CN-122012721-A - Application of SNP molecular marker related to nitrogen apparent digestibility on pig chromosome 16

Abstract

The invention discloses an application of SNP molecular markers related to nitrogen apparent digestibility on a No. 16 chromosome of a pig, wherein the locus of the SNP molecular markers is rs337476742, the SNP molecular markers correspond to T > A mutation at 7907717 bp on a No. 16 chromosome of an 11.1 version 16 of an international pig reference genome, and the genotypes of the SNP molecular markers are TT, TA or AA. The SNP molecular marker provided by the invention is obviously related to the nitrogen apparent digestibility of pigs, wherein the nitrogen apparent digestibility of pigs with the genotype of AA of the SNP molecular marker is lower than that of pigs with the genotype of AT or TT. In the breeding process, the apparent nitrogen digestibility can be improved by breeding pigs with SNP molecular marker genotype TT, the breeding process of the pigs can be accelerated, and the genetic improvement of the pigs can be realized.

Inventors

• ZUO JIANJUN
• ZHENG ENQIN
• Duan Debin
• LONG HUILING
• ZHANG ZEBIN
• Hong Genghui
• YAO ZEJIE
• LIN KAIXIA
• Liu Yidie
• WU ZHENFANG
• WU JIAJIN
• YANG JIE
• LIU SONGTAO
• LI LEKAI
• LIU LANGQING

Assignees

• 华南农业大学

Dates

Publication Date

20260512

Application Date

20251229

Claims (9)

1. 1. The application of the product for detecting the SNP molecular marker related to the apparent nitrogen digestibility on the chromosome 16 of the pig is characterized in that the locus of the SNP molecular marker is rs337476742, and the application comprises at least one of the following (1) to (8): (1) Identifying the nitrogen apparent digestibility characteristics of the pigs; (2) Preparing a product for identifying the nitrogen apparent digestibility characteristics of pigs; (3) Auxiliary evaluation of the pig feed conversion efficiency is realized based on the identification of the nitrogen apparent digestibility of the pig; (4) Preparing a product for assisting in evaluating the conversion efficiency of pigs to feed, wherein the product realizes assisting evaluation of the conversion efficiency of the feed of the pigs based on the identification of the apparent digestibility of nitrogen of the pigs; (5) Auxiliary evaluation of the pig's digestion and utilization capacity of proteins is realized based on the identification of the nitrogen apparent digestibility of the pig; (6) Preparing a product for assisting in evaluating the digestion and utilization capacity of the pig on the protein, wherein the product realizes assisting in evaluating the digestion and utilization capacity of the pig on the basis of identifying the apparent digestibility of nitrogen of the pig; (7) Pig genetic improvement, based on breeding pigs with SNP molecular marker genotype TT, to improve the nitrogen apparent digestibility of the pigs; (8) Preparing a product for assisting in genetic improvement of pigs, wherein the product is used for assisting in genetic improvement of pigs based on identification of genotypes of the SNP molecular markers.
2. 2. The use according to claim 1, wherein the product for detecting SNP molecular markers associated with nitrogen apparent digestibility on pig chromosome 16 comprises at least one of reagents, kits, chips and devices for detecting the SNP molecular markers.
3. 3. The use according to claim 2, wherein the reagent for detecting the SNP molecular marker comprises at least one of a primer and a probe for detecting the SNP molecular marker.
4. 4. The use according to claim 3, wherein the primers for detecting the SNP molecular markers comprise an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is shown as SEQ ID NO. 2, and the nucleotide sequence of the downstream primer is shown as SEQ ID NO. 3.
5. 5. The use according to any one of claims 1 to 4, wherein the pig is a doloque pig.
6. 6. The use of claim 5 wherein the pig has a nitrogen apparent digestibility trait of about 140 days old.
7. 7. A method for genetic modification of pigs comprising the steps of: (1) Determining the genotype of SNP molecular markers related to the apparent digestibility of nitrogen on chromosome 16 of pigs; (2) Selecting individuals with SNP molecular marker genotype TT; The locus of the SNP molecular marker is rs337476742.
8. 8. The method for genetic modification of swine according to claim 7, wherein in the step (1), the method for determining the genotype of the SNP molecular markers associated with apparent digestibility of nitrogen on chromosome 16 of swine comprises the steps of: Extracting pig whole genome DNA, PCR amplifying with primer with nucleotide sequence shown as SEQ ID No. 2 and SEQ ID No. 3, sequencing the amplified product, and determining SNP molecular marker genotype related to nitrogen apparent digestibility on No. 16 chromosome of pig based on the sequencing result.
9. 9. The method of genetic modification of pigs according to claim 7 or 8, wherein the pigs are doloque pigs.

Description

Application of SNP molecular marker related to nitrogen apparent digestibility on pig chromosome 16 Technical Field The invention belongs to the technical field of molecular biology, and relates to application of SNP molecular markers which are positioned on a No. 16 chromosome of a pig and are related to the apparent digestibility of pig nitrogen. Background With the continuous increase of the global meat consumption demand, the realization of sustainable development of animal husbandry has become an urgent task for industry upgrading. In the process, the control of nitrogen emission is a key link for reducing the pollution of the cultivation environment. Pig is used as main meat source, and the utilization rate of nitrogen in feed is directly related to total nitrogen excretion and environmental pollution. Therefore, the improvement of the apparent digestibility of pigs to nitrogen is an important direction for reducing nitrogen emission and promoting a green cultivation mode. However, the trait belongs to complex quantitative traits, is regulated by multiple genes, relies on tedious and high-cost phenotype measurement in the traditional breeding method, is difficult to carry out large-scale selection, and causes slow genetic improvement process and low efficiency. Disclosure of Invention The invention aims to provide an application of SNP molecular markers which are positioned on a No. 16 chromosome of a pig and are related to the nitrogen apparent digestibility of the pig. According to one aspect of the invention, the application of a product for detecting SNP molecular markers related to nitrogen apparent digestibility on a No. 16 chromosome of a pig is provided, wherein the locus of the SNP molecular markers is rs337476742, the locus corresponds to T > A mutation at 7907717 bp on a No. 16 chromosome of an international pig reference genome 11.1 version 16, and the genotype of the SNP molecular markers is TT, TA or AA. The SNP molecular marker provided by the invention is obviously related to the nitrogen apparent digestibility of pigs, wherein the nitrogen apparent digestibility of pigs with the genotype of TT of the SNP molecular marker is higher than that of pigs with the genotypes of TA and AA. The identification of the pig nitrogen apparent digestibility character can be realized by identifying the SNP molecular marker single nucleotide polymorphism and/or the genotype of the SNP molecular marker, and in the breeding process, the nitrogen apparent digestibility of offspring pigs can be improved by breeding pigs with SNP molecular marker genotype TT, the breeding process of the pigs is accelerated, and the genetic improvement of the pigs is realized. The apparent nitrogen digestibility of the pigs is directly or indirectly reflected by production characters such as the digestion and utilization capacity of the pigs to protein, the conversion efficiency of the feed and the like, and the higher the apparent nitrogen digestibility of the pigs is, the stronger the digestion and utilization capacity of the pigs to protein is, the higher the conversion efficiency of the feed is, so that the digestion and utilization capacity of the pigs to protein and the conversion efficiency of the feed can be evaluated in an auxiliary way by identifying the apparent nitrogen digestibility characters of the pigs. Therefore, the SNP molecular marker related to the apparent nitrogen digestibility provided by the invention can be also applied to auxiliary evaluation of the digestion and utilization capacity of the pig to protein and/or the conversion efficiency of the pig to feed, and can be applied to preparation of products for auxiliary evaluation of the digestion and utilization capacity of the pig to protein and/or the conversion efficiency of the pig to feed. Therefore, the product for detecting the SNP molecular marker disclosed by the invention is applied to at least one of the following (1) - (8): (1) Identifying the nitrogen apparent digestibility characteristics of the pigs; (2) Preparing a product for identifying the nitrogen apparent digestibility characteristics of pigs; (3) Auxiliary evaluation of the pig feed conversion efficiency is realized based on the identification of the nitrogen apparent digestibility of the pig; (4) Preparing a product for auxiliary evaluation of the conversion efficiency of the pig to the feed, wherein the product realizes auxiliary evaluation of the conversion efficiency of the pig to the feed based on the identification of the apparent digestibility of nitrogen of the pig; (5) Auxiliary evaluation of the digestion and utilization capacity of the pig on the protein is realized based on the identification of the nitrogen apparent digestibility of the pig; (6) Preparing a product for assisting in evaluating the digestion and utilization capacity of the pig on the basis of identifying the nitrogen apparent digestibility of the pig, wherein the product is used for assisting in evalua