grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline at 10-50 ℃; 3) Adding alkaline enzyme into the slurry, stirring at power of 2-10kw, performing microwave enzymolysis for 45-50min, adding papain B, continuing stirring, performing microwave enzymolysis for 45-50min, heating, inactivating, cooling to 45-50deg.C, centrifuging, filtering, and collecting filtrate; 4) And sequentially carrying out microfiltration, twice ultrafiltration and nanofiltration on the obtained filtrate to obtain the Sipunculus nudus peptide.
  2. 2. The extraction method according to claim 1, wherein in the step 1), the Sipunculus nudus raw material is crushed to 50-100 meshes, water is added in an amount 10-40 times the weight of the Sipunculus nudus raw material, and the temperature of the water is 25-30 ℃.
  3. 3. The extraction method according to claim 1, wherein in the step 1), the addition amount of sodium bisulphite is 3-4 per mill of the mass of the raw material of Sipunculus nudus, and the addition amount of ascorbic acid is 0.5-1 per mill of the mass of the raw material of Sipunculus nudus.
  4. 4. The extraction method according to claim 1, wherein in the step 3), the temperature of the stirring microwave enzymolysis is 50-55 ℃, the temperature of the continuous stirring microwave enzymolysis is 55-60 ℃, and the temperature of the heating inactivation is 85-90 ℃.
  5. 5. The extraction method according to claim 1, wherein in the step 3), the alkaline enzyme is carsberg protease or novo protease, the addition amount is 1% -3% of the weight of the raw material of Sipunculus nudus, and the addition amount of the papain B is 2% -4% of the weight of the raw material of Sipunculus nudus.
  6. 6. The method according to claim 1, wherein in the step 4), the microfiltration is performed by using a 30000 membrane with an interceptable molecular weight of 30000Da, the two ultrafiltration operations are 10000 membranes for the first ultrafiltration and 5000 membranes for the second ultrafiltration, and 300Da membranes for the nanofiltration.
  7. 7. A Sipunculus nudus peptide extracted by the extraction method of any one of claims 1-6.
  8. 8. A Sipunculus nudus peptide powder, which is characterized in that the Sipunculus nudus peptide extracted by the extraction method of any one of claims 1-6 is obtained by freeze-drying.
  9. 9. Use of a Sipunculus nudus peptide extracted by the extraction method according to any one of claims 1-6 or a Sipunculus nudus peptide powder according to claim 8 in the preparation of an iron chelator or in the preparation of a medicament for the treatment of TM3 cell low viability and TM4 cell low viability caused by palmitic acid.
  10. 10. Use of the Sipunculus nudus peptide extracted by the extraction method according to any one of claims 1-6 or the Sipunculus nudus peptide powder according to claim 8 for preparing medicine for treating obesity induced blood sugar increase, sperm quantity decrease, sperm malformation, sperm motility low, testis index low, epididymal index low.

Description

Sipunculus nudus peptide and extraction method and application thereof Technical Field The invention relates to the technical field of peptide extraction, in particular to Sipunculus nudus peptide, an extraction method and application thereof. Background The naked Sipunculus nudus (school name: sipunculus nudus) are commonly called as sand worms, sea ginseng, sand intestines, female and male variants, are widely distributed in tropical, subtropical and temperate sea areas of the world, and are mainly distributed in Hainan, taiwan, guangdong, guangxi, fujian, shandong and other places in China, wherein the natural resources of the North Bay sea areas (Guangxi, guangdong and Hainan) are the most abundant, and are Sipunculus nudus families and Sipunculus animals. The warm water male and female species of Sipunculus nudus mainly live on coastal sandy mud bottom coasts, and feed on benthic diatoms and organic chips, and are distributed in the whole intertidal zone, so that the yield near the submarines is high. The adult Sipunculus nudus has a body length of 10-25 cm, a hole depth of 50-80 cm, a round shape, an inclined front end and an almost vertical tail end, and is called as "flower eye" by fishermen. The salinity of Sipunculus nudus is 20-35%. Sipunculus nudus is a green healthy seafood rich in various nutrients, and has crisp and tender meat and fresh and delicious taste. The history records that Sipunculus nudus have the effects of promoting lactation, nourishing yin, reducing pathogenic fire, clearing lung and resolving phlegm, and are called as "sea medicine" in ancient times in China. The coastal fishermen in southeast China is called as "ocean Cordyceps", and the southern Min people is called as "animal Ginseng". At present, the Sipunculus nudus medicinal ingredient research shows that the polysaccharide extract in the Sipunculus nudus medicinal ingredient has the effect of promoting the proliferation of mouse lymphocytes, is beneficial to monitoring the immune system and eliminating pathological cells, and plays an important role in the immune system of the organism. Liu Yongjiang et al found by analyzing the nutrient content of Sipunculus nudus that the body contains abundant amino acids and vitamin B2, which can enhance the exercise endurance and anti-fatigue ability of mice. Zheng Zhihong research on the wound healing mechanism of mice shows that Sipunculus nudus contains rich arginine and proline and has the effect of promoting wound healing. Cao Yupo by analyzing the nutrient protein of Sipunculus nudus, sipunculus nudus is found to have the function of enhancing phagocytosis and immunity of cells. In addition, sipunculus nudus has the effects of delaying aging, resisting radiation, resisting oxidation, etc. Therefore, sipunculus nudus has various organism regulating functions and has the potential of being developed into health-care food or medicine for regulating the functions of human bodies. However, the current processing and utilization research on the extraction and application of the polypeptide of the Sipunculus nudus is less, various polypeptide active substances beneficial to human bodies of the Sipunculus nudus are excavated, and the extraction and application of the Sipunculus nudus polypeptide is beneficial to human health, so that the method is one of the directions of deep processing and utilization of the Sipunculus nudus. Disclosure of Invention Aiming at the defects of the prior art, the invention provides the Sipunculus nudus peptide, the extraction method and the application thereof, and the Sipunculus nudus peptide is extracted from Sipunculus nudus dry/fresh insects by adopting a microwave enzymolysis technology, so that the Sipunculus nudus peptide can be widely used for the treatment and health care of sub-health people. In order to achieve the above purpose, the invention is realized by the following technical scheme: the invention discloses a method for extracting Sipunculus nudus peptide, which comprises the following steps: 1) Pulverizing Sipunculus nudus raw material, adding water, adding sodium bisulphite and ascorbic acid, stirring, and soaking for 10-15min; 2) Grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline at 10-50 ℃; 3) Adding alkaline enzyme into the slurry, stirring at power of 2-10kw, performing microwave enzymolysis for 45-50min, adding papain B, continuing stirring, performing microwave enzymolysis for 45-50min, heating, inactivating, cooling to 45-50deg.C, centrifuging, filtering, and collecting filtrate; 4) And sequentially carrying out microfiltration, twice ultrafiltration and nanofiltration on the obtained filtrate to obtain the Sipunculus nudus peptide. Preferably, in the step 1), the raw material of the Sipunculus nudus is crushed to 50-100 meshes, the amount of water added is 10-40 times of the weight of the raw material of the Sipunculus nudus, and the temperature of the water is 25-30 ℃. Preferably, in t