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CN-122012661-A - Method for preparing insect protein peptide by using bacillus bailii JAAS-22

CN122012661ACN 122012661 ACN122012661 ACN 122012661ACN-122012661-A

Abstract

The invention discloses a method for preparing insect protein peptide by utilizing bacillus belay JAAS-22, which comprises the following steps of (1) carrying out expansion culture on bacillus belay JAAS-22 strain to obtain bacillus belay JAAS-22 fermentation culture solution, (2) homogenizing insect larvae in a colloid mill to obtain insect pulp, (3) mixing the insect pulp with bacillus belay JAAS-22 fermentation culture solution uniformly, carrying out enzymolysis fermentation to obtain an insect pulp enzymolysis fermentation product, and (4) carrying out dialysis treatment on the insect pulp enzymolysis fermentation product to obtain the insect protein peptide with the molecular weight less than or equal to 30 kDa. The invention can efficiently prepare the bioactive peptide with stable and good antibacterial activity.

Inventors

  • LIN FENG
  • GUO TING
  • LI YUTING
  • HUANG AIXIA
  • ZHOU DONGREN
  • WANG YUCHEN
  • ZHANG HAIQI

Assignees

  • 浙江省淡水水产研究所(浙江省淡水渔业环境监测站)
  • 江苏省农业科学院

Dates

Publication Date
20260512
Application Date
20260413

Claims (8)

  1. 1. A method for preparing an insect protein peptide by using bacillus bailii JAAS-22, which is characterized by comprising the following steps: (1) Performing amplification culture on bacillus bailii JAAS-22 strains to obtain bacillus bailii JAAS-22 fermentation culture solution; (2) Homogenizing insect larvae in a colloid mill to obtain insect slurry; (3) Uniformly mixing insect pulp and bacillus bailii JAAS-22 fermentation culture solution, and performing enzymolysis fermentation to obtain an insect pulp enzymolysis fermentation product; (4) Dialyzing the insect pulp enzymolysis fermentation product to obtain insect protein peptide with molecular weight less than or equal to 30 kDa.
  2. 2. The method according to claim 1, wherein Bacillus bailii JAAS-22 is designated by its classification Bacillus velezensis and deposited with the Guangdong province microorganism strain collection on the 8 th month 10 day 2023 under the deposit number GDMCC No:63724.
  3. 3. The method according to claim 1, wherein step (1) is specifically: selecting bacillus bailii JAAS-22 single colony, inoculating the bacillus bailii JAAS-22 single colony into an LB liquid culture medium, placing the bacillus bailii JAAS-22 single colony into a constant temperature incubator for culture, wherein the culture temperature is 32-37 ℃, the stirring rotation speed is 100-200 rpm, and the culture time is 20-36 h, so as to obtain bacillus bailii seed liquid; Inoculating bacillus bailii seed liquid into a fermentation tank filled with LB liquid culture medium in an inoculum size of 1% -5% by volume, wherein the culture temperature is 32-40 ℃, the stirring speed is 100-400 rpm, the air ventilation is 0.5-2 vvm, and the culture time is 20-30 h, so as to obtain bacillus bailii JAAS-22 fermentation culture liquid.
  4. 4. The method according to claim 1, wherein 30-80mL of bacillus beijerinus fermentation broth is added per 1kg of insect pest pulp in step (3).
  5. 5. The method of claim 1, wherein in the step (3), the fermentation temperature of the enzymolysis and fermentation is 30-45 ℃ and the fermentation time is 12-36 h.
  6. 6. The method of claim 1, wherein the insect larvae are fly larvae or hermetia illucens larvae.
  7. 7. The method of claim 1, wherein the insect larvae are larvae of 4-6 days of age.
  8. 8. The method of claim 1, wherein in the step (4), the insect pulp enzymolysis and fermentation product is subjected to dialysis treatment to obtain the insect protein peptide with the molecular weight of 3-10 kDa.

Description

Method for preparing insect protein peptide by using bacillus bailii JAAS-22 Technical Field The invention relates to the technical field of fermentation engineering, in particular to a method for preparing insect protein peptide by utilizing bacillus bailii JAAS-22. Background The insect resource utilization technology is a research hotspot in the field of current organic waste treatment and sustainable protein development. Especially, larvae (fly maggots) of houseflies (Musca domastica) and larvae (Hermetia illucens) of black soldiers can efficiently utilize kitchen waste, livestock and poultry manure and agricultural byproducts for assimilation growth due to strong ecological transformation capability, and can be converted into high-added value insect proteins. Insect proteins are thus considered as strategic novel protein sources to address global protein resource starvation, replacing traditional fish meal and soybean meal. However, the current deep processing utilization of fly maggots and hermetia illucens proteins mainly has the following technical state and defects: 1. application level is elementary, and product has single function Currently, commercial applications of insect proteins have focused mainly on the primary processing level. The main technical route is that the harvested insect bodies are washed, dried (such as hot air drying and microwave drying) and mechanically crushed to prepare insect whole powder or defatted insect powder, and the insect whole powder or defatted insect powder is directly added as a feed raw material. Although research shows that the primary product can replace part of fish meal or bean pulp in daily ration to a certain extent, and improve the growth performance and intestinal health of the cultured animals, the primary product remains in the protein raw material replacement stage. Because of lack of directional intervention and separation of active ingredients in the protein, the obtained product has single function, and cannot accurately meet the differentiated requirements of high-end cultivation, pet foods and functional food additives. 2. The traditional processing technique destroys the endogenous functional activity The fly maggots and the hermetia illucens form a unique innate immune system in the long-term evolution process, so that the fly maggots and the hermetia illucens naturally enrich various functional peptide fragments in vivo, such as antibacterial peptides (AMPs), anti-inflammatory peptides, antioxidant peptides and the like. However, the existing conventional processing method has obvious disadvantages: Physical damage, namely, the traditional high-temperature drying process is extremely easy to cause the space structure of active substances such as thermosensitive antibacterial peptide and the like to be destroyed, so that the active substances lose biological activity. The extraction is low-efficiency, the conventional crushing and simple water extraction method is difficult to destroy the cell walls of the insects and active ingredients firmly combined with protein, so that a large amount of endogenous functional peptides with high added value are abandoned along with the insect residues, and cannot enter the final product, thereby causing great waste of precious bioactive resources. 3. Lack of directional enzymolysis and controllable release technology In order to achieve a conversion from "crude proteins" to "functional bioactive peptides", modern biotechnology often employs enzymatic methods. However, the existing enzymolysis technology aiming at insect proteins is still not mature, and the enzymolysis technology of plant proteins or fish proteins is mostly used, so that a specific enzyme system cannot be screened aiming at the unique amino acid composition and peptide bond structure of the fly maggots and hermetia illucens proteins. 4. Industrialized integration process deficiency When preparing functional insect bioactive peptide (fly maggot and hermetia illucens source bioactive peptide), the prior art lacks a set of efficient, controllable and industrialized preparation process, and is difficult to convert insect protein resources into protein peptide products with stable and definite functions. In view of the above, developing a process capable of efficiently preparing stable and well-defined bioactive peptides from fly maggots and hermetia illucens is a technical problem to be solved. Disclosure of Invention The invention aims to provide a method for preparing insect protein peptide by utilizing bacillus bailii JAAS-22, which can efficiently prepare bioactive peptide with stable and good antibacterial activity. The technical scheme adopted for solving the technical problems is as follows: A method for preparing an insect protein peptide by using bacillus bailii JAAS-22, comprising the following steps: (1) Performing amplification culture on bacillus bailii JAAS-22 strains to obtain bacillus bailii JAAS-22 fermentation culture soluti